RNA sequencing reveals the developmental onset of autosomal gene expression differences in male and female extravillous trophoblasts DOI Creative Commons
Matthew J. Shannon, Alexander G. Beristain

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

ABSTRACT Background The human placenta is an essential organ for fetal development and pregnancy success. Across gestation, blastocyst-derived trophoblasts facilitate the major functions of placenta. Specifically, invasive trophoblast subtypes, called extravillous (EVT), play central roles in coordinating nutrient accessibility maternal immunomodulation to semi-allogeneic placental tissues. Like fetus, these can be chromosomally male (XY) or female (XX). While are associated with distinct gene signatures, specific differences between EVT have not been defined across first trimester. Methods To understand how differ, we subjected trimester cell preparations bulk RNA sequencing. Concurrently, publicly available single-cell sequencing datasets decidual tissues were utilized resolve differentiation subtype-specific sex differences. Candidate genes then selected immuno-localized regions populations placentas. Results We found that before week 10 both lineage cells increase expression transcripts proliferation. Sex-related within this early developmental time-point restricted residing on chromosomes. Following there a broad up-regulation linked immunoregulation EVT. However, later period, autosomal appear relation biological sex. go show sex-dependent influence EVT-maternal signalling uterus whereby pregnancies exposed demonstrate more complex MIF CD99 as well angiogenesis-associated VEGF cell-cell signals immune non-immune throughout uterus. Conclusions These findings highlight timepoint critical expression. HIGHLIGHTS First compared using transcriptomics Gestational age leading drivers variation generally arise gestation uterine crosstalk driven by arising after PLAIN ENGLISH SUMMARY temporary forms during pregnancy. Importantly, acts surrogate yet functioning systems (i.e., heart, lungs, kidneys) while they mature fetus. Because develops from embryo, it biologically female. Male placentas genetically different each other, where may differentially health through unknown mechanisms. Therefore, study aimed differ at level find but before, express repertoire cells. potentially affect interact other. Taken together, results identify instruct subtle communicate compartment Summary Statement Bulk provides comprehensive comparison uncultured HLA-G-purified derived first-trimester

Language: Английский

RNA sequencing reveals the developmental onset of autosomal gene expression differences in male and female extravillous trophoblasts DOI Creative Commons
Matthew J. Shannon, Alexander G. Beristain

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

ABSTRACT Background The human placenta is an essential organ for fetal development and pregnancy success. Across gestation, blastocyst-derived trophoblasts facilitate the major functions of placenta. Specifically, invasive trophoblast subtypes, called extravillous (EVT), play central roles in coordinating nutrient accessibility maternal immunomodulation to semi-allogeneic placental tissues. Like fetus, these can be chromosomally male (XY) or female (XX). While are associated with distinct gene signatures, specific differences between EVT have not been defined across first trimester. Methods To understand how differ, we subjected trimester cell preparations bulk RNA sequencing. Concurrently, publicly available single-cell sequencing datasets decidual tissues were utilized resolve differentiation subtype-specific sex differences. Candidate genes then selected immuno-localized regions populations placentas. Results We found that before week 10 both lineage cells increase expression transcripts proliferation. Sex-related within this early developmental time-point restricted residing on chromosomes. Following there a broad up-regulation linked immunoregulation EVT. However, later period, autosomal appear relation biological sex. go show sex-dependent influence EVT-maternal signalling uterus whereby pregnancies exposed demonstrate more complex MIF CD99 as well angiogenesis-associated VEGF cell-cell signals immune non-immune throughout uterus. Conclusions These findings highlight timepoint critical expression. HIGHLIGHTS First compared using transcriptomics Gestational age leading drivers variation generally arise gestation uterine crosstalk driven by arising after PLAIN ENGLISH SUMMARY temporary forms during pregnancy. Importantly, acts surrogate yet functioning systems (i.e., heart, lungs, kidneys) while they mature fetus. Because develops from embryo, it biologically female. Male placentas genetically different each other, where may differentially health through unknown mechanisms. Therefore, study aimed differ at level find but before, express repertoire cells. potentially affect interact other. Taken together, results identify instruct subtle communicate compartment Summary Statement Bulk provides comprehensive comparison uncultured HLA-G-purified derived first-trimester

Language: Английский

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