Specificity and diversity of Klebsiella pneumoniae phage-encoded capsule depolymerases DOI Creative Commons
Michael E. Cheetham, Yunlong Huo,

Maria Stroyakovski

et al.

Essays in Biochemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 13, 2024

Abstract Klebsiella pneumoniae is an opportunistic pathogen with significant clinical relevance. K. pneumoniae-targeting bacteriophages encode specific polysaccharide depolymerases the ability to selectively degrade highly varied protective capsules, allowing for access bacterial cell wall. Bacteriophage have been proposed as novel antimicrobials combat rise of multidrug-resistant strains. These enzymes display extraordinary diversity, and are key determinants phage host range, however limited data available our current knowledge their mechanisms predict efficacy limited. Insight into resolved structures Klebsiella-specific capsule reveals catalytic mechanisms, intra-chain cleavage mechanism providing opportunities recombinant protein engineering. A detailed comparison 58 characterised hints at structural mechanistic patterns, such conservation domains substrate recognition tethering, well diversity within groups that target same substrate. Another way understand depolymerase specificity by analyzing targeted structures, these may share similarities recognizable bacteriophage depolymerases, leading broader specificities. Although we only begun explore complexity further research essential thoroughly characterise enzymes. This will be crucial understanding predicting efficacy, engineering optimized therapeutic applications.

Language: Английский

Bacteriophage-derived depolymerase: a review on prospective antibacterial agents to combat Klebsiella pneumoniae DOI

Xin Jiao,

Menglu Wang, Yan‐Xia Liu

et al.

Archives of Virology, Journal Year: 2025, Volume and Issue: 170(4)

Published: March 8, 2025

Language: Английский

Citations

0
Phage-antibiotic combinations against Klebsiella pneumoniae: impact of methodological approaches on effect evaluation DOI Creative Commons
RB Gorodnichev, Anastasiia O. Krivulia, Maria Kornienko

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 12, 2025

Background The combined use of bacteriophages and antibiotics represents a promising strategy for combating multidrug-resistant bacterial pathogens. However, the lack uniformity in methods assessing combination effects experimental protocols has resulted inconsistent findings across studies. This study aimed to evaluate interactions between phages on Klebsiella pneumoniae strains using various statistical approaches formalize effects. Methods Effects were assessed four from distinct classes (gentamicin, levofloxacin, meropenem, chloramphenicol), three different genera (Dlv622, Seu621, FRZ284), depolymerase (Dep622) K. KL23 capsule type. Antibiotics used at C max concentrations, sublethal levels. A modified t -test, Bliss independence model, two-way ANOVA, checkerboard assay employed results. Results Among 48 combinations, 33 statistically significant, including 26 cases synergy 7 antagonism. All showed consistency identifying effects; however, -test method detected greater number strongest was observed with levofloxacin Seu621 or Dep622 all strains. Checkerboard assays confirmed selected but indicated that could vary antimicrobial concentrations. Conclusion choice analytical substantially impacts detection phage-antibiotic method, due their simplicity sensitivity, may be optimal clinical application, while ANOVA confirming strong interactions. These results emphasize need consider interaction characteristics when designing therapeutic strategies.

Language: Английский

Citations

0
Phage therapy for Klebsiella pneumoniae: Understanding bacteria–phage interactions for therapeutic innovations DOI Creative Commons
Julie Le Bris, Nathalie Chen,

Adeline Supandy

et al.

PLoS Pathogens, Journal Year: 2025, Volume and Issue: 21(4), P. e1012971 - e1012971

Published: April 8, 2025

Klebsiella pneumoniae (KP) is a Gram-negative bacterium that commonly resides in the human gastrointestinal tract and can also act as an opportunistic pathogen cause extra-intestinal infections. KP poses global health threat because it causes both hospital- community-acquired infections immune-competent immunocompromised hosts. These be multidrug-resistant and/or hypervirulent, making difficult to treat deadly. In absence of effective treatments for recalcitrant infections, bacteriophage (phage) therapy gaining attention promising alternative. this review, we evaluate epidemiology epitope diversity, discuss interactions between KP-targeting phages their bacterial hosts from eco-evolutionary perspective, summarize recent efforts phage treating We novel approaches, including genetic engineering machine learning, initial steps toward developing precision medicine approach emerging dangerous pathogen.

Language: Английский

Citations

0
A novel depolymerase encoded by phage P5054 specifically degrades the K57-type capsular polysaccharide of Klebsiella pneumoniae DOI Creative Commons

Heyuan Lun,

Juanjuan Wang,

Huagen Chen

et al.

One Health Advances, Journal Year: 2025, Volume and Issue: 3(1)

Published: April 16, 2025

Language: Английский

Citations

0
Antimicrobial potential of a novel K5-specific phage and its recombinant strains against Klebsiella pneumoniae in milk DOI Creative Commons
Pei Li,

Z. Li,

Peng Wan

et al.

Journal of Dairy Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Challenges and opportunities of phage therapy for Klebsiella pneumoniae infections DOI

Xin Kou,

Xiaoyu Yang, Rui Zheng

et al.

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

ABSTRACT Traditional antibiotics have been effective in many cases. However, the rise multidrug-resistant bacteria has diminished their therapeutic efficacy, signaling dawn of an era beyond antibiotics. The challenge multidrug resistance Klebsiella pneumoniae is particularly critical, with increasing global mortality and rates. Therefore, development alternative therapies to urgently needed. Phages, which are natural predators bacteria, inherent advantages. comprehensive information on K. phages lacking current literature. This review aims analyze summarize relevant studies, focusing present state phage therapy for infections. includes examination treatment methodologies, associated challenges, strategies, new technologies, clinical trial safety regulatory issues, future directions development. Enhancing technology crucial addressing evolving threat .

Language: Английский

Citations

1
A review of the fighting Acinetobacter baumannii on three fronts: antibiotics, phages, and nanoparticles DOI
Samane Teymouri, Maryam Pourhajibagher, Abbas Bahador

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: Oct. 8, 2024

Language: Английский

Citations

1
Phage-Based Therapy in Combination with Antibiotics: A Promising Alternative against Multidrug-Resistant Gram-Negative Pathogens DOI Creative Commons
Cleo Anastassopoulou, Stefanos Ferous,

Aikaterini Petsimeri

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(10), P. 896 - 896

Published: Oct. 14, 2024

The continued rise in antimicrobial resistance poses a serious threat to public health worldwide. use of phages that can have bactericidal activity without disrupting the normal flora represents promising alternative treatment method. This practice has been successfully applied for decades, mainly Eastern Europe, and recently used as an emergency therapy compassionate care United States. Here, we provide comprehensive review pre-clinical clinical applications phage concerning three major Gram-negative pathogens:

Language: Английский

Citations

1
Specificity and diversity of Klebsiella pneumoniae phage-encoded capsule depolymerases DOI Creative Commons
Michael E. Cheetham, Yunlong Huo,

Maria Stroyakovski

et al.

Essays in Biochemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 13, 2024

Abstract Klebsiella pneumoniae is an opportunistic pathogen with significant clinical relevance. K. pneumoniae-targeting bacteriophages encode specific polysaccharide depolymerases the ability to selectively degrade highly varied protective capsules, allowing for access bacterial cell wall. Bacteriophage have been proposed as novel antimicrobials combat rise of multidrug-resistant strains. These enzymes display extraordinary diversity, and are key determinants phage host range, however limited data available our current knowledge their mechanisms predict efficacy limited. Insight into resolved structures Klebsiella-specific capsule reveals catalytic mechanisms, intra-chain cleavage mechanism providing opportunities recombinant protein engineering. A detailed comparison 58 characterised hints at structural mechanistic patterns, such conservation domains substrate recognition tethering, well diversity within groups that target same substrate. Another way understand depolymerase specificity by analyzing targeted structures, these may share similarities recognizable bacteriophage depolymerases, leading broader specificities. Although we only begun explore complexity further research essential thoroughly characterise enzymes. This will be crucial understanding predicting efficacy, engineering optimized therapeutic applications.

Language: Английский

Citations

1