The commercial PRRSV attenuated vaccine can be a potentially effective live trivalent vaccine vector DOI Creative Commons
Yang Li, Yumiao Wang,

Xiuxiu Pei

et al.

Applied Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 109(1)

Published: May 2, 2025

PRRSV is an immunosuppressive virus that prone to secondary infection by other viral and microbial pathogens, exacerbating its harm pigs. The development of multivalent vaccines can prevent control multiple pathogens great significance for the pig industry. In order evaluate potential commercial as vectors trivalent vaccine, this study first embedded mCherry or EGFP genes fluorescent proteins into infectious clone HP-PRRSV MLV vaccine strain GD construct a recombinant strain. Furthermore, we constructed strain, rPRRSV-mCherry-EGFP, expresses both simultaneously evaluated stability immunogenicity exogenous proteins. results showed MARC-145 cells infected with rPRRSV-mCherry-EGFP could express proteins, number positive increased infection, providing more tool options visualization high-throughput screening anti-PRRSV drugs. More importantly, be passaged in at least 10 generations. It induce piglets produce antibodies against PRRSV, EGFP, mCherry, indicating two also good immune responses. Our research suggests using HP-PRRSV-attenuated vector embedding may effective attempt achieve cross-protection three important basis design vaccines. KEY POINTS: • A expressed foreign was constructed. mCherry. had used triple

Language: Английский

The commercial PRRSV attenuated vaccine can be a potentially effective live trivalent vaccine vector DOI Creative Commons
Yang Li, Yumiao Wang,

Xiuxiu Pei

et al.

Applied Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 109(1)

Published: May 2, 2025

PRRSV is an immunosuppressive virus that prone to secondary infection by other viral and microbial pathogens, exacerbating its harm pigs. The development of multivalent vaccines can prevent control multiple pathogens great significance for the pig industry. In order evaluate potential commercial as vectors trivalent vaccine, this study first embedded mCherry or EGFP genes fluorescent proteins into infectious clone HP-PRRSV MLV vaccine strain GD construct a recombinant strain. Furthermore, we constructed strain, rPRRSV-mCherry-EGFP, expresses both simultaneously evaluated stability immunogenicity exogenous proteins. results showed MARC-145 cells infected with rPRRSV-mCherry-EGFP could express proteins, number positive increased infection, providing more tool options visualization high-throughput screening anti-PRRSV drugs. More importantly, be passaged in at least 10 generations. It induce piglets produce antibodies against PRRSV, EGFP, mCherry, indicating two also good immune responses. Our research suggests using HP-PRRSV-attenuated vector embedding may effective attempt achieve cross-protection three important basis design vaccines. KEY POINTS: • A expressed foreign was constructed. mCherry. had used triple

Language: Английский

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