Sulforaphane Is Not Only a Food Supplement: It Diminishes the Intracellular Survival and Colonization of Salmonella enterica

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Sulforaphane is a main bioactive component in several edible cruciferous vegetables. It acquires benefits to health addition its considered antibacterial and antivirulence activities. Herein, we aimed at evaluating the activity of sulforaphane against worldwide clinically important enteric pathogen Salmonella enterica serovar Typhimurium. The influence on bacterial adhesion, invasion, biofilm formation, intracellular replication was assayed. Additionally, effect type III secretion system (TTSS) S. quantified. outcome combination with different antibiotics assessed, an vivo protection assay conducted assess pathogenesis. results showed significant antibiofilm subinhibitory reduction invasion inside host cells. findings emphasized decreased capacity induce pathogenesis presence sulforaphane. Our finding attributed these activities interference TTSS-type II downregulation encoding genes. In nutshell, vegetable safe adjunct therapy that can be administrated alongside traditional for treating pathogens as enterica.

Language: Английский

---

Bacterial Metabolites in Attack

Published: Jan. 1, 2025

Language: Английский

---

Reprofiling lamivudine as an antibiofilm and anti-pathogenic agent against Pseudomonas aeruginosa

AMB Express, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 22, 2025

Resistance to antibiotics is a critical growing public health problem that needs urgent action combat. To avoid the stress on bacterial growth evokes development of resistance, anti-virulence agents can be an attractive strategy as they do not target growth. There are FDA approved drugs have been screened for their activities. Lamivudine (LAM) synthetic nucleoside analogue used antiretroviral in treatment HIV and HBV. The present study aimed assess activities LAM against clinically important pathogen Pseudomonas aeruginosa. LAM's antibiofilm were evaluated. impact quorum sensing (QS) systems which control production these virulence factors was assessed virtually by quantification expression QS-encoding genes. Furthermore, vivo mice protection assay conducted attest anti-pathogenic activity. current findings elaborated promising anti-QS LAM. interfered with biofilm formation P. aeruginosa PAO1 strain. Moreover, swarming motility pyocyanin protease significantly diminished. At molecular level, downregulated genes LasI, LasR, RhlR, PqsA PqsR. Additionally, detailed silico docking simulation studies showed considered high ability bind hinder QS receptors In agreement vitro silico, results full conclusion, could repurposed employed adjunct therapy traditional treating serious pseudomonal infections.

Language: Английский

---

Dual Inhibition of Phosphodiesterase 3 and 4 Enzymes by Ensifentrine Protects against MRSA-Induced Lung Endothelial and Epithelial Dysfunction

Cells, Journal Year: 2024, Volume and Issue: 13(21), P. 1750 - 1750

Published: Oct. 23, 2024

Acute Respiratory Distress Syndrome (ARDS) is a severe lung condition with high mortality rate for which there are no effective therapeutics. The failure of the alveolar-capillary barrier, composed endothelial (EC) and alveolar epithelial (AEC) cells, critical factor leading to excessive inflammation edema characteristic acute injury (ALI) pathophysiology. Phosphodiesterases (PDE) enzymes well-recognized their roles in regulating permeability inflammation. Although PDE inhibitors used as therapeutics inflammatory diseases like COPD (chronic obstructive pulmonary disease), efficacy treating ARDS has not yet been established. In this study, we investigated effects ensifentrine, an FDA-approved novel dual 3/4 inhibitor, on dysfunction caused by methicillin-resistant

Language: Английский

---