Evaluating the Inhibitory Effects of Probiotic Bacteria and Propolis Extracts on the Growth and Histopathological Changes in Gastric Tissues of <i>Helicobacter pylori</i> Challenged Wistar Rats DOI Creative Commons

Roghayeh Kiani,

Naheed Mojgani, Farzad Kobarfard

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 23(1)

Published: Dec. 25, 2024

Helicobacter pylori is a significant contributor to range of gastrointestinal conditions, with conventional treatment methods primarily relying on antibiotics. However, the rise antibiotic-resistant strains has necessitated exploration alternative therapeutic approaches. To determine in vitro antibacterial potential probiotic bacteria (Lacticaseibacillus rhamnosus BLRH 260 and Limosilactobacillus reuteri) four propolis extracts against H. analyze their impacts body weight index histopathological changes pylori-challenged Wistar rats. The inhibitory effects (L. L. growth were evaluated using an agar well diffusion assay. In vivo analysis involved fifty-four male rats (200 - 250 g) infected suspension (108 CFU/mL) orally administered or probiotics via gavage for 21 days. different treatments gastric tissue samples assessed, results statistically analyzed. tested supernatant fluids from mentioned showed activity assay, notable variations. vivo, findings demonstrated that oral administrations probiotics, either separately combination, led increases amelioration samples, particularly terms erosion depth, hemorrhagic inflammation, apoptosis animals. Histopathological differences between antibiotic-treated animals those receiving other observed, differences. this study underscore benefits addressing pylori-induced gastropathy. Additional research necessary clarify mechanisms refine dosage protocols optimal effectiveness.

Language: Английский

Antimicrobial activity and mechanism of novel postbiotics against foodborne pathogens DOI Creative Commons

Yucui Tong,

Zaheer Abbas, Jing Zhang

et al.

LWT, Journal Year: 2025, Volume and Issue: 217, P. 117464 - 117464

Published: Jan. 31, 2025

Language: Английский

Citations

3
Integrative Roles of Functional Foods, Microbiotics, Nutrigenetics, and Nutrigenomics in Managing Type 2 Diabetes and Obesity DOI Open Access

H.‐C. Lam,

Shih‐Ping Lin, Duc-Huy T. Nguyen

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(4), P. 608 - 608

Published: Feb. 7, 2025

Diabetes and obesity are globally prevalent metabolic disorders posing significant public health challenges. The effective management of these conditions requires integrated personalized strategies. This study conducted a systematic literature review, identifying 335 relevant papers, with 129 core articles selected after screening for duplicates irrelevant studies. focus the is on synergistic roles functional foods, microbiotics, nutrigenomics. Functional including phytochemicals (e.g., polyphenols dietary fibers), zoochemicals essential fatty acids), bioactive compounds from macrofungi, exhibit potential in enhancing insulin sensitivity, regulating lipid metabolism, reducing inflammatory responses, improving antioxidant capacity. Additionally, critical role gut microbiota highlighted, as its interaction foods facilitates modulation pathways. Nutrigenomics, encompassing nutrigenetics genomics, reveals how genetic variations single-nucleotide polymorphisms (SNPs)) influence responses gene expression, forming feedback loop between habits, variations, microbiota, health. review integrates insights to propose comprehensive sustainable nutrition interventions, offering novel perspectives preventing managing type 2 diabetes obesity. Future clinical studies warranted validate long-term efficacy safety

Language: Английский

Citations

1
Probiotic-infused active packaging: preparation, characterization, application and modeling the preservation of fresh strawberries fruit DOI Creative Commons
Mostafa Rahmati-Joneidabad,

Mohammad Reza Zare Bavani,

Behrooz Alizadeh Behbahani

et al.

LWT, Journal Year: 2025, Volume and Issue: unknown, P. 117586 - 117586

Published: Feb. 1, 2025

Language: Английский

Citations

1
The Protective Effects of Lactobacillus reuteri Combined With Clostridium butyricum Miyairi 588 on Intestinal Barrier Function, Water Transport, and Oxidative Stress in a Rat Model of 5FU‐Induced Diarrhea DOI Creative Commons
Sheau‐Chung Tang,

Wen‐Chen Chuang,

Jiuan‐Miaw Liao

et al.

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(6)

Published: May 23, 2025

ABSTRACT 5‐Fluorouracil (5FU) is a commonly employed and highly effective chemotherapeutic agent in clinical oncology. Nevertheless, one of the most frequent debilitating adverse effects associated with 5FU treatment diarrhea. These gastrointestinal complications can affect patients' quality life adherence to regimens. Consequently, addressing mitigating diarrhea during therapy presents critical urgent challenge oncological care. This study investigated whether probiotic Lactobacillus reuteri combined Clostridium butyricum Miyairi 588 (LCs) alleviate 5FU‐induced potential mechanism. Wistar rats received (50 mg/kg, intraperitoneal injection) for 5 consecutive days establish colitis model. LCs were administered 15 before injection continued until day sacrifice. Tissue morphology, inflammatory oxidative stress markers, as well expression mRNA related intestinal barrier integrity, apoptosis, aquaporins (AQPs) evaluated colon tissue samples. analyses used hematoxylin eosin staining, enzyme‐linked immunosorbent assay (ELISA), quantitative real‐time reverse transcription‐polymerase chain reaction (qRT‐PCR) techniques. Additionally, concentrations short‐chain fatty acids (SCFAs) measured using gas chromatography‐flame ionization detection (GC‐FID) analysis. In this model, mitigated weight loss, diarrhea, bloody stool, shortened length, colonic histopathology. Treatment resulted reduced levels MDA, TNF‐α, IL‐1β, MPO activity, decreased IFN‐γ, AKT, NF‐κB, iNOS. significantly downregulated VCAM‐1, CXCL4, MAPK, caspase‐3, while upregulating tight junction protein occludin expression. also notably diminished AQP7, VIP, PKA. demonstrates that have therapeutic on colitis, primarily through their antioxidant properties, anti‐apoptotic effects, mucosal integrity maintenance, neutrophil infiltration reduction, cytokines aquaporin modulation.

Language: Английский

Citations

0
Postbiotic Wash Treatments: A Novel Post-Harvest Approach to Reduce Salmonella and Enhance Egg Safety DOI
Ragini Reddyvari, Mary Anne Roshni Amalaradjou

Food Control, Journal Year: 2025, Volume and Issue: unknown, P. 111398 - 111398

Published: May 1, 2025

Language: Английский

Citations

0
Evaluating the Inhibitory Effects of Probiotic Bacteria and Propolis Extracts on the Growth and Histopathological Changes in Gastric Tissues of <i>Helicobacter pylori</i> Challenged Wistar Rats DOI Creative Commons

Roghayeh Kiani,

Naheed Mojgani, Farzad Kobarfard

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 23(1)

Published: Dec. 25, 2024

Helicobacter pylori is a significant contributor to range of gastrointestinal conditions, with conventional treatment methods primarily relying on antibiotics. However, the rise antibiotic-resistant strains has necessitated exploration alternative therapeutic approaches. To determine in vitro antibacterial potential probiotic bacteria (Lacticaseibacillus rhamnosus BLRH 260 and Limosilactobacillus reuteri) four propolis extracts against H. analyze their impacts body weight index histopathological changes pylori-challenged Wistar rats. The inhibitory effects (L. L. growth were evaluated using an agar well diffusion assay. In vivo analysis involved fifty-four male rats (200 - 250 g) infected suspension (108 CFU/mL) orally administered or probiotics via gavage for 21 days. different treatments gastric tissue samples assessed, results statistically analyzed. tested supernatant fluids from mentioned showed activity assay, notable variations. vivo, findings demonstrated that oral administrations probiotics, either separately combination, led increases amelioration samples, particularly terms erosion depth, hemorrhagic inflammation, apoptosis animals. Histopathological differences between antibiotic-treated animals those receiving other observed, differences. this study underscore benefits addressing pylori-induced gastropathy. Additional research necessary clarify mechanisms refine dosage protocols optimal effectiveness.

Language: Английский

Citations

0