IL-4-primed human umbilical cord mesenchymal stem cells-derived extracellular vesicles facilitate recovery in spinal cord injury via the miR-21-5p/PDCD4-mediated shifting of macrophage M1/M2 polarization

Life Sciences, Journal Year: 2025, Volume and Issue: 364, P. 123441 - 123441

Published: Feb. 3, 2025

Language: Английский

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The role of cytokine licensing in shaping the therapeutic potential of wharton’s jelly MSCs: metabolic shift towards immunomodulation at the expense of differentiation

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 20, 2025

Cytokine licensing with pro-inflammatory molecules, such as tumour necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ), has emerged a promising strategy to enhance the therapeutic potential of multipotent mesenchymal stromal cells (MSCs). While demonstrated benefits for immunomodulation, its effects on other key MSC functions, including differentiation paracrine activity, remain incompletely explored. In this study, we evaluated transcriptomic, metabolomic, functional changes induced by short-term TNF-α/IFN-γ priming Wharton's jelly-derived MSCs (WJ-MSCs). WJ-MSCs were expanded exposed TNF-α IFN-γ (10 ng/ml each) 24 h. Transcriptomic analysis was performed using RNA sequencing identify differentially expressed genes related immune modulation lineage commitment. Metabolomic profiling conducted high-resolution mass spectrometry assess in metabolic pathways. Functional assays cytokine growth factor secretion. notable alterations gene expression, upregulating pathways linked response, inflammation, signalling. However, treatment significantly attenuated osteogenic adipogenic MSCs, evidenced reduced expression RUNX2, ALP, CEBPA, PPARG. The had negligible effect EGF, FGF-2, HGF, LIF, SCF production VEGF-A VEGF-C elevated, although levels remained low. revealed enhanced kynurenine pathway indicative increased tryptophan catabolism, accompanied elevated fatty acids polyamines. Our findings demonstrate that reprograms enhancing their immunomodulatory capacity at expense potential. These results highlight need tailored strategies optimize functionality specific clinical applications.

Language: Английский

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Mesenchymal stem cell therapy for liver transplantation: clinical progress and immunomodulatory properties

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 27, 2024

Language: Английский

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Single-cell multiomics to advance cell therapy

Cytotherapy, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

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