The lncRNA HMMR-AS1 promotes the malignant progression of ovarian cancer cells by regulating the miR-627-3p/PTN axis DOI
Jinhua He, Fei Tian, Jie Li

et al.

Published: April 21, 2025

Abstract Background Long non-coding RNAs (lncRNAs) are crucial regulators of ovarian cancer(OC), playing a significant role in malignant transformation and closely linked to poor prognosis. Therefore, it is investigate the impact lncRNAs on biological behavior cancer understand their underlying molecular mechanisms. Methods The binding sites target genes were predicted through bioinformatics analysis, gene expression levels measured using qRT-PCR. cells was assessed cell function assays. Gene targeting relationships verified dual-luciferase reporter gene(DLRG) assay. Protein analyzed Western blotting. Results Hyaluronan-mediated motility receptor antisense RNA1(HMMR-AS1) upregulated cells. Cell experiments demonstrated that HMMR-AS1 promotes behaviors DLRG targets miR-627-3p, which turn pleiotrophin(PTN). Furthermore, we discovered functions as competing endogenous RNA (ceRNA) for regulating PTN thereby promoting phenotypes Conclusions In summary, our study indicates lncRNA highly expressed plays carcinogenic role. Targeting may offer novel therapeutic strategy treating cancer.

Language: Английский

The lncRNA HMMR-AS1 promotes the malignant progression of ovarian cancer cells by regulating the miR-627-3p/PTN axis DOI
Jinhua He, Fei Tian, Jie Li

et al.

Published: April 21, 2025

Abstract Background Long non-coding RNAs (lncRNAs) are crucial regulators of ovarian cancer(OC), playing a significant role in malignant transformation and closely linked to poor prognosis. Therefore, it is investigate the impact lncRNAs on biological behavior cancer understand their underlying molecular mechanisms. Methods The binding sites target genes were predicted through bioinformatics analysis, gene expression levels measured using qRT-PCR. cells was assessed cell function assays. Gene targeting relationships verified dual-luciferase reporter gene(DLRG) assay. Protein analyzed Western blotting. Results Hyaluronan-mediated motility receptor antisense RNA1(HMMR-AS1) upregulated cells. Cell experiments demonstrated that HMMR-AS1 promotes behaviors DLRG targets miR-627-3p, which turn pleiotrophin(PTN). Furthermore, we discovered functions as competing endogenous RNA (ceRNA) for regulating PTN thereby promoting phenotypes Conclusions In summary, our study indicates lncRNA highly expressed plays carcinogenic role. Targeting may offer novel therapeutic strategy treating cancer.

Language: Английский

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