Lanreotide protects against LPS-induced inflammation in endothelial cells and mouse lungs DOI
Md Matiur Rahman Sarker,

Saikat Fakir,

Khadeja-Tul Kubra

et al.

Tissue Barriers, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Somatostatin is expressed in various tissues - including the hypothalamus and strongly suppresses Growth Hormone levels to maintain homeostasis. Synthetic somatostatin analogs are currently used clinics treat neuroendocrine tumors acromegaly. An emerging body of evidence suggests that those synthetic peptides exert anti-inflammatory activities. The present study examines effect Lanreotide (LAN) on Lipopolysaccharide (LPS)-triggered injury endothelial cells mice. Our findings indicate LAN effectively mitigates LPS-induced hyperpermeability, inflammation, reactive oxygen species (ROS) generation bovine pulmonary artery (BPAEC) human lung microvascular (HULEC-5a). A murine model acute was also utilized, examine effects edema inflammation. observations suggest myosin light chain 2 (MLC2), Cofilin, extracellular signal-regulated kinase 1/2 (ERK1/2), STAT1, STAT3, P38 activation; edema. In conclusion, based aforementioned observations, it suggested counteracts experimental

Language: Английский

Exploring the Role of the Gut Microbiota in Modulating Colorectal Cancer Immunity DOI Creative Commons
Nikolay K. Shakhpazyan, Л.М. Михалева, A.L. Bedzhanyan

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(17), P. 1437 - 1437

Published: Aug. 27, 2024

The gut microbiota plays an essential role in maintaining immune homeostasis and influencing the landscape within tumor microenvironment. This review aims to elucidate interactions between dynamics, with a focus on colorectal cancer (CRC). spans foundational concepts of immuno-microbial interplay, factors microbiome composition, evidence linking immunotherapy outcomes. Gut modulates anti-cancer immunity through several mechanisms, including enhancement surveillance modulation inflammatory responses. Specific microbial species their metabolic byproducts can significantly influence efficacy immunotherapies. Furthermore, diversity correlates clinical outcomes CRC, suggesting potential as valuable biomarker for predicting response immunotherapy. Conclusions: Understanding relationship responses offers novel therapeutic strategies development. not only influences natural history treatment CRC but also serves critical modulator activity. Further exploration into microbiome's could enhance effectiveness existing treatments guide development new modalities.

Language: Английский

Citations

10
Inhaled polystyrene microplastics impaired lung function through pulmonary flora/TLR4-mediated iron homeostasis imbalance DOI

Huiwen Kang,

Danyang Huang, Wei Zhang

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 946, P. 174300 - 174300

Published: June 25, 2024

Language: Английский

Citations

9
Integrating Microfluidics, Hydrogels, and 3D Bioprinting for Personalized Vessel-on-a-Chip Platforms DOI
San Seint Seint Aye, Zhi Fang, Mike C. L. Wu

et al.

Biomaterials Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Advancement of vascular models from simple 2D culture to complex vessel-on-a-chip platforms through integration microfluidics, biomimetic hydrogels, and 3D bioprinting, enabling controlled investigation thrombosis mechanisms.

Language: Английский

Citations

1
Protective effects of growth hormone – releasing hormone antagonists in the lungs of septic mice DOI

Saikat Fakir,

Khadeja‐Tul Kubra,

Mohammad S. Akhter

et al.

Cellular Signalling, Journal Year: 2024, Volume and Issue: 121, P. 111260 - 111260

Published: June 12, 2024

Language: Английский

Citations

8
Human Umbilical Vein Endothelial Cells as a Versatile Cellular Model System in Diverse Experimental Paradigms: An Ultrastructural Perspective DOI
Hana Ďúranová,

Lenka Kuželová,

Petra Borotová

et al.

Microscopy and Microanalysis, Journal Year: 2024, Volume and Issue: 30(3), P. 419 - 439

Published: May 31, 2024

Abstract Human umbilical vein endothelial cells (HUVECs) are primary isolated from the of an cord, extensively used in cardiovascular studies and medical research. These cells, retaining characteristics vivo, serve as a valuable cellular model system for understanding vascular biology, dysfunction, pathophysiology diseases such atherosclerosis, responses to different drugs or treatments. Transmission electron microscopy (TEM) has been cornerstone revealing detailed architecture multiple systems including HUVECs, allowing researchers visualize subcellular organelles, membrane structures, cytoskeletal elements. Among them, endoplasmic reticulum, Golgi apparatus, mitochondria, nucleus can be meticulously examined recognize alterations indicative various stimuli. Importantly, Weibel–Palade bodies characteristic secretory organelles found which easily distinguished TEM. distinctive structures also dynamically react factors through regulated exocytosis, resulting complete selective release their contents. This review summarizes ultrastructural features HUVECs highlights utility TEM pivotal tool analyzing diverse research frameworks, contributing insights into comprehension HUVEC behavior enriching our knowledge complexity biology.

Language: Английский

Citations

7
Sevoflurane alleviates inflammation, apoptosis and permeability damage of human umbilical vein endothelial cells induced by lipopolysaccharide by inhibiting endoplasmic reticulum stress via upregulating RORα DOI

Wei‐Wei Ni,

Zhiwei Zou,

Ping Jiang

et al.

Prostaglandins & Other Lipid Mediators, Journal Year: 2024, Volume and Issue: 172, P. 106821 - 106821

Published: Feb. 17, 2024

Language: Английский

Citations

5
Bacteroidetes promotes esophageal squamous carcinoma invasion and metastasis through LPS-mediated TLR4/Myd88/NF-κB pathway and inflammatory changes DOI Creative Commons
Zhongbing Wu, Jianxin Guo, Zhenhuan Zhang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: June 4, 2024

Abstract Gut microbiota plays a crucial role in gastrointestinal tumors. Additionally, gut microbes influence the progression of esophageal cancer. However, major bacterial genera that affect invasion and metastasis cancer remain unknown, underlying mechanisms unclear. Here, we investigated flora metabolites patients with squamous cell carcinoma found abundant Bacteroides increased secretion entry surface antigen lipopolysaccharide (LPS) into blood, causing inflammatory changes body. We confirmed these results mouse model 4NQO-induced situ further identified epithelial–mesenchymal transition (EMT) occurrence TLR4/Myd88/NF-κB pathway activation vitro experiments revealed LPS from fragile promoted proliferation, migration, invasion, induced EMT by activating pathway. These reveal are closely associated through higher response level signaling both common to inflammation tumors LPS, providing new biological target for prevention or treatment.

Language: Английский

Citations

5
Controlled production of lipopolysaccharides increases immune activation in Salmonella treatments of cancer DOI Creative Commons
Lars M. Howell, Simin Manole, Alec R. Reitter

et al.

Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(5)

Published: May 1, 2024

Abstract Immunotherapies have revolutionized cancer treatment. These treatments rely on immune cell activation in tumours, which limits the number of patients that respond. Inflammatory molecules, like lipopolysaccharides (LPS), can activate innate cells, convert tumour microenvironments from cold to hot, and increase therapeutic efficacy. However, systemic delivery (LPS) induce cytokine storm. In this work, we developed immune‐controlling Salmonella (ICS) only produce LPS tumours after colonization clearance. We tuned expression msbB , controls production immunogenic LPS, by optimizing its ribosomal binding sites protein degradation tags. This genetic system induced a controllable inflammatory response increased dendritic cross‐presentation vitro. The strong off state did not TNFα prevented adverse events when injected into mice. accumulation ICS intravenous injection focused responses specifically tumours. Tumour‐specific infiltration activated monocytes neutrophils, levels IL‐6, CD8 T cells draining lymph nodes. reduced growth mouse survival. By increasing efficacy bacterial anti‐cancer therapy, localized could provide options with immune‐resistant cancers.

Language: Английский

Citations

4
Recent Advances in Pathogenesis and Anticoagulation Treatment of Sepsis-Induced Coagulopathy DOI Creative Commons

Cheng Man,

Yuan An,

Guoxin Wang

et al.

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 737 - 750

Published: Jan. 1, 2025

Coagulopathy in sepsis is common and associated with high mortality. Although immunothrombosis necessary for infection control, excessive thrombus formation can trigger a systemic thrombo-inflammatory response. Immunothrombosis plays core role sepsis-induced coagulopathy, research has revealed complex interplay between inflammation coagulation. Different mechanisms underlying sepsis-related coagulopathy are discussed, including factors contributing to the imbalance of pro- anticoagulation relevant endothelial cells. The potential therapeutic implications anticoagulants on these discussed. This review contributes our understanding pathogenesis patients sepsis. Recent studies suggest that cells play an important immunoregulation hemostasis. Meanwhile, non-anticoagulation effects anticoagulants, especially heparin, which act septic patients, have been partially revealed. We believe further insights into will help physicians evaluate patient conditions effectively, leading advanced early recognition better decision-making treatment

Language: Английский

Citations

0
Suspected sepsis: patient assessment and management in the emergency department DOI

Clare Hird,

Mike Parker

Emergency Nurse, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

Sepsis is a potentially life-threatening condition triggered by infection that responsible for an estimated 48,000 deaths in the UK each year. Its pathophysiology complex, its symptomology non-specific and clinical presentations extremely varied. Despite numerous campaigns to raise awareness of sepsis, it still goes undetected. In 2024, National Institute Health Clinical Excellence revised guideline on sepsis Trust published seventh edition Manual. This article discusses how emergency nurses should assess manage patients with suspected sepsis. It describes tools available them, including Early Warning Score 2 6, emphasises importance early antibiotic administration, serial lactate measurements, source control antimicrobial stewardship.

Language: Английский

Citations

0