Conditions Necessary for the Transfer of Antimicrobial Resistance in Poultry Litter DOI Creative Commons

Aaron Oxendine,

Allison A. Walsh,

T. R. Young

et al.

Antibiotics, Journal Year: 2023, Volume and Issue: 12(6), P. 1006 - 1006

Published: June 3, 2023

Animal manures contain a large and diverse reservoir of antimicrobial resistance (AMR) genes that could potentially spillover into the general population through transfer AMR to antibiotic-susceptible pathogens. The ability poultry litter microbiota transmit was examined in this study. Abundance phenotypic assessed for antibiotics: ampicillin (Ap; 25 μg/mL), chloramphenicol (Cm; streptomycin (Sm; 100 tetracycline (Tc; μg/mL). qPCR used estimate gene load streptomycin-resistance sulfonamide-resistance aadA1 sul1, respectively, community. determined relative total bacterial abundance using 16S rRNA qPCR. Poultry contained 108 CFU/g, with Gram-negative enterics representing minor (<104 CFU/g). There high Sm (106 107 CFU/g) Tc sizeable antimicrobial-resistance regards copies per genome (aadA1: 0.0001-0.0060 sul1: 0.0355-0.2455). While plasmid observed from Escherichia coli R100, as an F-plasmid donor control, Salmonella recipient vitro, no were detected microcosm inclusion E. R100. Confirmatory experiments showed isolated bacteria not interfering filter matings. As R100 at °C, conjugative pRSA chosen its frequency (10-4 10-5) °C. strain background influenced persistence litter, ever observed. Although contains significant load, potential is low under conditions commonly assess conjugation.

Language: Английский

Prevalence and antibiotic resistance of Salmonella in organic and non-organic chickens on the Eastern Shore of Maryland, USA DOI Creative Commons
Anuradha J. Punchihewage-Don,

Jürgen Schwarz,

Abdirahman Diria

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 4, 2024

infections have been intensely increasing and becoming a universal public health crisis. This study investigated the prevalence of

Language: Английский

Citations

12
Effect of Basil, Thyme and Sage Essential Oils as Phytogenic Feed Additives on Production Performances, Meat Quality and Intestinal Microbiota in Broiler Chickens DOI Creative Commons

Petru Al. Vlaicu,

Arabela Elena Untea, Tatiana Dumitra Panaite

et al.

Agriculture, Journal Year: 2023, Volume and Issue: 13(4), P. 874 - 874

Published: April 15, 2023

As the use of antibiotics has been banned or reduced in certain countries animal industries, search for new alternatives to and will continue be a research subject poultry several years. This study aimed evaluate effect basil, thyme sage essential oils (EO) broiler chickens’ diets. A total 120 Cobb 500 chickens aged 12 days were distributed into four homogeneous groups thirty with six replicates five broilers each, raised until 42 age. For diets, corn, soybean meal, corn gluten wheat used as main ingredients control diet (CON), three experimental diets formulated follows: containing 0.05% oil from basil (EOB), (EOT), (EOS). The results showed that production performances, European Production Efficiency Factor (EPEF), Broiler Index (EBI) carcass weight improved (p < 0.05) compared control; however, no significant anatomical parts development was observed. Lightness (L *) hue angle (H colorimetric meat parameters altered but without an on texture. antioxidant capacity polyphenols content thigh n-3 n-6 polyunsaturated fatty acids higher samples control. intestinal microbiota also significantly lowering Escherichia coli, Coliforms staphylococci small intestine caecum increased lactobacilli count Overall, all EO-supplemented potential improve quality; EOS more effective altering chicken caecum.

Language: Английский

Citations

16
Growth performance, organs weight, intestinal histomorphology, and oocyst shedding in broiler chickens offered novel single strain Bacillus subtilis isolated from camel dung and challenged with Eimeria DOI Creative Commons

Alison Gelinas,

Sudhanshu Sudan, Robert Patterson

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 103(4), P. 103519 - 103519

Published: Feb. 7, 2024

We evaluated single strain Bacillus subtilis BS-9 direct-fed microbial (BSDFM) isolated from camel dung in Eimeria challenged broiler chickens. Seven-hundred d-old Ross 708 male chicks were placed pens (25 birds/pen) and allocated to two treatments (n=14). From d 0 13, control received untreated water (-BSDFM), (2) treated 2 mL x 108 colony forming unit/bird/day (+BSDFM); daily intake (WI) was recorded. On 9, birds half (+Eimeria) of per treatment 1 E. maxima acervulina oocysts orally, the other (-Eimeria) sterile saline solution. Birds had ad libitum access feed a line 14. Feed (FI), body weight (BW) mortality recorded for calculating BW gain (BWG) conversion ratio (FCR). 14 35, sample necropsied organ intestinal measurements. Excreta samples collected 19 oocyst count. There no effect (P>0.05) on growth performance or WI 0-9. interactions between BSDFM (P=0.014) 29 (P=0.036) with unchallenged +BSDFM being heavier than treatments. The main effects (P<0.05) 10-35 FI, BWG such that increased decreased (P<0.01) these parameters. interaction (P=0.022) FCR -BSDFM poor counterparts, but none differed birds. an (P=0.039) bursa exhibiting reduced (P=0.01) (P=0.002) villi height crypt depth ratio. Results showed supplementation via can support chickens may be strategy reduce adverse coccidiosis.

Language: Английский

Citations

5
Yeast mannan rich fraction positively influences microbiome uniformity, productivity associated taxa, and lay performance DOI Creative Commons
Robert J. Leigh, A. Corrigan, Richard Murphy

et al.

Animal Microbiome, Journal Year: 2024, Volume and Issue: 6(1)

Published: March 4, 2024

Abstract Background Alternatives to antibiotic as growth promoters in agriculture, such supplemental prebiotics, are required maintain healthy and high performing animals without directly contributing antimicrobial resistance bioburden. While the gut microbiota of broiler hens has been well established successfully correlated performance, our knowledge, a study yet be completed on effect prebiotic supplementation correlating mature laying hen productivity microbiota. This focused establishing impact yeast derived prebiotic, mannan rich fraction (MRF), cecal late hens. benefitted from large sample sizes so intra- intergroup variation effects could statistically accounted for. Results Taxonomic richness was significantly greater at all taxonomic ranks evenness lower for MRF-supplemented birds ( P < 0.005). Use principal coordinate analyses component found significant between treatment groups. When assessed compositional uniformity (an indicator flock health), more uniform than control species level. From food safety animal welfare perspective, Campylobacter jejuni abundance birds. In this study, associated with weight gain anticorrelator performance hens) were while health-correlated butyrate propionate producing Conclusions The use prebiotics may key factor controlling balance limiting agri-food chain pathogen persistence promoting uniformity. previous studies, increased α- β-diversity indices determinants mitigation performance. post-peak hens, across samples, pathogenic bioburden correlators

Language: Английский

Citations

5
Clostridium perfringens in the Intestine: Innocent Bystander or Serious Threat? DOI Creative Commons

Xuli Ba,

Youshun Jin,

Xuan Ning

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(8), P. 1610 - 1610

Published: Aug. 7, 2024

The Clostridium perfringens epidemic threatens biosecurity and causes significant economic losses. C. infections are linked to more than one hundred million cases of food poisoning annually, 8–60% susceptible animals vulnerable infection, resulting in an loss 6 USD. enzymes toxins (>20 species) produced by play a role intestinal colonization, immunological evasion, micro-ecosystem imbalance, mucosal disruption, all influencing host health. In recent decades, there has been increase drug resistance due antibiotic misuse bacterial evolution. At the same time, traditional control interventions have proven ineffective, highlighting urgent need develop implement new strategies approaches improve intervention targeting. Therefore, in-depth understanding spatial temporal evolutionary characteristics, transmission routes, colonization dynamics, pathogenic mechanisms will aid development optimal therapeutic vaccines for management. Here, we review global epidemiology perfringens, as well molecular features roles various virulence factors pathogenicity. addition, emphasize measures prevent this zoonotic disease reduce infection perfringens.

Language: Английский

Citations

5
Effect of oral administration of microcin Y on growth performance, intestinal barrier function and gut microbiota of chicks challenged with Salmonella Pullorum DOI Creative Commons
Wenjing Li, Zhiwei Zeng,

Di Zhou

et al.

Veterinary Research, Journal Year: 2024, Volume and Issue: 55(1)

Published: May 22, 2024

Abstract The lasso peptide microcin Y (MccY) effectively inhibits various serotypes of Salmonella in vitro, but the antibacterial effect against S . Pullorum poultry is still unclear. This study was first to evaluate safety and anti- infection MccY specific pathogen-free (SPF) chicks. test showed that body weight, IgA IgM levels serum, cecal microbiota structure 3 groups chicks orally administrated with different doses (5 mg/kg, 10 20 mg/kg) for 14 days were not significantly from those control group. Then, randomized into experiment infection: (I) negative group (NC), (II) Pullorum-challenged (SP, 5 × 8 CFU/bird), (III) MccY-treated (MccY, mg/kg). results indicated compared SP group, treatment increased weight average daily gain ( P < 0.05), reduced burden feces, liver, cecum enhanced thymus, decreased spleen liver index 0.05). Additionally, jejunal villus height, lowered ileal crypt depth upregulated expression IL-4 , IL-10 ZO-1 jejunum ileum, as well CLDN-1 0.05) Furthermore, probiotic flora Barnesiella etc.), while decreasing relative abundance pathogenic Escherichia etc.)

Language: Английский

Citations

4
Sustainable processing technologies (pulsed light, electrolysed water and ozonation) for microbial decontamination of muscle foods DOI
Nikheel Bhojraj Rathod, Slim Smaoui,

Rinku Agrawal

et al.

Innovative Food Science & Emerging Technologies, Journal Year: 2024, Volume and Issue: 96, P. 103778 - 103778

Published: Aug. 1, 2024

Language: Английский

Citations

4
A systematic literature review of forecasting and predictive models for enterococci intrusion in aquatic ecosystems DOI Creative Commons

Pharow Peter,

Edoardo Bertone, Rodney A. Stewart

et al.

Cleaner Water, Journal Year: 2025, Volume and Issue: unknown, P. 100063 - 100063

Published: Jan. 1, 2025

Language: Английский

Citations

0
Degradation of plasmid-mediated resistance genes in poultry slaughterhouse wastewater employing a UV/H2O2 process: A metagenomic approach DOI
Beatriz Oliveira de Farias, Enrico Mendes Saggioro,

Kaylanne S. Montenegro

et al.

Chemosphere, Journal Year: 2025, Volume and Issue: 372, P. 144109 - 144109

Published: Jan. 17, 2025

Language: Английский

Citations

0
Molecular Epidemiology of Type F <i>Clostridium perfringens</i> Among Diarrheal Patients and Virulence-Resistance Dynamics — 11 Provinces, China, 2024 DOI
Zelin Yan, Hanyu Wang,

Yanyan Zhu

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: 7(3), P. 69 - 76

Published: Jan. 1, 2025

Type F Clostridium perfringens (C. perfringens) represents a significant pathogen in human gastrointestinal diseases, primarily through its cpe gene encoding C. enterotoxin (CPE). This investigation examined the prevalence, antimicrobial resistance patterns, and genetic characteristics of within Chinese population. The study analyzed 2,068 stool samples collected from 11 provincial hospitals 2024. Antimicrobial susceptibility testing was conducted following Clinical & Laboratory Standards Institute (CLSI) guidelines, while whole-genome sequencing provided detailed profiles. Evolutionary relationships clonal transmission patterns were investigated phylogenetic environment analyses. prevalence 2.38%, with isolates predominantly identified clinical higher detection rates gastroenterology departments. Notably, 47.1% demonstrated high to metronidazole, all exhibited intermediate erythromycin. Phylogenetic analysis revealed similarity among patients same province (single-nucleotide polymorphism (SNPs)<100), indicated potential horizontal transfer between animal strains. cases, sporadic pets food products. These findings highlight emergence outbreaks diarrheal patients, emphasizing necessity for targeted interventions as virulence factors increase.

Language: Английский

Citations

0