Integrative genetic and epigenetic control of skeletal muscle fiber traits in agricultural animals DOI Creative Commons
Xiaolong Chang, Junwu Ma

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: May 2, 2025

Skeletal muscle fiber traits are fundamental to meat production and the quality of agricultural animals. The rich genetic resources diverse phenotypic expression in animal species provide invaluable materials for investigating molecular regulatory mechanisms underlying myofiber development characteristics, optimizing breeding strategies, developing models human muscle-related diseases. This review presents an integrative perspective on epigenetic regulation skeletal development, incorporating evolutionary, genomic, epigenomic, multi-omics insights. We focus architecture causative or candidate genes traits, as revealed by genome-wide association studies (GWAS) selective sweep signatures, underscoring their adaptive significance potential breeding. role mechanisms, such DNA methylation, histone modifications, non-coding RNAs, linking variation is also discussed. By synthesizing multi-omic data, we a comprehensive understanding networks driving growth differentiation. aims consolidate current knowledge offer actionable insights advance research, applications biomedical fields.

Language: Английский

MUSTN1 Interaction With SMPX Regulates Muscle Development and Regeneration DOI Creative Commons

Yu Fu,

Xin Hao, Peng Shang

et al.

Cell Proliferation, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 19, 2025

ABSTRACT Pigs are important agricultural animals whose growth rate and meat production performance related to muscle development. Musculoskeletal embryonic nuclear protein 1 (MUSTN1) participates in various biological processes, including myogenesis animals, but the physiological functions mechanisms of porcine MUSTN1 on development unclear; thus, we aimed elucidate them. We found that was highly expressed muscles fast‐growing pigs. Functionally, promoted myoblast proliferation differentiation. knockout mice exhibited reduced mass fibre cross‐sectional area, decreased exercise endurance, delayed regeneration. Small X‐linked (SMPX) identified as an interacting MUSTN1, its promotion myogenic differentiation depended MUSTN1. Furthermore, stabilised SMPX maintained myofiber morphology. This study suggests is a critical regulator control regeneration potential target for animal genetic improvement treatment human disease.

Language: Английский

Citations

1
Transcriptome and chromatin accessibility landscape of ovarian development at different egg-laying stages in taihe black-bone silky fowls DOI Creative Commons
Jingyi Zhang,

Yong Cui,

Jiming Ruan

et al.

Poultry Science, Journal Year: 2025, Volume and Issue: 104(3), P. 104864 - 104864

Published: Feb. 7, 2025

Language: Английский

Citations

1
Integrative Analysis of ATAC-Seq and RNA-Seq Identifies Key Genes Affecting Muscle Development in Ningxiang Pigs DOI Open Access
Wenhua Tan, Chenxi Liu,

Juan Liu

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2634 - 2634

Published: March 14, 2025

Meat production traits in pigs are critical economic characteristics, primarily influenced by the formation and development of skeletal muscle. Skeletal muscle is regulated a complex transcriptional network, which partly relies on chromatin accessibility for initiation. Ningxiang pigs, renowned Chinese indigenous breed, highly valued their tender meat. However, studies focusing particularly from perspective accessibility, have not yet been reported. Based this, present study selected several key time points to perform Transposase-Accessible Chromatin Sequencing (ATAC-seq) RNA sequencing (RNA-seq). This was carried out identify open regions genes during different growth stages, could influence pigs. We collected longissimus dorsi samples at postnatal days 14 (D14), 28 (D28), 85 (D85), 165 (D165), 250 (D250). For each age, three individuals were ATAC-seq RNA-seq. After initial differential analysis among ages, we identified 6412 differentially accessible peaks 1464 expressed genes. To clarify candidate transcription factors affecting motif revealed potential cis-regulatory elements with binding sites factors, including Fosl2 JunB. Correlation 56 overlapping significant positive correlation (r = 0.73, p 1 × 10−14) between gene expression accessibility. Key such as HOXA10, closely related development, specifically examined. These results enhance our understanding genetic epigenetic regulatory mechanisms porcine providing robust foundation future molecular studies.

Language: Английский

Citations

0
MUSTN1 and FABP3 interact to regulate adipogenesis and lipid deposition DOI Creative Commons

Yu Fu,

Xin Hao,

J. Y. Nie

et al.

Journal of Lipid Research, Journal Year: 2025, Volume and Issue: unknown, P. 100804 - 100804

Published: April 1, 2025

Lipid deposition is related to agricultural animal production and human health, elucidating its molecular regulatory mechanisms a topic of interest challenge in current scientific research. Musculoskeletal embryonic nuclear protein 1 (MUSTN1) regulates growth development, including muscle tissue; however, role fat remains unknown. Thus, our objective was determine this role. Our new findings were as follows: MUSTN1 highly expressed the tissue pigs with strong adipose capacity; functionally, promoted proliferation adipogenic differentiation porcine mouse preadipocytes. knockout mice protected against HFD-induced obesity, hepatic steatosis, insulin resistance; fatty acid binding 3 identified an interacting MUSTN1, which facilitated preadipocyte by activating phosphatidylinositol kinase/AKT signaling pathways. This study reveals positive regulator for suggesting novel approach studying obesity genetic improvement through modulation expression.

Language: Английский

Citations

0
Integrative genetic and epigenetic control of skeletal muscle fiber traits in agricultural animals DOI Creative Commons
Xiaolong Chang, Junwu Ma

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: May 2, 2025

Skeletal muscle fiber traits are fundamental to meat production and the quality of agricultural animals. The rich genetic resources diverse phenotypic expression in animal species provide invaluable materials for investigating molecular regulatory mechanisms underlying myofiber development characteristics, optimizing breeding strategies, developing models human muscle-related diseases. This review presents an integrative perspective on epigenetic regulation skeletal development, incorporating evolutionary, genomic, epigenomic, multi-omics insights. We focus architecture causative or candidate genes traits, as revealed by genome-wide association studies (GWAS) selective sweep signatures, underscoring their adaptive significance potential breeding. role mechanisms, such DNA methylation, histone modifications, non-coding RNAs, linking variation is also discussed. By synthesizing multi-omic data, we a comprehensive understanding networks driving growth differentiation. aims consolidate current knowledge offer actionable insights advance research, applications biomedical fields.

Language: Английский

Citations

0