Combined analysis of 16S rRNA gene sequencing data reveals core vaginal bacteria across livestock species DOI Creative Commons
Lucille C. Jonas, Curtis R. Youngs, Stephan Schmitz‐Esser

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 10, 2025

Investigating the livestock vaginal microbiota is of increasing interest due to its relationship with animal reproductive performance. Recent publications have uncovered a high degree variability microbiota, making it difficult focus functional research on individual microorganisms. To address this variability, we conducted combined analysis publicly available 16S rRNA gene amplicon sequencing datasets reveal core in cattle, sheep, and pigs. The goal was identify bacterial genera that were shared despite diverse overall sample population. A total 2,911 samples (715 964 1,232 pigs) from 29 different used analysis. Beta diversity revealed structural differences between species. Compositionally, most abundant phyla Bacillota , Pseudomonadota Bacteroidota. At genus level, an unclassified Pasteurellaceae genus, Ureaplasma Streptococcus abundant. Across species, compositional observed. cattle sheep contained higher abundance Histophilus whereas pig more Fusobacterium Parvimonas than other samples. Among 120 OTUs 82 present 70% same threshold, had 40 63 genera, while 22 50 genera. There 19 overlapping across three largely species-specific, although there eight OTUs. These included (OTU 21), Clostridium sensu stricto 1 18), Corynebacterium 6), which also some members microbiota. better understanding required for future studies aimed at elucidation significance microbes respect efficiency. identified will help guide mechanisms/pathways through organisms enhance or impede

Language: Английский

Combined analysis of 16S rRNA gene sequencing data reveals core vaginal bacteria across livestock species DOI Creative Commons
Lucille C. Jonas, Curtis R. Youngs, Stephan Schmitz‐Esser

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 10, 2025

Investigating the livestock vaginal microbiota is of increasing interest due to its relationship with animal reproductive performance. Recent publications have uncovered a high degree variability microbiota, making it difficult focus functional research on individual microorganisms. To address this variability, we conducted combined analysis publicly available 16S rRNA gene amplicon sequencing datasets reveal core in cattle, sheep, and pigs. The goal was identify bacterial genera that were shared despite diverse overall sample population. A total 2,911 samples (715 964 1,232 pigs) from 29 different used analysis. Beta diversity revealed structural differences between species. Compositionally, most abundant phyla Bacillota , Pseudomonadota Bacteroidota. At genus level, an unclassified Pasteurellaceae genus, Ureaplasma Streptococcus abundant. Across species, compositional observed. cattle sheep contained higher abundance Histophilus whereas pig more Fusobacterium Parvimonas than other samples. Among 120 OTUs 82 present 70% same threshold, had 40 63 genera, while 22 50 genera. There 19 overlapping across three largely species-specific, although there eight OTUs. These included (OTU 21), Clostridium sensu stricto 1 18), Corynebacterium 6), which also some members microbiota. better understanding required for future studies aimed at elucidation significance microbes respect efficiency. identified will help guide mechanisms/pathways through organisms enhance or impede

Language: Английский

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