Evaluating microcystinase A-based approach on microcystins degradation during harvested cyanobacterial blooms

Environmental Pollution, Journal Year: 2024, Volume and Issue: 348, P. 123878 - 123878

Published: March 26, 2024

Language: Английский

---

Insights into cyanobacterial blooms through the lens of omics

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 934, P. 173028 - 173028

Published: May 7, 2024

Cyanobacteria are oxygen-producing photosynthetic bacteria that convert carbon dioxide into biomass upon exposure to sunlight. However, favorable conditions cause harmful cyanobacterial blooms (HCBs), which the dense accumulation of at water surface or subsurface, posing threats freshwater ecosystems and human health. Understanding mechanisms underlying bloom formation is crucial for effective management. In this regard, recent advancements in omics technologies have provided valuable insights HCBs, raised expectations develop more control methods near future. This systematic literature review aims present genomic architecture, adaptive mechanisms, microbial interactions, ecological impacts HCBs through lens omics. Genomic analysis indicates genome plasticity cyanobacteria has enabled their resilience adaptation environmental changes. Transcriptomic investigations revealed use various strategies adapting stress. Additionally, metagenomic metatranscriptomic analyses emphasized significant role community regulating HCBs. Finally, we offer perspectives on potential opportunities further research field.

Language: Английский

---

Taxonomic and functional metagenomic assessment of a dolichospermum bloom in a large and deep lake south of the Alps

FEMS Microbiology Ecology, Journal Year: 2024, Volume and Issue: 100(10)

Published: Sept. 3, 2024

Abstract Untargeted genetic approaches can be used to explore the high metabolic versatility of cyanobacteria. In this context, a comprehensive metagenomic shotgun analysis was performed on population Dolichospermum lemmermannii collected during surface bloom in Lake Garda summer 2020. Using phylogenomic approach, almost complete metagenome-assembled genome obtained from allowed clarify taxonomic position species within genus and contributed frame taxonomy ADA group (Anabaena/Dolichospermum/Aphanizomenon). addition common functional traits represented central metabolism photosynthetic cyanobacteria, annotation uncovered some distinctive adaptive that helped define factors promote maintain bloom-forming heterocytous nitrogen-fixing Nostocales oligotrophic lakes. addition, clusters were identified potentially encode several secondary metabolites previously unknown populations evolving southern Alpine district. These included geosmin, anabaenopetins, other bioactive compounds. The results expanded knowledge competitive drive algal blooms provided guidance for more targeted analyses cyanobacterial with implications human health water resource use.

Language: Английский

---

Contrasting but interconnecting metatranscriptome between large buoyant and small suspended particles during cyanobacterial blooming in the large shallow eutrophic Taihu Lake

Water Research, Journal Year: 2024, Volume and Issue: 267, P. 122539 - 122539

Published: Sept. 28, 2024

Language: Английский

---

Using a separation method to study the intra-colony cellular response in cyanobacterium Microcystis

Journal of Applied Phycology, Journal Year: 2024, Volume and Issue: 36(3), P. 1209 - 1218

Published: Jan. 3, 2024

Language: Английский

---

Diversity and networking of uni-cyanobacterial cultures and associated heterotrophic bacteria from the benthic microbial mat of a desert hydrothermal spring

FEMS Microbiology Ecology, Journal Year: 2024, Volume and Issue: 100(12)

Published: Nov. 18, 2024

Abstract Thermal springs harbour microorganisms, often dominated by cyanobacteria, which form biofilms and microbial mats. These phototrophic organisms release organic exudates into their immediate surroundings, attracting heterotrophic bacteria that contribute to the diversity functioning of these ecosystems. In this study, mats from a hydrothermal pool in Ksar Ghilane oasis Grand Erg Oriental Desert Tunisia were collected obtain cyanobacterial cultures formed single species. High-throughput analysis showed while mat hosted diverse laboratory selectively enriched cyanobacteria Leptolyngbya, Nodosilinea, Arthronema. Per each genera, multiple non-axenic uni-cyanobacterial established, totalling 41 cultures. Cyanobacteria taxa mediated assembly distinct bacterial communities, with members Proteobacteria Actinobacteria phyla dominating. The communities densely interconnected, preferentially co-occurring other. Our study highlighted complex structures cultures, where taxonomically consistently associate specific groups bacteria. observed associations likely driven common selection pressures laboratory, such as cultivation conditions hosts, may not necessarily reflect dynamic occurring spring

Language: Английский

---

Response Mechanisms of Phaeodactylum Tricornutum to 2,6-Di-Tert-Butyl-P-Cresol (Bht) Stress: Implications of Indole-3-Acetic Acid (Iaa) in Microalgae-Bacteria Interactions

Published: Jan. 1, 2024

2, 6-di-tert-butyl-p-cresol (BHT) is a synthetic phenolic antioxidant and pose potential threat to aquatic organisms. However, as the emerging contaminant, little known about response of microalgae BHT. In this study, BHT significantly inhibited growth photosynthetic pigment content Phaeodactylum tricornutum with increase concentration. The exposure also led an in malondialdehyde algal cells, indicating oxidative stress caused by Simultaneously, responded environment enhancing activities three enzymes, superoxide dismutase, catalase peroxidase eliminate excess reactive oxygen species cells during various time. Furthermore, transcriptome analysis revealed that up-regulation genes related photosynthesis, TCA cycle, phosphorylation indole-3-acetic acid (IAA) stress. addition, our high-throughput Illumina MiSeq sequencing results demonstrated significant relative abundance BHT-tolerant bacteria affiliated Halomonas, which was helpful reduce on P. tricornutum. Microbiological assays further Halomonas can thrive using IAA sole energy source exhibit chemotactic IAA. Therefore, we conclude phycosphere promotes colonization or recruitment thereby adaptability environmental pressures. Overall, study provides comprehensive understanding physiological biochemical effects microalgae, highlight functional significance establishing interaction between algae-associated adverse environments.

Language: Английский

---

A summer in the Greater Paris: trophic status of peri-urban lakes shapes prokaryotic community structure and functional potential

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 1, 2024

Abstract With more than 12 million inhabitants, the Greater Paris offers a “natural laboratory” to explore effects of eutrophication on freshwater lakes within relatively restricted area. Here, four-month time-series was carried out during summertime monitor planktonic microbial communities nine located ∼70 km radius around (Île-de-France, France) comparable morphologies, yet distinct trophic statuses (mesotrophic hypereutrophic). The contribution status and intra-summer variations were investigated prokaryotic community structures (16S rRNA gene sequencing) functions (shotgun metagenomics). Sampled harbored highly diverse communities. Although their pool quite stable shared among lakes, taxonomical functional changes correlated. appears as main driver both structure potential. When focusing function involved in biogeochemical cycles, responses phosphorus limitation (mostly polyphosphate-related processes) highlighted between statuses. Hypereutrophic displayed highest contrast heterogeneity over time, suggesting regime shift compared lower status. This study explores influence lakes’ microbiome ecology, by comparing for first time potential close vicinity summer season.

Language: Английский

---

Synergistic Response of Phycosphere Bacteria with Microalga Phaeodactylum tricornutum under the 2,6-Di-tert-butyl-p-cresol Stress

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

The emerging contaminant 2, 6-di-tert-butyl-p-cresol (BHT) is a kind of synthetic phenolic antioxidant and can pose negative effects on the microalgal cells. Enriching specific bacteria from phycosphere environment was an important adaptive strategy for algae cells when they are under adverse stress. However, mechanism coordinating with microalgae in response to BHT stress remains poorly understood. Herein, effect Phaeodactylum tricornutum comprehensively analyzed. Results showed that growth photosynthetic pigment biosynthesis P. inhibited increase concentration. also led content malondialdehyde, therefore responded oxidative by enhancing activities enzymes, including superoxide dismutase, catalase peroxidase, eliminate excess reactive oxygen species Furthermore, transcriptome analysis revealed up-regulation genes related photosynthesis, TCA cycle, phosphorylation indole-3-acetic acid (IAA) In addition, high-throughput Illumina MiSeq sequencing results demonstrated significant relative abundance affiliated Halomonas, Marivita Oceanicaulis. Microbiological assays Halomonas thrive using as sole energy source exhibit chemotactic IAA. Therefore, we conclude IAA secreted promoted enrichment BHT-tolerant bacterium Halomonas, thereby it helpful environmental pressures adaptability tricornutum. Overall, this study provided comprehensive understanding physiological biochemical microalgae, highlight potential functional significance establishing interaction between algae-associated environments.

Language: Английский

---

Nutrient limitation determines biological interactions between a mixotrophic Chrysophyte and toxin-producing Microcystis

Journal of Plankton Research, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Abstract Blooms of toxigenic cyanobacteria pose a mounting risk to aquatic ecosystems. Relative abiotic drivers success, biotic processes have received less attention. Mixotrophic nanoflagellates that combine heterotrophic ingestion particulate prey with photoautotrophy are among the eukaryotes can resist cyanotoxins. We used laboratory experiments in culture integrate top-down (herbivory) and bottom-up (nitrogen phosphorus limitation) controls on growth production microcystin-LR, including biodegradation products, Microcystis by Ochromonas (mixotroph) Spumella (heterotroph). A notable reduction rate toxic was evident co-culture either or under P-limitation. Under P-limitation, also led concentration microcystin-LR (MC-LR an increase products. Grazing rates up 31 50 cell−1 day−1 were recorded for Spumella, respectively. The highest grazing observed N-limitation. Hence, it is likely herbivore N-limitation competitor nutrients Collectively, these results suggest role eukaryotic decreasing biomass toxicity associated blooms vary nutrient availability.

Language: Английский

---