Pre-sowing recurrent inoculation with Pseudomonas fluorescens promotes maize growth DOI Creative Commons
Marine Papin, Amélie Polrot,

Marie-Christine Breuil

et al.

Biology and Fertility of Soils, Journal Year: 2024, Volume and Issue: 61(1), P. 125 - 140

Published: Oct. 23, 2024

Despite the rapid development of microbial inoculants use, their effectiveness still lacks robustness, partly due to our limited understanding factors influencing establishment in soil. Recurrent inoculation can temporarily increase abundance, but effect this strategy on plant growth and resident community is poorly studied. Here, we investigated maize soil bacterial responses under recurrent plant-beneficial bacterium Pseudomonas fluorescens B177. We further assessed how was modulated by inoculant dose, application timing type. at high dose transiently increased abundance P. B177 resulted larger shifts compared a single event. Moreover, prior sowing had strongest growth, with shoot dry weight 47.4%, likely an indirect through early changes community. Altogether these findings highlight significance pre-sowing inoculations as alternative for promoting growth.

Language: Английский

Niche availability and competitive loss by facilitation control proliferation of bacterial strains intended for soil microbiome interventions DOI Creative Commons
Senka Čaušević, Manupriyam Dubey, Marian Morales

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 22, 2024

Abstract Microbiome engineering – the targeted manipulation of microbial communities is considered a promising strategy to restore ecosystems, but experimental support and mechanistic understanding are required. Here, we show that bacterial inoculants for soil microbiome may fail establish because they inadvertently facilitate growth native resident microbiomes. By generating microcosms in presence or absence standardized communities, how different nutrient availabilities limit outgrowth focal (three Pseudomonads), this might be improved by adding an artificial, inoculant-selective niche. Through random paired interaction assays agarose micro-beads, demonstrate that, addition direct competition, lose competitiveness facilitating bacteria. Metatranscriptomics experiments with toluene as selective niche inoculant Pseudomonas veronii indicate facilitation due loss uptake excreted metabolites taxa. Generation niches help favor their proliferation duration intended action while limiting competitive loss.

Language: Английский

Citations

15
Arbuscular mycorrhizal fungal interactions bridge the support of root‐associated microbiota for slope multifunctionality in an erosion‐prone ecosystem DOI Creative Commons
Tianyi Qiu, Josep Peñuelas, Yinglong Chen

et al.

iMeta, Journal Year: 2024, Volume and Issue: 3(3)

Published: March 25, 2024

Abstract The role of diverse soil microbiota in restoring erosion‐induced degraded lands is well recognized. Yet, the facilitative interactions among symbiotic arbuscular mycorrhizal (AM) fungi, rhizobia, and heterotrophic bacteria, which underpin multiple functions eroded ecosystems, remain unclear. Here, we utilized quantitative profiling ecological network analyses to explore interplay between diversity biotic associations root‐associated multifunctionality across an slope a Robinia pseudoacacia plantation on Loess Plateau. We found explicit variations different positions, associated with shifts limiting resources, including phosphorus (P) moisture. To cope P limitation, AM fungi were recruited by R. , assuming pivotal roles as keystones connectors within cross‐kingdom networks. Furthermore, facilitated assembly composition bacterial rhizobial communities, collectively driving multifunctionality. association rhizobia promoted through enhanced decomposition recalcitrant compounds, improved mineralization potential, optimized microbial metabolism. Overall, our findings highlight crucial fungal‐centered functional delivery landscapes, providing valuable insights for sustainable restoration ecosystems erosion‐prone regions.

Language: Английский

Citations

11
Dual role of multistage inoculation in manipulating bacterial community assembly patterns to improve degradation of lignocellulose during rice or corn straw composting DOI
Da Liu, Ziwei Jiang,

Wenxuan Feng

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: 505, P. 159283 - 159283

Published: Jan. 9, 2025

Language: Английский

Citations

1
Distinct seasonal and annual variability of prokaryotes, fungi and protists in cropland soil under different tillage systems and soil texture DOI Creative Commons
Haotian Wang, Jing‐Jing Yang, Damien Finn

et al.

Soil Biology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109732 - 109732

Published: Jan. 1, 2025

Language: Английский

Citations

1
Role of Bacillus subtilis exopolymeric genes in modulating rhizosphere microbiome assembly DOI Creative Commons
Caroline Sayuri Nishisaka,

João Paulo Ventura,

Harsh P. Bais

et al.

Environmental Microbiome, Journal Year: 2024, Volume and Issue: 19(1)

Published: May 14, 2024

Abstract Background Bacillus subtilis is well known for promoting plant growth and reducing abiotic biotic stresses. Mutant gene-defective models can be created to understand important traits associated with rhizosphere fitness. This study aimed analyze the role of exopolymeric genes in modulating tomato microbiome assembly under a gradient soil diversities using B. wild-type strain UD1022 its corresponding mutant eps−TasA , which defective exopolysaccharide ( EPS ) TasA protein production. Results qPCR revealed that eps−TasA− has diminished capacity colonize roots soils diluted microbial diversity. The analysis bacterial β-diversity significant differences fungal community structures following inoculation either or strains. Verrucomicrobiota Patescibacteria, Nitrospirota phyla were more enriched than inoculation. Co-occurrence when was inoculated tomato, exhibited lower level modularity, fewer nodes, communities compared . Conclusion advances our understanding genes, are not only root colonization but also play shaping assembly. Future research should concentrate on specific genetic their implications colonization, coupled modulation. These efforts will crucial optimizing PGPR-based approaches agriculture.

Language: Английский

Citations

6
Unravelling the secondary metabolome and biocontrol potential of the recently described species Bacillus nakamurai DOI Creative Commons

François Nimbeshaho,

Gaspard Nihorimbere,

Anthony Argüelles Arias

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 288, P. 127841 - 127841

Published: Aug. 2, 2024

In the prospect of novel potential biocontrol agents, a new strain BDI-IS1 belonging to recently described Bacillus nakamurai was selected for its strong in vitro antimicrobial activities against range bacterial and fungal phytopathogens. Genome mining coupled with metabolomics revealed that produces multiple non-ribosomal secondary metabolites including surfactin, iturin A, bacillaene, bacillibactin bacilysin, together some ribosomally-synthesized post-translationally modified peptides (RiPPs) such as plantazolicin, potentially amylocyclicin, bacinapeptin LCI. Reverse genetics further showed specific involvement these compounds antagonistic activity strain. Comparative genomics between five already sequenced B. strains products constitute core metabolome species while RiPPs are more strain-specific. Although lacks key bioactive found velezensis, greenhouse experiments show is able protect tomato maize plants early blight northern leaf caused by Alternaria solani Exserohilum turcicum, respectively, at levels similar or better than velezensis QST713. The reduction foliar diseases, following root application suspension demonstrates can act direct antibiosis inducing plant defence mechanisms. These findings indicate be considered good candidate diseases prevailing tropical regions, encourage research into spectrum activity, requirements conditions needed ensure efficacy.

Language: Английский

Citations

6
Natural plant disease suppressiveness in soils extends to insect pest control DOI Creative Commons
Nadine Harmsen, Pilar Vesga, Gaétan Glauser

et al.

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: July 16, 2024

Since the 1980s, soils in a 22-km2 area near Lake Neuchâtel Switzerland have been recognized for their innate ability to suppress black root rot plant disease caused by fungal pathogen Thielaviopsis basicola. However, efficacy of natural suppressive against insect pests has not studied. We demonstrate that soil suppressiveness also protects plants from leaf-feeding pest Oulema melanopus. Plants grown most reduced stress response feeding, reflected dampened levels herbivore defense-related phytohormones and benzoxazinoids. Enhanced salicylate insect-free indicate defense-priming operating this soil. The rhizosphere microbiome contained higher proportion plant-beneficial bacteria, coinciding with networks being highly tolerant destabilizing impact exposure observed conducive soils. suggest presence bacteria along priming, conferred resistance pest, manifesting onset dysbiosis displacement endosymbionts. Our results show an intricate soil-plant-insect feedback, relying on network extends soilborne diseases pests. Video Abstract.

Language: Английский

Citations

5
Expanding the Pseudomonas diversity of the wheat rhizosphere: four novel species antagonizing fungal phytopathogens and with plant-beneficial properties DOI Creative Commons

Noémie Poli,

Christoph Keel, Daniel Garrido‐Sanz

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: July 15, 2024

Plant-beneficial Pseudomonas bacteria hold the potential to be used as inoculants in agriculture promote plant growth and health through various mechanisms. The discovery of new strains tailored specific agricultural needs remains an open area research. In this study, we report isolation characterization four novel species associated with wheat rhizosphere. Comparative genomic analysis all available type revealed species-level differences, substantiated by both digital DNA-DNA hybridization average nucleotide identity, underscoring their status species. This was further validated phenotypic differences observed when compared closest relatives. Three belong P. fluorescens complex, two representing a lineage phylogeny. Functional genome annotation presence features contributing rhizosphere colonization, including flagella components for biofilm formation. have genetic solubilize nutrients acidifying environment, releasing alkaline phosphatases metabolism nitrogen species, indicating biofertilizers. Additionally, possess traits that may facilitate direct promotion modulation hormone balance, ACC deaminase enzyme auxin metabolism. biosynthetic clusters toxins such hydrogen cyanide non-ribosomal peptides suggests ability compete other microorganisms, pathogens. Direct inoculation roots significantly enhanced growth, doubling shoot biomass. effectively antagonized fungal phytopathogens (Thielaviopsis basicola, Fusarium oxysporum, Botrytis cinerea), demonstrating biocontrol agents. Based on from closely related propose following names species: grandcourensis sp. nov., strain DGS24T ( = DSM 117501T CECT 31011T), purpurea DGS26T 117502T 31012T), helvetica DGS28T 117503T 31013T) aestiva DGS32T 117504T 31014T).

Language: Английский

Citations

5
Planting halophytes increases the rhizosphere ecosystem multifunctionality via reducing soil salinity DOI
Jinpeng Hu, Yuan-Yuan He, Jianhong Li

et al.

Environmental Research, Journal Year: 2024, Volume and Issue: 261, P. 119707 - 119707

Published: July 30, 2024

Language: Английский

Citations

5
Plant growth promoting endophyte modulates soil ecological characteristics during the enhancement process of cadmium phytoremediation DOI
Yaowei Chi,

Xianzhong Ma,

Xia Zhang

et al.

Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 369, P. 122206 - 122206

Published: Aug. 27, 2024

Language: Английский

Citations

5