Fecal microbiota transplants facilitate post-antibiotic recovery of gut microbiota in cheetahs (Acinonyx jubatus) DOI Creative Commons
Sally L. Bornbusch, Adrienne E. Crosier, Lindsey K Gentry

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: Dec. 23, 2024

Burgeoning study of host-associated microbiomes has accelerated the development microbial therapies, including fecal microbiota transplants (FMTs). FMTs provide host-specific supplementation, with applicability across host species. Studying can simultaneously comparative frameworks for understanding therapies in diverse systems and improve health managed wildlife. Ex-situ carnivores, cheetahs (Acinonyx jubatus), often suffer from intractable gut infections similar to those treated antibiotics humans, providing a valuable system testing FMT efficacy. Using an experimental approach 21 cheetahs, we tested whether autologous facilitated post-antibiotic recovery microbiota. We used 16S rRNA sequencing source tracking characterize antibiotic-induced extirpations signatures engraftment single versus multiple FMTs. found that extirpated abundant bacteria quickened via may facilitate protein digestion butyrate production (Fusobacterium). Although better sustained compared FMT, one improved alone. This elucidated dynamics microbiome modulation non-model improves foundations reproducible, low-cost, low-dose, minimally invasive protocols, emphasizing scientific applied value Autologous recouping composition engrafting specific taxa. elucidate host-microbe animal care.

Language: Английский

Decoding the intestinal response to heat stress in Gymnocypris eckloni: Insights from a thorough analysis of microbiome and transcriptome DOI
Chaowei Zhou,

Yuting Duan,

Junting Li

et al.

Aquaculture, Journal Year: 2024, Volume and Issue: 591, P. 741112 - 741112

Published: May 21, 2024

Language: Английский

Citations

4
All roads lead to Rome: the plasticity of gut microbiome drives the extensive adaptation of the Yarkand toad-headed agama (Phrynocephalus axillaris) to different altitudes DOI Creative Commons

Jiang‐hao Du,

Peng Zheng, Weizhen Gao

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 8, 2025

The gut microbiome was involved in a variety of physiological processes and played key role host environmental adaptation. However, the mechanisms their response to altitudinal changes remain unclear. In this study, we used 16S rRNA sequencing LC-MS metabolomics investigate metabolism Yarkand toad-headed agama (Phrynocephalus axillaris) at different altitudes (-80 m 2000 m). results demonstrated that Firmicutes, Bacteroidetes, Proteobacteria were dominant phylum, Lachnospiraceae Oscillospiraceae most abundant family, low-altitude populations had higher richness than high-altitude populations; Akkermansiaceae appeared be enriched relative abundance tended increase with altitude. three P. axillaris clustered into two enterotypes, shared an enterotype dominated by Akkermansia, Kineothrix, Phocaeicola; intermediate-altitude Mesorhizobium, Bradyrhizobium. Metabolites amino acid lipid differed significantly altitudes. above suggest plasticity drives extensive adaptation multi-stress caused With global warming, recognizing adaptive capacity wide-ranging species altitude can help plan future conservation strategies.

Language: Английский

Citations

0
Ontogenetic Analysis of Chelonus formosanus and Diversity of Its Internal Microbiota DOI Creative Commons
Jingjing Jia,

Qing Feng,

Wenhua Huang

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(2), P. 180 - 180

Published: Feb. 8, 2025

Chelonus formosanus is a parasitic wasp capable of parasitizing various Noctuidae pests, including the highly invasive Spodoptera frugiperda, and it demonstrates strong pest control potential. Both egg larval stages primarily occur within host organism, total developmental time from to adult approximately 19.62 days. To investigate microbial communities at different stages, we performed 16S rDNA sequencing (V1–V9 region) using PacBio identified 404 bacterial species belonging 61 classes, 116 orders, 182 families, 308 genera across larval, pupal, female, male stages. Bacterial diversity richness varied with Enterobacter Enterococcus dominating in larvae pupae Pseudomonas emerging as dominant female adults. In contrast, adults predominantly resided Ralstonia Achromobacter. The predicted functions bacteria C. are marked by high abundance metabolic pathways. This study provides comprehensive understanding morphology contributes practical pests. Additionally, our findings preliminarily characterized community laying groundwork for its functional study.

Language: Английский

Citations

0
Characteristics and dynamic changes of gut microbiota in Mongolian horses and Guizhou horses DOI Creative Commons

Yaonan Li,

Yanfang Lan

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 15, 2025

The gut microbial importance and its crucial roles in host digestion, immunity, metabolism have received widely attention. Horses, especially indigenous varieties such as Mongolian horses (MGH) Guizhou (GZH), not sufficient attention, the characteristics of their microbiota are still unclear. For this purpose, we collected faecal samples from eight MGH GZH to compare differences using amplicon sequencing. results alpha diversity analysis indicated that bacterial fungal abundance were significantly higher than those MGH. Meanwhile, beta revealed there significant structures between Although dominant phyla same, a large number different bacteria fungi both groups. Moreover, observed 32 (23 9 phyla) 718 genera (383 335 genera) with Notably, study also some intestinal functions GZH, chemoheterotrophy, fermentation, cellulolysis. To our knowledge, is first report on comparative GZH. Our demonstrated richer more diverse compared Additionally, these important for understanding horse.

Language: Английский

Citations

0
Consumption of centipedes promotes growth and gut microbiome homeostasis in a lizard DOI Creative Commons
Hongxin Xie, Jing Yang,

Guo-Shuai Tang

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 1(1), P. 29 - 39

Published: March 1, 2024

Abstract Animals are known for their self‐medicating behavior, and food composition also contributes to animal fitness. In this study, we investigated the dietary habits of endangered Chinese crocodile lizards found that, compared adult lizards, juvenile consumed more centipedes, a trend validated through controlled feeding experiments. Juvenile fed with centipedes demonstrated faster growth rates better body conditions than those an earthworm‐only diet, despite no increase in intake. The centipede‐added group showed lower species richness gut microbiome had abundant fermentative bacteria, such as Bacteroides , Paraclostridium Enterococcus whereas non‐fermentative opportunistic pathogens. Higher concentrations short‐chain fatty acids, which mainly derived from bacterial fermentation gut, were feces group. Therefore, consumption promoted microbiota homeostasis lizards. This study emphasizes significance preserving diversity natural environments increasing captive populations.

Language: Английский

Citations

1
Unlocking the power of short-chain fatty acids in ameliorating intestinal mucosal immunity: a new porcine nutritional approach DOI Creative Commons

Haoyang Liu,

Hongde Lu,

Yuxuan Wang

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Sept. 2, 2024

Short-chain fatty acids (SCFAs), a subset of organic with carbon chains ranging from one to six atoms in length, encompass acetate, propionate, and butyrate. These compounds are the endproducts dietary fiber fermentation, primarily catalyzed by glycolysis pentose phosphate pathways within gut microbiota. SCFAs act as pivotal energy substrates signaling molecules realm animal nutrition, exerting profound influence on intestinal, immune system, intestinal barrier functions. Specifically, they contibute 60-70% total requirements ruminants 10-25% monogastric animals. have demonstrated capability effectively modulate pH, optimize absorption mineral elements, impede pathogen invasion. Moreover, enhance expression proteins associated tight junctions stimulate mucus production, thereby refining tissue morphology preserving integrity structure. Notably, also exert anti-inflammatory properties, mitigating inflammation epithelium strengthening barrier’s defensive capabilities. The present review endeavors synthesize recent findings regarding role crucial intermediaries between metabolic activities microbiota status porcine cells. It provides comprehensive overview current literature SCFAs’ impact responses mucosa.

Language: Английский

Citations

1
Differences in Gut Microbial Composition and Characteristics Among Three Populations of the Bamboo Pitviper (Viridovipera stejnegeri) DOI Creative Commons
Jiaqi Zhang,

Songwen Tan,

Bing Lyu

et al.

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(12)

Published: Dec. 1, 2024

ABSTRACT The gut microbiota contributes to host health by facilitating nutrient uptake, digestion, energy metabolism, intestinal development, vitamin synthesis, and immunomodulation, plays an important role in the growth reproduction of animal itself. Considering paucity research on wild snakes, this study focused bamboo pitviper ( Viridovipera stejnegeri ) populations from Anhui, Guizhou, Hunan, with multiple fecal samples collected each population (six, five, three, respectively). Total microbial DNA was extracted using metagenomic next‐generation sequencing differences composition, abundance, carbohydrate‐active enzymes (CAZymes) were analyzed compared among three populations. Results showed no significant variance α‐diversity microbes across populations, while principal coordinate analysis revealed microbe composition. four most abundant phyla V. Pseudomonadota, Bacteroidota, Actinomycetota, Bacillota, genera Salmonella , Citrobacter Bacteroides Yokenella . Linear discriminant effect size demonstrated notable abundance Marked CAZyme also observed communities. Future studies should incorporate diverse ecological factors evaluate their influence composition function microbiota.

Language: Английский

Citations

1
Fecal microbiota transplants facilitate post-antibiotic recovery of gut microbiota in cheetahs (Acinonyx jubatus) DOI Creative Commons
Sally L. Bornbusch, Adrienne E. Crosier, Lindsey K Gentry

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: Dec. 23, 2024

Burgeoning study of host-associated microbiomes has accelerated the development microbial therapies, including fecal microbiota transplants (FMTs). FMTs provide host-specific supplementation, with applicability across host species. Studying can simultaneously comparative frameworks for understanding therapies in diverse systems and improve health managed wildlife. Ex-situ carnivores, cheetahs (Acinonyx jubatus), often suffer from intractable gut infections similar to those treated antibiotics humans, providing a valuable system testing FMT efficacy. Using an experimental approach 21 cheetahs, we tested whether autologous facilitated post-antibiotic recovery microbiota. We used 16S rRNA sequencing source tracking characterize antibiotic-induced extirpations signatures engraftment single versus multiple FMTs. found that extirpated abundant bacteria quickened via may facilitate protein digestion butyrate production (Fusobacterium). Although better sustained compared FMT, one improved alone. This elucidated dynamics microbiome modulation non-model improves foundations reproducible, low-cost, low-dose, minimally invasive protocols, emphasizing scientific applied value Autologous recouping composition engrafting specific taxa. elucidate host-microbe animal care.

Language: Английский

Citations

0