Garlic-Derived Exosome-Like Nanoparticles Enhance Gut Homeostasis in Stressed Piglets: Involvement of Lactobacillus reuteri Modulation and Indole-3-propionic Acid Induction DOI
Xiyuan Liu,

Xinyu Zhang,

Haozhen Liu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

The occurrence of pediatric diarrhea is frequently associated with inflammatory responses, compromised barrier function, and dysbiosis in the gut. These conditions are commonly triggered by stressors, similar to postweaning observed piglets. Garlic-derived exosome-like nanoparticles (GELNs) hold potential for ameliorating stress-induced diarrhea, yet supporting evidence remains scarce. Following successful isolation GELNs, this study employed weaned piglets as a model evaluate regulatory effects GELNs on intestinal integrity, mucosal inflammation, gut microbiota its metabolites. Weaned Bama miniature were orally administered phosphate buffer saline (PBS) or 1 week later, samples collected following slaughter. Histological molecular biological techniques performed examine structure, tight junction protein expression, mucin secretion, T lymphocyte infiltration, levels pro-inflammatory cytokines. composition was analyzed using 16S rRNA sequencing, while derived metabolites profiled via untargeted metabolomics. Subsequently, correlation analyses associations between metabolites, well key indicators function cytokine response GELNs. isolated exhibit typical exosome characteristics size morphology, alongside rich content proteins RNAs. incidence reduced supplementation at dosage 50 mg/kg body weight, compared control group. In addition, receiving displayed an increase within tissues jejunum, ileum, colon, decrease CD8+ counts suppression cytokines (IL-8 TNF-α) layers both jejunum ileum. Furthermore, sequencing unveiled that reshaped colonic augmenting beneficial bacteria, notably Lactobacillus reuteri, correlating strongly diminished TNF-α heightened expression. Metabolite analysis demonstrated significant indole-3-propionic acid, from microbiota, supplemented This positively correlated abundance reuteri negatively linked IL-8 summary, our demonstrates mitigate stress-related inflammation enhance production piglets, which potentially achieved through optimization composition, specifically increasing induction anti-inflammatory microbial metabolite acid. findings presented here provide essential groundwork future development therapeutic strategy aimed enhancing homeostasis disruption caused stress children.

Language: Английский

Colorectal cancer in Lynch syndrome families: consequences of gene germline mutations and the gut microbiota DOI Creative Commons
Xuexin Wang,

Zhijun Zheng,

Dongliang Yu

et al.

Orphanet Journal of Rare Diseases, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 18, 2025

Lynch syndrome (LS)-associated colorectal cancer (CRC) always ascribes to pathogenic germline mutations in mismatch repair (MMR) genes. However, the penetrance of CRC varies among those with same MMR gene mutation. Thus, we hypothesized that gut microbiota is also involved development LS families. This prospective, observational study was performed from December 2020 March 2023. We enrolled 72 individuals 9 families across six provinces China and employed 16S rRNA amplicon sequencing analyze fecal components LS-related patients (AS group), their spouses (BS mutation carriers without (CS non-mutation (DS group) using alpha beta diversity indices. There were no apparent differences age or gender four groups. Alpha indices exhibited significant between AS BS groups, verifying role occurrence Beta analysis CS revealing importance for A greater difference (both indices) shown DS demonstrating combined impact genetic on Compared identified ten microbial genera enriched group, one genus (Bacteroides) decreased group. Among elevated Agathobacter, Coprococcus Prevotellaceae_NK3B31_group butyrate-producing genera. found can be attributed effects as well provided novel insights into prevention treatment

Language: Английский

Citations

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Metaproteomics and metabonomics reveal the metabolic dysfunction of gut microbiota in Tibetan Minipigs in Atherosclerosis DOI Creative Commons
Liye Shen, Jinlong Wang, Yongming Pan

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Abstract Atherosclerosis is fundamental in the development of cardiovascular disease. The unhealthy dietary habits, high fat and cholesterol intake could change composition gut microbes metabolites which play a critical role atherosclerosis. However, few studies have systematically investigated metabolism In this study, we build an atherosclerosis model using Tibetan minipigs, then identified feces serum, explored functions microbiota by metaproteomics. We found that, feces, multiple signal pathways showed obvious metabolic dysfunction that influence abundance blood products. Several such as 3-dehydro-2-deoxyecdysone from metabolism, leukotriene B4 arachidonic acid indole-3-acetate 3-hydroxyanthranilate tryptophan 9,10-epoxyoctadecenoic linoleic 13(S)-HPOT linolenic were significantly increased blood. These partially increasing associated with inflammation contributes Our finding provide novel clues for studying on mechanism arteriosclerosis.

Language: Английский

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Insights into the Metabolite Differentiation Mechanism Between Chinese Dry-Cured Fatty Ham and Lean Ham Through UPLC-MS/MS-Based Untargeted Metabolomics DOI Creative Commons

Ruoyu Xie,

Xiaoli Wu,

Jun Hu

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(3), P. 505 - 505

Published: Feb. 5, 2025

To understand the impact and mechanism of removing fat skin tissue on nutritional metabolism Chinese dry cured ham, differential metabolites (DMs) profile between lean ham (LH) fatty (FH) was explored though untargeted metabolomics based UPLC-MS/MS. The results showed significant differences metabolite profiles FH LH. A total 450 defined were detected, 266 among them had significantly different abundances two hams, mainly including organic acids derivatives, lipids lipid-like molecules, as well organoheterocyclic compounds. Furthermore, 131 identified DMs, which 101 30 DMs remarkably higher contents in LH, respectively. further Kyoto Encyclopedia Genes Genomes (KEGG) analysis suggested that can be mostly enriched pathways ABC transporters, amino acid biosynthesis, protein digestion absorption, aminoacyl-tRNA 2-oxocarboxylic metabolism. Moreover, metabolic network revealed prominent FH, such 9(S)-HODE, 9,10-EpOME, 13-Oxo-ODE, L-palmitoyl carnitine, D-fructose, primarily involved endogenous oxidation degradation glycogen. Nevertheless, dominant 2-isopropylmalic acid, indolelactic hydroxyisocaproic microbial derivates. These findings could help us how fat-deficiency affects dry-cured hams from a perspective.

Language: Английский

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Lactobacillus vaginalis alleviates DSS induced colitis by regulating the gut microbiota and increasing the production of 3-indoleacrylic acid DOI Creative Commons

Zhuoya Wang,

Liu Tian, Li Liu

et al.

Pharmacological Research, Journal Year: 2025, Volume and Issue: 213, P. 107663 - 107663

Published: Feb. 16, 2025

Language: Английский

Citations

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β-1,3-glucans from Euglena Gracilis protects against ulcerative colitis via modulating the gut barrier, T-cell immunity and gut microbiota DOI
Bingbing Guo, Weihao Zhang, Jingwen Zou

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 305, P. 141288 - 141288

Published: Feb. 20, 2025

Language: Английский

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Complementary Feeding and Infant Gut Microbiota: A Narrative Review DOI Open Access

Danielle L. Noles,

Kinzie L. Matzeller,

Daniel N. Frank

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(5), P. 743 - 743

Published: Feb. 20, 2025

Background: The complementary feeding period, spanning from 6 to 24 months of age, marks the transition an exclusive liquid diet in infants a dietary pattern requiring introduction solid foods meet nutritional demands. Complementary coincides with other critical development windows, including maturation gut microbiome. However, effects specific on microbiota and subsequent influence health outcomes require further investigation. Methods: This narrative review analyzes published research January 2004 October 2024 aims summarize current evidence infant microbiota. Results: A total 43 studies were included this review. Overall, multiple reported increase alpha-diversity after food introduction. Bifidobacteriaceae is predominant bacterial family during first life, shifting Lachnospiraceae, Ruminococcaceae, Clostridium spp. foods. timing may also microbiota, though results inconclusive. effect individual components was conflicting, limited make inferences. Conclusions: Because variations study design, intake quantification, minimal follow-up, lack conclusive exists describing relationship between infants. Future describe these relationships should focus impact microbial diversity maturation, as well outcomes.

Language: Английский

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Bifidobacterium longum Ameliorates Intestinal Inflammation and Metabolic Biomarkers in Mice Fed a High-Fat Diet with Gliadin by Indoleacrylic Acid DOI
Ning Wang, Zhangming Pei,

Hongchao Wang

et al.

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

Language: Английский

Citations

0
Circulating Interleukin-22 Is a Biomarker for Newly Diagnosed Type 2 Diabetes Mellitus and Associated with Hypoglycemic Effect of Sitagliptin DOI Creative Commons
Pinghui Sun,

Yuxi Xiao,

Yuan Dong

et al.

Diabetes Metabolic Syndrome and Obesity, Journal Year: 2025, Volume and Issue: Volume 18, P. 703 - 713

Published: March 1, 2025

Interleukin-22 (IL-22) has been demonstrated to be involved in the regulation of glucose metabolism, insulin resistance and inflammation response, which indicates that IL-22 might associated with occurrence progression diabetes. This study aimed assess serum levels participants type 2 diabetes mellitus (T2DM) analyze association between T2DM risk. Serum concentrations recruited healthy (n=48), newly diagnosed (n=46), receiving placebo (n=7) or dipeptidyl peptidase-4 inhibitors (DPP-4i) sitagliptin monotherapy were measured using a commercial enzyme-linked immunosorbent assay (ELISA) kit. Mice fed high-fat diet (HFD) administered evaluated for intestinal inflammation-related indicators. higher group (127.16 ± 75.35) than controls (69.18 32.83, p < 0.001), significantly negatively correlated high-density lipoprotein cholesterol (HDL-C), positively body mass index (BMI), glycosylated hemoglobin (HbA1c) fasting plasma (FPG), regardless adjustment sex age. Multivariate logistic regression analysis showed risk (OR = 2.37, 95% CI 1.27-4.42, 0.007). Additionally, treatment decreased colon tissues HFD mice. Moreover, was improved, retinoid acid-related orphan receptor γt (RORγt, marker Th17 cells)- positive cells mice after treatment, related reduction IL-22. is significant independent factor T2DM, implying circulating may predictive biomarker therapeutic target T2DM.

Language: Английский

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Advancements in the investigation of gut microbiota-based strategies for stroke prevention and treatment DOI Creative Commons
Min Wang, Yan Liu, Li Zhong

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 4, 2025

Stroke represents a predominant cause of mortality and disability on global scale, impacting millions annually exerting considerable strain healthcare systems. The incidence stroke exhibits regional variability, with ischemic accounting for the majority occurrences. Post-stroke complications, such as cognitive impairment, motor dysfunction, recurrent stroke, profoundly affect patients’ quality life. Recent advancements have elucidated microbiota-gut-brain axis (MGBA), underscoring complex interplay between gut health brain function. Dysbiosis, characterized by an imbalance in microbiota, is significantly linked to elevated risk unfavorable outcomes. MGBA plays crucial role modulating immune function, neurotransmitter levels, metabolic byproducts, which may intensify neuroinflammation impair cerebral health. This review elucidates pathophysiology explores potential gut-targeted therapeutic strategies reduce promote recovery, including probiotics, prebiotics, pharmacological interventions, dietary modifications. However, current prevention treatment based intestinal flora still face many problems, large difference individual flora, stability efficacy, long-term safety need be considered. Further research needs strengthened its better application clinical practice.

Language: Английский

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Decreased Gut microbiome-derived Indole-3-propionic Acid Mediates the Exacerbation of Myocardial Ischemia/Reperfusion Injury Following Depression via the Brain-gut-heart Axis DOI Creative Commons

Xingdou Mu,

Lele Feng,

Qiang Wang

et al.

Redox Biology, Journal Year: 2025, Volume and Issue: 81, P. 103580 - 103580

Published: March 5, 2025

Despite the increasing recognition of interplay between depression and cardiovascular disease (CVD), precise mechanisms by which contributes to pathogenesis remain inadequately understood. The involvement gut microbiota their metabolites health susceptibility has been gaining attention. In this study, it was found that exacerbated cardiac injury, impaired function (EF%: P < 0.01; FS%: 0.05), hindered long-term survival (P 0.01), intensified adverse remodeling (WGA: MASSON: 0.0001) after myocardial ischemia/reperfusion (MI/R) in mice. Then we mice receiving transplants from chronic social defeat stress (CSDS) exhibited worse 0.01) than those non-CSDS MI/R injury. Moreover, tryptophan metabolism due alterations composition structure observed CSDS Mechanistically, analyzed metabolomics fecal serum samples identified indole-3-propionic acid (IPA) as a protective agent for cardiomyocytes against ferroptosis via NRF2/System xc-/GPX4 axis, played role mediating detrimental influence on MI/R. Our findings provide new insights into IPA CVD, forming basis intervention strategies aimed at mitigating deterioration following patients experiencing depression.

Language: Английский

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