Genomes of Prochlorococcus, Synechococcus, bacteria, and viruses recovered from marine picocyanobacteria cultures based on Illumina and Qitan nanopore sequencing DOI Creative Commons
Qingtao Wu, Jie Gao,

Boxuan Sa

et al.

Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)

Published: April 12, 2025

Prochlorococcus and Synechococcus are key contributors to marine primary production play essential roles in global biogeochemical cycles. Despite the ecological importance of these two picocyanobacterial genera, current genomic datasets still lack comprehensive representation under-sampled ocean regions, associated bacteria viruses. To address this gap, we used a combination second- third-generation sequencing technologies assemble data from 105 Picocyanobacterial enrichment cultures isolated Indian Ocean, South China Sea, western Pacific Ocean. This dataset includes 55 50 genomes with high completeness (>98%) low contamination (<2%), along 308 non-redundant bacterial derived 1,457 medium- high-quality non-cyanobacteria metagenome-assembled (MAGs, ≥50% ≤10%). Additionally, 2,113 viral operational taxonomic units (vOTUs) were total 7632 qualified contigs. provides valuable resource for improving our understanding complex interactions among Prochlorococcus, Synechococcus, their viruses ecosystems, offering foundation study evolutionary dynamics.

Language: Английский

Cyanobacterial type I CRISPR-Cas systems: distribution, mechanisms, and genome editing applications DOI Creative Commons

Yongjiu Zhang,

Shuxiao Yang,

Zhaofeng Li

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 27, 2025

Cyanobacteria, renowned for their photosynthetic capabilities, serve as efficient microbial chassis capable of converting carbon dioxide into a spectrum bio-chemicals. However, conventional genetic manipulation strategies have proven incompatible with the precise and systematic modifications required in field cyanobacterial synthetic biology. Here, we present an in-depth analysis endogenous CRISPR-Cas systems within genomes, particular focus on Type I systems, which are most widely distributed. We provide comprehensive summary reported DNA defense mechanisms mediated by current applications genome editing. Furthermore, offer insights future these context editing, underscoring potential to revolutionize biology approaches.

Language: Английский

Citations

0
Genomes of Prochlorococcus, Synechococcus, bacteria, and viruses recovered from marine picocyanobacteria cultures based on Illumina and Qitan nanopore sequencing DOI Creative Commons
Qingtao Wu, Jie Gao,

Boxuan Sa

et al.

Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)

Published: April 12, 2025

Prochlorococcus and Synechococcus are key contributors to marine primary production play essential roles in global biogeochemical cycles. Despite the ecological importance of these two picocyanobacterial genera, current genomic datasets still lack comprehensive representation under-sampled ocean regions, associated bacteria viruses. To address this gap, we used a combination second- third-generation sequencing technologies assemble data from 105 Picocyanobacterial enrichment cultures isolated Indian Ocean, South China Sea, western Pacific Ocean. This dataset includes 55 50 genomes with high completeness (>98%) low contamination (<2%), along 308 non-redundant bacterial derived 1,457 medium- high-quality non-cyanobacteria metagenome-assembled (MAGs, ≥50% ≤10%). Additionally, 2,113 viral operational taxonomic units (vOTUs) were total 7632 qualified contigs. provides valuable resource for improving our understanding complex interactions among Prochlorococcus, Synechococcus, their viruses ecosystems, offering foundation study evolutionary dynamics.

Language: Английский

Citations

0