Metagenomic analysis reveals the diversity of the vaginal virome and its association with vaginitis DOI Creative Commons
Xiaoju Lü, Qiang Lu,

Rong Zhu

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: April 3, 2025

The human vaginal virome is an essential yet understudied component of the microbiome. Its diversity and potential contributions to health disease, particularly vaginitis, remain poorly understood. We conducted metagenomic sequencing on 24 pooled swab libraries collected from 267 women, including both healthy individuals those diagnosed with vaginitis. Viral community composition, indices (Shannon, Richness, Pielou), phylogenetic characteristics were analyzed. Virus-host associations also investigated. DNA viruses dominated virome. Anelloviridae Papillomaviridae most prevalent eukaryotic viruses, while Siphoviridae Microviridae leading bacteriophages. Compared controls, vaginitis group exhibited significantly reduced alpha greater beta dispersion, indicating altered viral structure. Anelloviruses, detected in groups, showed extensive lineage diversity, frequent recombination, pronounced divergence. HPV richness elevated group, alongside unbalanced distribution lineages. Novel phage-bacterial identified, suggesting a role for bacteriophages shaping These findings provide new insights into composition structure its association dysbiosis. distinct observed women highlight relevance communities reproductive health. Future studies incorporating individual-level metatranscriptomics are warranted explore intra-host dynamics, assess activity, clarify functional roles host-microbiome interactions.

Language: Английский

Metagenomic analysis reveals the diversity of the vaginal virome and its association with vaginitis DOI Creative Commons
Xiaoju Lü, Qiang Lu,

Rong Zhu

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: April 3, 2025

The human vaginal virome is an essential yet understudied component of the microbiome. Its diversity and potential contributions to health disease, particularly vaginitis, remain poorly understood. We conducted metagenomic sequencing on 24 pooled swab libraries collected from 267 women, including both healthy individuals those diagnosed with vaginitis. Viral community composition, indices (Shannon, Richness, Pielou), phylogenetic characteristics were analyzed. Virus-host associations also investigated. DNA viruses dominated virome. Anelloviridae Papillomaviridae most prevalent eukaryotic viruses, while Siphoviridae Microviridae leading bacteriophages. Compared controls, vaginitis group exhibited significantly reduced alpha greater beta dispersion, indicating altered viral structure. Anelloviruses, detected in groups, showed extensive lineage diversity, frequent recombination, pronounced divergence. HPV richness elevated group, alongside unbalanced distribution lineages. Novel phage-bacterial identified, suggesting a role for bacteriophages shaping These findings provide new insights into composition structure its association dysbiosis. distinct observed women highlight relevance communities reproductive health. Future studies incorporating individual-level metatranscriptomics are warranted explore intra-host dynamics, assess activity, clarify functional roles host-microbiome interactions.

Language: Английский

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