Genomic insight into COVID-19 severity in MAFLD patients: a single-center prospective cohort study

Frontiers in Genetics, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 4, 2024

This study investigated the influence of single nucleotide polymorphisms (SNPs) in genes associated with interferon pathway (IFNAR2 rs2236757), antiviral response (OAS1 rs10774671, OAS3 rs10735079), and viral entry (ACE2 rs2074192) on COVID-19 severity their association nonalcoholic fatty liver disease (MAFLD). We did not observe a significant between SNPs severity. While IFNAR2 rs2236757 A allele was correlated higher creatinine levels upon admission G lower band neutrophils discharge, these findings require further investigation. The distribution OAS gene (rs10774671 rs10735079) differ MAFLD patients non-MAFLD patients. Our population's ACE2 rs2074192 genotypes alleles differed from that European reference population. Overall, our suggest specific may be major contributors to patient population, highlighting potential role other genetic factors environmental influences.

Language: Английский

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The first assessment of Angiotensin-Converting Enzyme 1 (ACE1) D/I polymorphism and demographic factors in association with COVID-19 outcomes in the Moroccan Population

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: Jan. 8, 2025

Language: Английский

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The role of candidate genetic polymorphisms in covid-19 susceptibility and outcomes

BMC Medical Genomics, Journal Year: 2025, Volume and Issue: 18(1)

Published: Feb. 7, 2025

This study aims to investigate the association between candidate host genetic polymorphisms and COVID-19 susceptibility, severity, hospitalization, hypoxia, their combined effect, measured by polygenic risk score (PRS). Three hundred seventy-six Lebanese participants, comprising 151 controls 225 cases, were included. Clinical data obtained from questionnaires medical records. DNA isolated peripheral blood was genotyped for ACE1 rs1799752, ACE2 rs2074192, TMPRSS2 rs75603675 OAS1 rs107746771 using TaqMan assays, rs35074065 Sanger Sequencing. Candidate variants analyzed in with hospitalization univariate multivariate models. PRS constructed weighted sum of evaluated outcomes. In this study, there no statistically significant differences frequencies variant alleles within disease outcomes subgroups, after adjustment confounders. not associated susceptibility it however significantly predicted severity (P = 0.01). highlights importance testing key genes involved life cycle eventually measuring which proves be an important tool prognosis assessment vulnerable individuals, potentially enhancing patient care.

Language: Английский

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Association of ACE2 and TMPRSS2 towards COVID-19 susceptibility

Discover Life, Journal Year: 2024, Volume and Issue: 54(1)

Published: July 26, 2024

Abstract Severe Acute Respiratory Syndrome-Corona Virus-2 (SARS-CoV-2) is pneumonia like viral disease which was originated from Wuhan China in 2019. Besides its high morbidity and mortality, a lot of physiological, enzymatic, hormonal genetic imbalances had also been observed among Corona Virus Disease-19 (COVID-19) patients. The purpose the present study assessment comorbidities association single nucleotide polymorphisms (SNPs) Angiotensin-converting enzyme 2 ( ACE2 ) transmembrane protease serine TMPRSS gene COVID-19 A total 300 (healthy control n = 150 150) individuals were sampled genotyped for rs2285666 rs2070788 SNPs respectively. 92/150 (61.3%) male infected population, various age groups (age group 1: 1–15 yrs; 2: 16–30 3: 31–45; 4: 46 above) where most patients 4 (46 79/150 (52.7%) followed by 3 (31–45) 44/150 (29.3%). Logistic regression analysis showed that clinical features cough (90%) to be highest fever (80%), sore throat (76%) shortness breath (75%). Hypertension (51%), type II diabetes (48.4%), ischemic heart (43.3%) history found prevalent highly associated with individuals. For rs2285666, we risk both allele genotype while did not reveal association. It concluded current infects majority population. infection protective TMPRSS2 towards infection.

Language: Английский

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Role of ACE2 and TMPRSS2 polymorphisms on COVID-19 outcome and disease severity in adult patients: A prospective cohort study in a tertiary hospital, Indonesia

Narra J, Journal Year: 2024, Volume and Issue: 4(2), P. e919 - e919

Published: Aug. 12, 2024

Coronavirus disease 2019 (COVID-19) has led to a significant number of infections and deaths worldwide, yet its pathogenesis severity remain incompletely understood. Angiotensin-converting enzyme 2 (ACE2) transmembrane protease, serine (TMPRSS2), play crucial roles as receptors molecules responsible for the virus's entry into host cells, initiating infection process. Their polymorphisms have been extensively studied in relation COVID-19 severity. The aim this study was examine association

Language: Английский

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Evaluation of the association between clinical parameters and ADAM33 and ORMDL3 asthma gene single-nucleotide polymorphisms with the severity of COVID-19

International Immunopharmacology, Journal Year: 2023, Volume and Issue: 123, P. 110707 - 110707

Published: July 25, 2023

Language: Английский

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Genomic Insight into the COVID-19 Severity in MAFLD Patients: A Single-Center Prospective Cohort Study

Published: March 27, 2024

This study investigated the influence of single nucleotide polymorphisms (SNPs) in genes asso-ciated with interferon pathway (IFNAR2 rs2236757), antiviral response (OAS1 rs10774671, OAS3 rs10735079), and viral entry (ACE2 rs2074192) on COVID-19 severity their association non-alcoholic fatty liver disease (MAFLD). We did not observe a significant between SNPs severity. While IFNAR2 rs2236757 A allele correlated higher creatinine levels upon admission G lower band neu-trophils discharge, these findings require further investigation. The distribution OAS gene (rs10774671, rs10735079 differ MAFLD non-MAFLD patients. Our population's ACE2 rs2074192 genotypes alleles differed from European reference population. Overall, our suggest that specific may be major contributors to patient population, highlighting potential role other genetic factors environmental influences.

Language: Английский

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Gene variants rs5182, rs2074192, and rs4343 in the renin-angiotensin-aldosterone system are associated with symptom severity, higher odds of hospitalization, and death in COVID-19

International Journal of Infectious Diseases, Journal Year: 2024, Volume and Issue: 144, P. 107067 - 107067

Published: April 30, 2024

To analyze the gene variants of renin-angiotensin-aldosterone system and determine their association with severity outcome COVID-19.

Language: Английский

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SARS-CoV-2 Genomic Variants and Their Relationship with the Expressional and Genomic Profile of Angiotensin-Converting Enzyme 2 (ACE2) and Transmembrane Serine Protease 2 (TMPRSS2)

Microorganisms, Journal Year: 2024, Volume and Issue: 12(11), P. 2312 - 2312

Published: Nov. 14, 2024

Over the past four years, angiotensin-converting enzyme 2 (ACE2) and transmembrane serine protease (TMPRSS2) have been extensively studied, given their important role in SARS-CoV-2 replication; however, most studies failed to compare behavior face of different genomic variants. Therefore, this study evaluated influence variants ACE2/TMPRSS2 expressional profiles. To achieve this, 160 nasopharyngeal samples, previously detected with via RT-qPCR (June 2020-July 2022), were quantified for expression levels, also using RT-qPCR; variants, along polymorphisms coding genes, identified nanopore sequencing. In order appearance, B.1.1.28, Zeta, Gamma, Omicron study. The ACE2 levels higher when B.1.1.28 was present, possibly due ACE2/spike binding affinity; TMPRSS2 presence probably attributable inefficient usage pathway by other as well decrease transcription factors Omicron. rs2285666 (

Language: Английский

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Exploring non-coding genetic variability in ACE2: Functional annotation and in vitro validation of regulatory variants

Gene, Journal Year: 2024, Volume and Issue: 915, P. 148422 - 148422

Published: April 2, 2024

The surge in human whole-genome sequencing data has facilitated the study of non-coding region variations, yet understanding their biological significance remains a challenge. We used computational workflow to assess regulatory potential variants, with particular focus on Angiotensin Converting Enzyme 2 (ACE2) gene. This gene is crucial physiological processes and serves as entry point for severe acute respiratory syndrome coronavirus (SARS-CoV-2), virus causing disease 19 (COVID-19). In our analysis, using from gnomAD population database functional annotation, we identified 17 significant Single Nucleotide Variants (SNVs) ACE2, particularly its enhancers, promoters, 3' untranslated regions (UTRs). found preliminary evidence supporting impact some these variants ACE2 expression. Our detailed examination two SNVs, rs147718775 rs140394675, promoter revealed that co-occurring when mutated, significantly enhance activity, suggesting possible increase specific isoform method proves effective identifying interpreting impactful aiding further studies enhancing molecular bases monogenic complex traits.

Language: Английский

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