Development and validation of an automated and high-throughput quadruplex RT–ddPCR assay for the detection of influenza A, influenza B, respiratory syncytial virus, and SARS-CoV-2

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: March 28, 2025

The main detection method for viral respiratory infections is reverse transcription polymerase chain reaction (RT-PCR), but it susceptible to sample inhibitors and relies on a standard curve subjective thresholds quantify nucleic acid targets. However, droplet digital PCR (ddPCR), the third generation of with higher sensitivity accuracy, an effective tool absolute quantification viruses. In this study, we introduced AHQR-ddPCR, which automated high-throughput quadruplex ddPCR assay based QX ONE platform influenza A, B, syncytial virus, SARS-CoV-2 in single reaction. AHQR-ddPCR had analytical as low 0.65-0.78 copies/μL four viruses, exhibited excellent specificity, intraassay interassay precision, wide linear range each target. results clinical samples showed that good concordance better diagnostic compared RT-PCR. short, highly sensitive absolutely quantitative has performance, advantage detecting weakly positive samples, can effectively reduce false-negative powerful complement addition, great value virology research development molecular assays.

Language: Английский

Wastewater-based epidemiology of influenza viruses: a systematic review

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 986, P. 179706 - 179706

Published: May 30, 2025

Language: Английский

Evaluation of PCR-enhancing approaches to reduce inhibition in wastewater samples and enhance viral load measurements

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 955, P. 176768 - 176768

Published: Oct. 10, 2024

Language: Английский