Tracking epidemic viruses in wastewaters
Microbial Biotechnology,
Journal Year:
2024,
Volume and Issue:
17(10)
Published: Oct. 1, 2024
Classical
epidemiology
relies
on
incidence,
mortality
rates,
and
clinical
data
from
individual
testing,
which
can
be
challenging
for
many
countries.
Therefore,
innovative,
flexible,
cost-effective,
scalable
surveillance
techniques
are
needed.
Wastewater-based
(WBE)
has
emerged
as
a
highly
powerful
tool
in
this
regard.
WBE
analyses
substances
excreted
human
fluids
faeces
that
enter
the
sewer
system.
This
approach
provides
insights
into
community
health
status
lifestyle
habits.
serves
an
early
warning
system
viral
surveillance,
detecting
emergence
of
new
pathogens,
changes
incidence
identifying
future
trends,
studying
outbreaks,
informing
performance
action
plans.
While
long
been
used
to
study
different
viruses
such
poliovirus
norovirus,
its
implementation
surged
due
pandemic
caused
by
Severe
Acute
Respiratory
Syndrome
Coronavirus
2.
led
establishment
wastewater
programmes
at
international,
national,
levels,
remain
operational.
Furthermore,
is
increasingly
applied
other
including
antibiotic
resistance
bacteria,
parasites,
fungi,
emerging
viruses,
with
methodologies
being
developed.
Consequently,
primary
focus
now
creating
international
frameworks
enhance
states'
preparedness
against
risks.
However,
there
remains
considerable
work
done,
particularly
integrating
principles
One
Health
epidemiological
acknowledge
interconnectedness
humans,
animals,
environment
pathogen
transmission.
Thus,
broader
analysing
three
pillars
must
developed,
transitioning
environmental
establishing
routine
practice
public
health.
Language: Английский
Evaluating Interlaboratory Variability in Wastewater-Based COVID-19 Surveillance
Microorganisms,
Journal Year:
2025,
Volume and Issue:
13(3), P. 526 - 526
Published: Feb. 27, 2025
Wastewater-based
environmental
surveillance
enables
the
monitoring
of
SARS-CoV-2
dynamics
within
populations,
offering
critical
epidemiological
insights.
Numerous
workflows
for
tracking
have
been
developed
globally,
underscoring
need
interlaboratory
comparisons
to
ensure
data
consistency
and
comparability.
An
inter-calibration
test
was
conducted
among
laboratories
network
in
wastewater
samples
across
Lombardy
region
(Italy).
The
aimed
evaluate
reliability
identify
potential
sources
variability
using
robust
statistical
approaches.
Three
were
analyzed
parallel
by
four
identical
pre-analytical
(PEG-8000-based
centrifugation)
analytical
processes
(qPCR
targeting
N1/N3
Orf-1ab).
A
two-way
ANOVA
framework
Generalized
Linear
Models
applied,
multiple
pairwise
performed
Bonferroni
post
hoc
test.
analysis
revealed
that
primary
source
results
associated
with
phase.
This
likely
influenced
differences
standard
curves
used
quantify
concentrations,
as
well
size
treatment
plants.
findings
this
study
highlight
importance
testing
verifying
determinations
identifying
key
variation.
Language: Английский
Analysis Insights to Support the Use of Wastewater and Environmental Surveillance Data for Infectious Diseases and Pandemic Preparedness
Epidemics,
Journal Year:
2025,
Volume and Issue:
unknown, P. 100825 - 100825
Published: March 1, 2025
Wastewater-based
epidemiology
is
the
detection
of
pathogens
from
sewage
systems
and
interpretation
these
data
to
improve
public
health.
Its
use
has
increased
in
scope
since
2020,
when
it
was
demonstrated
that
SARS-CoV-2
RNA
could
be
successfully
extracted
wastewater
affected
populations.
In
this
Perspective
we
provide
an
overview
recent
advances
pathogen
within
wastewater,
propose
a
framework
for
identifying
utility
sampling
suggest
areas
where
analytics
require
development.
Ensuring
both
collection
analysis
are
tailored
towards
key
questions
at
different
stages
epidemic
will
inference
made.
For
analyses
useful
methods
determine
absence
infection,
early
reliably
estimate
trajectories
prevalence,
detect
novel
variants
without
reliance
on
consensus
sequences.
This
research
area
included
many
innovations
have
improved
collected
optimistic
innovation
continue
future.
Language: Английский