Circulating Cell-Free DNA as an Epigenetic Biomarker for Early Diabetic Retinopathy: A Narrative Review DOI Creative Commons
Boaz Li,

Megan M. Yim,

Yi Jin

et al.

Diagnostics, Journal Year: 2025, Volume and Issue: 15(9), P. 1161 - 1161

Published: May 2, 2025

Diabetic retinopathy (DR), a complication of type 2 diabetes mellitus (T2DM), is typically asymptomatic in its early stages. Diagnosis relies on routine fundoscopy for the clinical detection microvascular abnormalities. However, permanent retinal damage may occur well before signs are appreciable. In stages DR, retina undergoes distinct epigenetic changes, including DNA methylation and histone modifications. Recent evidence supports unique ‘signatures’ patients with DR compared to non-diabetic controls. These ‘signature’ sequences be specific circulate peripheral blood form cell-free (cfDNA). this review, we explore literature application cfDNA sampling as an early, non-invasive, accessible assessment tool detection. First, summarize known signatures DR. Next, review current sequencing technologies used detection, such magnetic bead-based enrichment, next-generation sequencing, bisulfite sequencing. Finally, outline research limitations emerging areas study which aim improve utility evaluation.

Language: Английский

Biomarkers in lung cancer treatment DOI

Rekha Thiruvengadam,

Carmelin Durai Singh,

Benod Kumar Kondapavuluri

et al.

Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 120267 - 120267

Published: March 1, 2025

Language: Английский

Citations

0
Circulating Cell-Free DNA as an Epigenetic Biomarker for Early Diabetic Retinopathy: A Narrative Review DOI Creative Commons
Boaz Li,

Megan M. Yim,

Yi Jin

et al.

Diagnostics, Journal Year: 2025, Volume and Issue: 15(9), P. 1161 - 1161

Published: May 2, 2025

Diabetic retinopathy (DR), a complication of type 2 diabetes mellitus (T2DM), is typically asymptomatic in its early stages. Diagnosis relies on routine fundoscopy for the clinical detection microvascular abnormalities. However, permanent retinal damage may occur well before signs are appreciable. In stages DR, retina undergoes distinct epigenetic changes, including DNA methylation and histone modifications. Recent evidence supports unique ‘signatures’ patients with DR compared to non-diabetic controls. These ‘signature’ sequences be specific circulate peripheral blood form cell-free (cfDNA). this review, we explore literature application cfDNA sampling as an early, non-invasive, accessible assessment tool detection. First, summarize known signatures DR. Next, review current sequencing technologies used detection, such magnetic bead-based enrichment, next-generation sequencing, bisulfite sequencing. Finally, outline research limitations emerging areas study which aim improve utility evaluation.

Language: Английский

Citations

0