Innovative strategy for extraction of green cardamom via Super critical fluid extractor at different levels of pressure with its application against microorganisms in vitro and in silico
Abstract
There
is
broad
approach
in
the
search
for
resource-use
strategies
that
are
both
economically
viable
and
value-based
extraction
of
active
compounds
from
various
plants.
Supercritical
fluid
(SFE),
has
become
a
popular
technique
significant
plant-based
compounds.
Our
investigation
contrasted
yield,
biological
functions
phytochemical
compositions
green
cardamom
extracts
generated
with
SFE
at
100,
200,
300
bar
pressures.
The
maximal
obtained
weight
was
0.279
gm
upon
applying
bar.
proportional
elevation
levels
most
phenolic
which
detected
using
HPLC
raising
pressure
extraction.
Antimicrobial
action
extract
types
towards
test
food-born
microbes
where
produced
had
antimicrobial
Bacillus
subtilis ,
Candida
albicans
inhibition
zones
23.33
±
0.58,
22.17
1.04
mm,
subsequently.
Antibiofilm
hemolysis
presence
forms
reveal
potential
impact
to
enhance
reach
level
Killing
kinetics
assay
showed
gradual
raise
versus
time
points
best
outcomes.
Transmission
electron
microscopy
visualized
alterations
ultrastructure
features
B.
subtilis
C.
molecular
docking
performance
main
constituents
gallic
acid
syringic
against
(PDB
ID:
5VX6)
S.
aureus
3V8J)
Molecular
Operating
Environment
(MOE)
software
evaluated.
scores
(S),
RMSD_refine
values,
energy
terms
(E_conf,
E_place,
E_score1,
E_refine,
E_score2)
were
analyzed
assess
binding
affinities.
Key
interactions,
including
hydrogen
bonds,
identified,
distances
energies
quantified.
Syringic
exhibited
better
(S
=
-4.27
-5.04)
compared
-4.11
-4.68)
across
targets.
Interactions
residues
like
GLU
187
ARG
172
5VX6,
ASP
239
3V8J,
highlighted
critical
motifs.
These
results
suggest
as
inhibitors
bacterial
targets,
warranting
further
experimental
validation.
Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown
Published: April 29, 2025
Language: Английский