Comparative evaluation of free radical scavenging activity and total metabolite profiles among 30 macrofungi species
Bioresources and Bioprocessing,
Journal Year:
2025,
Volume and Issue:
12(1)
Published: Feb. 21, 2025
Abstract
The
high
antioxidant
content
of
mushrooms
such
as
polyphenols,
polysaccharides,
carotenoids,
ergothioneine,
glutathione,
vitamins,
and
other
compounds,
has
sparked
interest
in
their
potential
use
preventive
therapeutic
medicine.
Therefore,
this
study
aimed
to
evaluate
the
various
macrofungi
by
measuring
1,1-diphenyl-2-picryl-hydrazyl
(DPPH)
radical
scavenging
activity
quantifying
two
classes
compounds:
phenolics
(TPC)
both
endo-
(IPS)
exopolysaccharides
(EPS).
Species-specific
abilities
produce
mycelium,
polyphenols
were
observed
under
submerged
cultivation
without
agitation
over
14
days.
Mycelium
productivity
(P
M
)
ranged
significantly
from
292
±
11
1110
37
mg·L⁻
1
·day⁻
,
with
highest
values
Ophiocordyceps
sinensis
(1110
),
Pleurotus
djamor
(1090
Cordyceps
militaris
(1080
90
mg·
L⁻
).
DPPH
inhibition
extracts
varied
4.30
0.20
87.9
0.80%,
while
TPC
0.35
0.10
34.6
0.80
mg
GAE/g
d.w.
Lentinula
edodes
was
best
source
produced
IPS
(10.3
g·L
−1
Hypsizygus
marmoreus
exhibited
EPS
(2.24
0.30
Overall,
levels
across
species
higher,
ranging
1.56
10.3
.
In
half
fungal
species,
mycelium
superior
compared
culture
broth.
However,
majority
(66.7%),
higher
broth
than
mycelium.
Distinct
metabolite
profiles
among
different
isolates.
Notably,
Fomitopsis
pinicola
L.
showed
TPC.
depending
on
medium,
solvents
used.
(90%)
(38.5
0.40
d.w.)
methanol
(MeOH)
extract
F.
grown
Sabouraud
dextrose
medium.
Similarly,
MeOH
ethyl
acetate
(EtOAc)
demonstrated
strong
(over
87%),
maximum
(40.0
0.60
detected
water
(H
2
O)
cultivated
glucose-peptone-yeast
A
positive
correlation
found
between
activity,
Pearson’s
coefficient
0.6615
for
0.1192
broth,
0.8924
0.7143
respectively.
Both
exhibit
significant
natural
antioxidants.
These
can
be
applied
nutraceuticals,
food
products,
nutritional
supplements,
offering
diverse
health
benefits.
Additionally,
is
a
pioneering
exploration
Auriporia
aurea
Hohenbuehelia
myxotricha
Lepista
luscina
Oxyporus
obducens
Pseudospongipellis
litschaueri
highlighting
largely
unexplored
applications.
Graphical
Language: Английский
Effect of the composition of a biologically active dietary supplement with macrofungi mycelia on its antioxidant activity
Regulatory Mechanisms in Biosystems,
Journal Year:
2024,
Volume and Issue:
15(4), P. 932 - 938
Published: Nov. 6, 2024
The
global
demand
for
macrofungi
is
driven
by
their
rich
content
of
biologically
active
substances
and
trends
such
as
the
need
natural
medicines,
growing
popularity
vegan
diets,
increased
consumer
interest
in
therapeutic
prophylactic
dietary
supplements,
expanding
use
fungi
food,
pharmaceutical,
cosmetic
industries.
aim
this
study
was
to
investigate
effect
composition
a
supplement
based
on
mixture
macrofungal
mycelium
total
phenolic
antioxidant
activity
supplement.
Fomitopsis
pinicola,
Pleurotus
ostreatus
Trametes
versicolor
were
obtained
through
controlled
submerged
static
cultivation
suitable
media.
powdered
mycelia
F.
P.
ostreatus,
T.
versicolor,
which
are
cubic
plate-shaped
conglomerates
with
rounded
edges,
translucent,
smooth
surface,
mixed
excipients
granulated.
Pharmacotechnological
characteristics
compressibility,
powder
flowability,
moisture
content,
well
dosage
uniformity
disintegration
time
hard
capsules
significantly
varied
depending
granulate
composition.
each
mixture,
selected
granulates,
determined.
Differences
insignificant.
Determination
these
parameters
pharmacotechnological
granulates
allowed
identification
most
excipients:
lactose
monohydrate
(5%),
mannitol:microcrystalline
cellulose
101
ratio
2:1
(10%),
sodium
croscarmellose
(1%),
0.7%
solution
carboxymethyl
(4%).
This
contains
29.83
±
0.49
mg
GAE/g
can
neutralize
DPPH
free
radical
scavenging
86.53
0.62%.
results
confirm
prospects
using
promising
raw
material
development
new
activity.
Language: Английский