Growth Factor Release within Liquid and Solid PRF

Journal of Clinical Medicine, Journal Year: 2022, Volume and Issue: 11(17), P. 5070 - 5070

Published: Aug. 29, 2022

Aim: The purpose of this study was to obtain data concerning growth factor release within liquid and solid platelet-rich fibrin (PRF) matrices estimate the amount potential interindividual variations as a basis for further preclinical clinical trials. Therefore, we aimed determine possible differences in factors between PRF. Materials Methods: Blood samples obtained from four subjects were processed both PRF using standard centrifugation protocol. Five (vascular endothelial factor, VEGF; epidermal EGF; platelet-derived factor-BB, PDGF-BB; transforming factor-β1, TGF-β1; matrix metallopeptidase 9, MMP-9) have been evaluated at six time points by ELISA over total observation period 10 days (1 h, 7 1 d, 2 d). Results: Growth could be measured all each point. Comparing matrices, no significant detected (p > 0.05). mean VEGF, TGFβ-1, PDGF-BB, MMP-9 raised peak point five (day 7) matrices. VEGF lower than PRF, whereas those PDGF-BB higher points. EGF had its already two after h (hour solid: = 180 pg/mL, SD 81; liquid: 218 64), declined rapidly until day 2, second slight on groups 182 189; 81 70). Conclusions: This with little variations. Further trials are needed precisely analyze larger better understand their effects wound healing different indications.

Language: Английский

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Antimicrobial effect of platelet‐rich fibrin: A systematic review of in vitro evidence‐based studies

Periodontology 2000, Journal Year: 2023, Volume and Issue: 94(1), P. 131 - 142

Published: Sept. 22, 2023

Abstract This systematic review (SR) aimed to evaluate the antimicrobial potential of different types platelet‐rich fibrin (PRF) often used in regenerative treatments. An electronic search was performed four databases and Gray literature for articles published until January, 2023. The eligibility criteria comprised vitro studies that evaluated effect PRF. For analysis risk bias within studies, modified OHAT (Office Health Assessment Translation) tool used. evaluation results, a qualitative critical carried out synthesis results primary studies. Sixteen between 2013 2021 were included this SR. effects PRF variations (PRF, injectable [I‐PRF], with silver nanoparticles [agNP‐PRF], horizontal [H‐PRF]), analyzed against 16 bacteria from oral, periodontal, endodontic environments. All showed significant action, antibacterial efficacy being more expressive than fungal one. I‐PRF, H‐PRF, agNP‐PRF subtypes improve activity. According analysis, no study classified as having high bias. Evidence suggests have activity, bacterial action greater fungal. Evolutions such Future analyzing clinical these platelets are fundamental. SR registered INPLASY under number INPLASY202340016.

Language: Английский

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Extraction Socket Augmentation with Autologous Platelet-Rich Fibrin (PRF): The Rationale for Socket Augmentation

Dentistry Journal, Journal Year: 2023, Volume and Issue: 11(8), P. 196 - 196

Published: Aug. 14, 2023

After tooth extraction, the alveolar ridge undergoes a physiological process of remodelling and disuse atrophy. Socket augmentation (SA) has been shown to preserve bone volume in order facilitate implant placement reduce need for staged grafting at later date. Although autogenic be gold standard regeneration, it significant disadvantages. To prevent post-extraction volumetric alterations resorption occurring, alternative materials, including xenografts, alloplasts, allografts, have used successfully fresh extraction sites. However, these materials act mostly as bio-scaffolds require slower integration period 6–8 months prior placement. Recently, use autologous platelet-rich fibrin (PRF) advocated alongside socket method bio-enhancement healing soft hard tissues. PRF contains platelet-derived growth factors, hormones, bioactive components such cytokines that promote angiogenesis tissue regeneration during wound healing. The aim this article is review evidence base SA technique Clinical benefits will discussed with reference two cases. Therefore, narrative discuss changes, importance SA, role management site defects when immediate not possible or contraindicated.

Language: Английский

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Platelet rich fibrin as a bioactive matrix with proosteogenic and proangiogenic properties on human healthy primary cells in vitro

Platelets, Journal Year: 2024, Volume and Issue: 35(1)

Published: Feb. 23, 2024

Blood concentrates like platelet rich fibrin (PRF) have been established as a potential autologous source of cells and growth factors with regenerative properties in the field dentistry medicine. To further analyze effect PRF on bone tissue regeneration, this study investigated influence liquid matrices human healthy primary osteoblasts (pOB) co-cultures composed pOB dermal vascular endothelial (HDMEC) vitro model for regeneration. Special attention was paid to mediated osteoblastic differentiation angiogenesis. Based low-speed centrifugation concept, were treated indirectly prepared low (44 g) high relative centrifugal force (710 before osteoblast proliferation assessed via gene protein expression analyses immunofluorescence. The results revealed PRF-mediated positive osteogenic accompanied by increased concentration upregulated factors. Furthermore, it could be shown that treatment resulted an formation angiogenic structures mimic co-culture induced release proangiogenic effects proliferation, vascularization more evident when RCF applied cells. In conclusion, possess proosteogenic, potentially osteoconductive well properties, making beneficial tool

Language: Английский

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Using platelet concentrates to treat maxillofacial tissue lesions

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 7, 2025

Platelet concentrates (PCs), which are blood products that abundant in platelets and growth factors, have become pivotal treating maxillofacial tissue lesions due to their capacity for promoting bone soft recovery. This review will provide some recent progress of the use platelet treat on tissues. We reviewed mechanisms by PCs promote wound healing recovery summarized application treatment tissues, including medication-related osteonecrosis jaw, post-extraction healing, implant surgery, temporomandibular joint diseases, periodontal restoration. PC promotes attachment proliferation osteoblasts, as well synthesis deposition collagen fibers stimulating AFK pathway releasing factors cytokines, such secreting GFs, VEGF, TGF-β, etc. They also induce angiogenesis, inhibit resorption, relieve symptoms, reduce postoperative complications maintain stability. may be used an adjuvant therapy However, more studies should refine preparation methods establish a foundation extensive application.

Language: Английский

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One-Stage Surgical Management of an Asymptomatic Maxillary Sinus Mucocele with Immediate Lateral Sinus Lift and Simultaneous Implant Placement: A Case Report

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(6), P. 1946 - 1946

Published: March 13, 2025

Background: The relationship between dental implants and sinus mucoceles is an area of growing interest in oral maxillofacial surgery, as therapeutic approaches for these conditions remain controversial. This case report presents a 48-year-old male with no significant medical history who sought care due to recurrent abscesses on the distal abutment five-unit maxillary bridge. Clinical radiographic evaluations, including Cone-Beam Computed Tomography (CBCT), revealed pathologic lesion associated second molar, insufficient alveolar bone height posterior maxilla, radiopaque mass floor. Methods: A one-stage surgical approach was planned, involving molar extraction, cyst removal, floor elevation, simultaneous implant placement. prosthetic restoration completed six months postoperatively. Results: Over 88-month follow-up period, complications were observed, patient reported high satisfaction restoration’s function aesthetics. Conclusions: highlights single-stage strategy’s feasibility long-term success managing asymptomatic while optimizing placement rehabilitation.

Language: Английский

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Development of Semi-Automated Image-Based Analysis Tool for CBCT Evaluation of Alveolar Ridge Changes After Tooth Extraction

Bioengineering, Journal Year: 2025, Volume and Issue: 12(3), P. 307 - 307

Published: March 18, 2025

Following tooth extraction, the bone structure is prone to atrophic changes. Alveolar ridge resorption can compromise subsequent implant treatment not only at extraction site itself but also by affecting support of adjacent teeth. Various techniques, including use graft materials or autologous blood concentrates for socket preservation, aim counteract this process. The efficacy such methods be evaluated non-invasively through radiological analysis treated region. However, existing evaluation often focus on isolated areas socket, limiting their accuracy in assessing overall regeneration. This study introduces a novel, non-invasive, and semi-automated image-based method that enables more comprehensive preservation using CBCT data. Developed with open-source software “Fiji” (v2.15.0; based ImageJ), approach assesses changes multiple horizontal vertical positions, creating near three-dimensional representation resorptive By analyzing entire region around rather than selected regions, provides precise reproducible assessment alveolar preservation. Although requires some processing time focuses exclusively evaluation, it offers greater conventional methods. Its standardized objective nature makes valuable tool clinical research, facilitating reliable comparisons different strategies.

Language: Английский

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Platelet-Rich Fibrin in MRONJ Management: A Prospective Comparative Study on Its Effectiveness in Prevention and Treatment

Medicina, Journal Year: 2025, Volume and Issue: 61(4), P. 625 - 625

Published: March 28, 2025

Background and Objectives: Medication-related osteonecrosis of the jaw (MRONJ) was first recognized as a disease entity reported in literature 2003. Within few years, incidence MRONJ has increased significantly, to point where now it can be seen every dental clinic around world. Its prevention management still remain major challenges for dentists oral maxillofacial surgeons. Materials Methods: This prospective clinical study conducted at Oral Maxillofacial Surgery Clinic Timisoara 6-month period included total 85 patients under chronic antiresorptive antiangiogenic medication. There were two groups patients: G1 received PRF growth factors, while other group, G2, treated with classical surgical methods. Post-operative wound healing assessed 2, 4, 8 weeks by monitoring absence local gingival dehiscence, suprainfection, or loco-regional fistulas, both cases extractions sequestrectomies cases. Results: The use post-extraction sockets predisposed developing aids 96% cases, compared not used, which normal occurred only 64.29% patients; there significant difference between (p = 0.016). In confirmed application after excisional debridement necrotic bone does appear have same therapeutic value post-extractional sockets, p-value 0.299 indicating no statistical significance. Conclusions: considered an effective approach preventing osteonecrotic complications following treatment. Additional studies are needed establish its role

Language: Английский

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Platelet rich fibrin and commercial sealants for dural closure in neurosurgery: An in vitro study

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(4), P. e0319349 - e0319349

Published: April 1, 2025

Background Watertight closure of the dura mater is essential after neurosurgical interventions to avoid complications such meningitis, intracranial hypotension and surgical site infections. In addition conventional suturing techniques, various biomaterials have been proposed as sealants minimize risk leakage. We previously reported that patient derived platelet-rich fibrin (PRF), an autologous biomaterial, significantly improves watertight dural when applied onlay over a standard running suture. To demonstrate efficacy this method, we aimed compare orthobiological approach with other commercially available sealants. Methods utilized in vitro testing device allowed for assessment via hydrostatic testing. On our phantom using freshly harvested bovine maters, standardized 20-mm incision was closed suture, leak pressure measured initially without (primary pressure) subsequently on lay augmentation (secondary either PRF, fibrinogen- thrombin-coated collagen patch (TachoSil®), matrix graft (DuraGen®), Polyethylenglykol (PEG) polyethylene glycol ester trilysine amine hydrogel solution (DuraSeal®), glycol, protein-reactive monomer coated (Hemopatch®) or polyethylenimine component autospray sealant (Adherus®). Results The outcomes usage suture exhibited substantial improvement compared alone. Specifically, median improved by 3.5 times, where combined able withstand 38 cm H 2 O, whereas alone had 11 O. Upon evaluating performance sealants, identified two categories sealants: medium group (30 ± O) comprised Adherus® (31 O), DuraGen® Hemopatch® (28 high-performance composed DuraSeal® (45.5 TachoSil® (41 O). Patient-derived PRF max 45 falling into group. Of all tested study, based demonstrated lowest variance among Conclusions Autologous two-step procedure displayed enhanced reinforced mater, exhibiting four-fold increase Among commonly commercial it ranked second consistency its performance.

Language: Английский

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PRF and PRP in Dentistry: An Umbrella Review

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(9), P. 3224 - 3224

Published: May 6, 2025

Introduction: Platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) utilize autologous blood share the objective of leveraging blood-derived growth factors to enhance body’s natural healing process. A large extensive use has been made in various branches dentistry. Methods: total 4175 records were identified from electronic search, specifically 291 BioMed Central, 3406 MEDLINE/PubMed, 304 Cochrane library databases, 174 PROSPERO register. This review was performed relation PRISMA flow chart annotated Results: In total, 3416 title abstracts screened, a 40 systematic reviews finally included present umbrella review. Conclusions: Research supports PRF PRP different fields is huge potential for patient but also doctor as these products are have zero cost. However, further studies needed, especially RCTs, clearer evidence on role PRP.

Language: Английский

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