ACS Synthetic Biology,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 17, 2024
The
efficiency
of
valuable
metabolite
production
by
engineered
microorganisms
underscores
the
importance
stable
and
controllable
gene
expression.
While
plasmid-based
methods
offer
flexibility,
integrating
genes
into
host
chromosomes
can
establish
stability
without
selection
pressure.
However,
achieving
site-directed
multicopy
integration
presents
challenges,
including
site
stability.
We
introduced
a
method
using
novel
dual-plasmid
mini-Tn5
system
to
insert
Journal of Clinical Cardiology,
Journal Year:
2024,
Volume and Issue:
5(1), P. 6 - 29
Published: Jan. 5, 2024
Cardiovascular
diseases
(CVDs)
remain
a
leading
cause
of
morbidity
and
mortality
worldwide.
Effective
prevention
management
strategies
are
essential
to
reduce
the
burden
CVDs.
This
review
summarizes
recent
advances
in
In
terms
prevention,
lifestyle
modifications,
such
as
diet,
exercise,
smoking
cessation,
important
strategies.
Pharmacological
interventions,
including
statins,
antiplatelet
agents,
antihypertensive
medications,
have
also
shown
benefits.
Innovative
approaches,
genetic
testing
risk
stratification,
targeted
strategies,
use
mobile
health
technology,
being
explored.
management,
acute
reperfusion
therapy
for
myocardial
infarction,
heart
failure
stroke
improved
outcomes.
Chronic
medical
failure,
arrhythmia
device
precision
medicine
personalized
treatment,
artificial
intelligence
diagnosis
traditional
Chinese
regenerative
medicine,
investigated.
While
CVDs
promising,
challenges
implementing
these
cost-effectiveness,
access
care,
resistance
change
clinical
practice.
Continued
research
innovation
improve
patient
Frontiers in Chemistry,
Journal Year:
2023,
Volume and Issue:
11
Published: Sept. 28, 2023
The
use
of
biomaterials
in
delivering
CRISPR/Cas9
for
gene
therapy
infectious
diseases
holds
tremendous
potential.
This
innovative
approach
combines
the
advantages
with
protective
properties
biomaterials,
enabling
accurate
and
efficient
editing
while
enhancing
safety.
Biomaterials
play
a
vital
role
shielding
components,
such
as
lipid
nanoparticles
or
viral
vectors,
from
immunological
processes
degradation,
extending
their
effectiveness.
By
utilizing
flexibility
tailored
systems
can
be
designed
to
address
specific
genetic
diseases,
paving
way
personalized
therapeutics.
Furthermore,
this
delivery
method
offers
promising
avenues
combating
illnesses
by
precisely
modifying
pathogen
genomes,
reducing
pathogenicity.
facilitate
site-specific
modifications,
ensuring
effective
infected
cells
minimizing
off-target
effects.
However,
challenges
remain,
including
optimizing
efficiency,
effects,
long-term
safety,
establishing
scalable
production
techniques.
Thorough
research,
pre-clinical
investigations,
rigorous
safety
evaluations
are
imperative
successful
translation
laboratory
clinical
applications.
In
review,
we
discussed
how
using
revolutionizes
disease
treatment,
offering
precise
safe
capabilities
potential
significantly
improve
human
health
quality
life.
Biotechnology Journal,
Journal Year:
2024,
Volume and Issue:
19(2)
Published: Feb. 1, 2024
World's
population
is
elevating
at
an
alarming
rate
thus,
the
rising
demands
of
producing
crops
with
better
adaptability
to
biotic
and
abiotic
stresses,
superior
nutritional
as
well
morphological
qualities,
generation
high-yielding
varieties
have
led
encourage
development
new
plant
breeding
technologies.
The
availability
easy
accessibility
genome
sequences
for
a
number
crop
plants
various
editing
technologies
such
zinc
finger
nucleases
(ZFNs),
transcription
activator-like
effector
(TALENs)
has
opened
up
possibilities
develop
desirable
traits.
However,
these
approaches
limitation
being
more
expensive
having
complex
steps
time-consuming.
CRISPR/Cas
system
been
intensively
studied
allowing
versatile
target-specific
modifications
that
fruitfully
aid
in
novel
varieties.
It
advanced
promising
technology
potential
meet
hunger
needs
contribute
food
production
ever-growing
human
population.
This
review
summarizes
usage
tool
targeted
improvement
stress
resistance,
yield,
quality
traits
desired
plants.
Cells,
Journal Year:
2024,
Volume and Issue:
13(17), P. 1422 - 1422
Published: Aug. 26, 2024
Mechanical
force
is
the
basis
of
cardiovascular
development,
homeostasis,
and
diseases.
The
perception
response
mechanical
by
system
are
crucial.
However,
molecular
mechanisms
mediating
mechanotransduction
in
not
yet
understood.
PIEZO1,
a
novel
transmembrane
mechanosensitive
cation
channel
known
for
its
regulation
touch
sensation,
has
been
found
to
be
widely
expressed
mammalian
system.
In
this
review,
we
elucidate
role
mechanism
PIEZO1
as
sensor
disease
processes,
including
embryo
survival,
angiogenesis,
cardiac
development
repair,
vascular
inflammation,
lymphangiogenesis,
blood
pressure
regulation,
hypertrophy,
fibrosis,
ventricular
remodeling,
heart
failure.
We
further
summarize
chemical
molecules
targeting
potential
translational
applications.
Finally,
address
controversies
surrounding
emergent
concepts
challenges
future
Frontiers in Immunology,
Journal Year:
2025,
Volume and Issue:
16
Published: Feb. 26, 2025
Hypertension,
a
globally
prevalent
condition,
is
closely
associated
with
T
cell-mediated
inflammatory
responses.
Studies
have
shown
that
cells,
by
secreting
pro-inflammatory
cytokines
such
as
interferon-gamma
(IFN-γ),
Interleukin-17
(IL-17),
and
Tumor
necrosis
factor-alpha
(TNF-α),
directly
lead
to
vascular
dysfunction
elevated
blood
pressure.
The
activation
of
Th1
Th17
cell
subsets,
along
the
regulatory
cells
(Tregs),
critical
mechanism
in
onset
progression
hypertension.
This
review
explores
role
pathophysiology
hypertension
discusses
potential
therapeutic
strategies
targeting
regulation,
immunotherapy
gene-editing
technologies.
These
emerging
treatments
hold
promise
for
providing
personalized
options
hypertensive
patients,
reducing
complications,
improving
treatment
outcomes.
