Wound Closure Promotion by Leucine-Based Pseudo-Proteins: An In Vitro Study DOI Open Access

Mariam Ksovreli,

Tinatin Kachlishvili,

Mariam Skhvitaridze

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9641 - 9641

Published: Sept. 6, 2024

Our research explores leucine-based pseudo-proteins (LPPs) for advanced wound dressings, focusing on their effects healing in an vitro model. We assessed three types of LPP films ability to enhance closure rates and modulate cytokine production. They all significantly improved compared traditional methods, with the 8L6 copolymer showing most pronounced effects. Notably, latter exhibited optimal profile: initial burst pro-inflammatory TNF-α, followed by a controlled release IL-6 during proliferative phase significant increase anti-inflammatory IL-10 remodeling. This balanced response suggests that film not only accelerates but also supports well-regulated process, potentially reducing fibrosis abnormal scarring, underscoring potential LPPs as dressing materials. Future will aim elucidate specific signaling pathways activated better understand its mechanism action. Overall, offer promising approach improving care could lead more effective treatments complex wounds.

Language: Английский

Emerging Delivery Systems for Enabling Precision Nucleic Acid Therapeutics DOI

Xiaochun Bian,

Liping Zhou,

Zhiwei Luo

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Nucleic acid therapeutics represent a highly promising treatment approach in modern medicine, treating diseases at the genetic level. However, these face numerous challenges practical applications, particularly regarding their stability, effectiveness, cellular uptake efficiency, and limitations delivering them specifically to target tissues. To overcome obstacles, researchers have developed various innovative delivery systems, including viral vectors, lipid nanoparticles, polymer inorganic protein carriers, exosomes, antibody oligonucleotide conjugates, DNA nanostructure-based systems. These systems enhance therapeutic efficacy of nucleic drugs by improving targeting specificity, half-life vivo. In this review, we systematically discuss different types drugs, analyze major barriers encountered delivery, summarize current research progress emerging We also highlight latest advancements application for diseases, infectious cancer, brain wound healing. This review aims provide comprehensive overview drug systems' status future directions integrating nanotechnology, biomaterials science, gene editing technologies, emphasizing transformative potential precision medicine.

Language: Английский

Citations

2
Recent advances in hyaluronic acid-based hydrogels for diabetic wound healing DOI
Chenguang Liu,

Ronger Ai,

Bi-zhi Liu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 304, P. 140797 - 140797

Published: Feb. 7, 2025

Language: Английский

Citations

1
Strategies for promoting neurovascularization in bone regeneration DOI Creative Commons
Xinling Li, Yuqing Zhao, Miao Li

et al.

Military Medical Research, Journal Year: 2025, Volume and Issue: 12(1)

Published: March 3, 2025

Abstract Bone tissue relies on the intricate interplay between blood vessels and nerve fibers, both are essential for many physiological pathological processes of skeletal system. Blood provide necessary oxygen nutrients to bone tissues, remove metabolic waste. Concomitantly, fibers precede during growth, promote vascularization, influence cells by secreting neurotransmitters stimulate osteogenesis. Despite critical roles components, current biomaterials generally focus enhancing intraosseous vessel repair, while often neglecting contribution nerves. Understanding distribution main functions in is crucial developing effective engineering. This review first explores anatomy highlighting their vital embryonic development, metabolism, repair. It covers innovative regeneration strategies directed at accelerating intrabony neurovascular system over past 10 years. The issues covered included material properties (stiffness, surface topography, pore structures, conductivity, piezoelectricity) acellular biological factors [neurotrophins, peptides, ribonucleic acids (RNAs), inorganic ions, exosomes]. Major challenges encountered neurovascularized materials clinical translation have also been highlighted. Furthermore, discusses future research directions potential developments aimed producing repair that more accurately mimic natural healing tissue. will serve as a valuable reference researchers clinicians novel into practice. By bridging gap experimental practical application, these advancements transform treatment defects significantly improve quality life patients with bone-related conditions.

Language: Английский

Citations

1
Rhein-loaded chitosan nanoparticles for treatment of MRSA-infected wound DOI

Shuang Cai,

Linlin Wang,

Xilong Cui

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 279, P. 135360 - 135360

Published: Sept. 5, 2024

Language: Английский

Citations

4
Mechanism underlying the involvement of CXCR4/CXCL12 in diabetic wound healing and prospects for responsive hydrogel-loaded CXCR4 formulations DOI Creative Commons
Lingli Wang,

Fengsong Nie,

Zhaoyu Lu

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 16, 2025

Diabetes mellitus is a prevalent chronic disease, often leading to complications, with wounds being among the most challenging. Impairment of CXCR4/CXCL12 signaling pathway, which plays key role in cell mobilization, migration, and angiogenesis, significantly hampers wound healing process diabetic patients. Modulation this pathway using CXCR4-targeted agents has shown promise restoring repair capabilities. Additionally, development responsive hydrogels capable adapting external stimuli offers powerful platform for drug delivery management. These hydrogels, when loaded CXCR4 agonists or antagonists, enable controlled release real-time therapeutic modulation. Integrating such existing strategies may provide an innovative effective solution overcoming challenges associated treatment.

Language: Английский

Citations

0
A Biocompatible β-Cyclodextrin Inclusion Complex Containing Natural Extracts: A Promising Antibiofilm Agent DOI Creative Commons
Obaydah Abd Alkader Alabrahim, Mostafa Fytory,

Ahmed M. Abou‐Shanab

et al.

Nanoscale Advances, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Biofilms still pose a significant healthcare challenge, worldwide. βCD nanoparticles loaded with Boswellia sacra essential oils (BOS) hold promise for effectively preventing and disrupting bacterial biofilms.

Language: Английский

Citations

0
Immunomodulatory hydrogels for tissue repair and regeneration DOI Creative Commons
Xiangjun Liu,

Yue Hu,

Yikun Ju

et al.

APL Materials, Journal Year: 2024, Volume and Issue: 12(8)

Published: Aug. 1, 2024

Tissue repair and regeneration have long been a focus of research, with growing evidence highlighting the role played by immunomodulation. The tissue cells can be significantly promoted through localized immunomodulation tissues. Hydrogels are increasingly used in this field due to their biocompatibility, versatility, relative ease fabrication. This Review highlights critical inflammation immune development complex process after injury, dynamic interactions between that ultimately influence outcome healing. We also summarize precise functions responses various injury scenarios, which for harnessing full potential immunomodulatory strategies promote repair. discuss recent advances drug-carrying hydrogels, offer promising avenues control environment constructive Further our understanding mechanisms driving cell behavior during expected foster effective hydrogel-based regeneration.

Language: Английский

Citations

2
Analysis of risk factors related to chronic non‐healing wound infection and the construction of a clinical prediction model DOI
Jing Liu, Qiang He,

G. Bih-Fang Guo

et al.

Experimental Dermatology, Journal Year: 2024, Volume and Issue: 33(7)

Published: July 1, 2024

Abstract This study is aimed to analyse the risk factors associated with chronic non‐healing wound infections, establish a clinical prediction model, and validate its performance. Clinical data were retrospectively collected from 260 patients wounds treated in plastic surgery ward of Shanxi Provincial People's Hospital between January 2022 December 2023 who met inclusion criteria. Risk analysed, model was constructed using both single multifactor logistic regression analyses determine infections. The model's discrimination calibration assessed via concordance index (C‐index), receiver operating characteristic (ROC) curve curve. Multivariate analysis identified several independent for infection: long‐term smoking (odds ratio [OR]: 4.122, 95% CI: 3.412–5.312, p < 0.05), history diabetes (OR: 3.213, 2.867–4.521, elevated C‐reactive protein 2.981, 2.312–3.579, procalcitonin 2.253, 1.893–3.412, 0.05) reduced albumin 1.892, 1.322–3.112, 0.05). C‐index 0.762, corrected internal validation bootstrap method being 0.747. ROC indicated an area under (AUC) 0.762 (95% 0.702–0.822). Both AUC C‐indexes ranged 0.7 0.9, suggesting moderate‐to‐good predictive accuracy. chart demonstrated good fit ideal Long‐term smoking, diabetes, protein, are confirmed as bacterial infection wounds. based on these shows robust performance substantial value.

Language: Английский

Citations

0
Anti-mCD20 in combination with α-mCXCL13 monoclonal antibody inhibits anti-FVIII antibody development in hemophilia A mice DOI

Qiaoyun Zheng,

Kehan Lin,

Na Zhang

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 139, P. 112735 - 112735

Published: July 26, 2024

Language: Английский

Citations

0
Wound Closure Promotion by Leucine-Based Pseudo-Proteins: An In Vitro Study DOI Open Access

Mariam Ksovreli,

Tinatin Kachlishvili,

Mariam Skhvitaridze

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9641 - 9641

Published: Sept. 6, 2024

Our research explores leucine-based pseudo-proteins (LPPs) for advanced wound dressings, focusing on their effects healing in an vitro model. We assessed three types of LPP films ability to enhance closure rates and modulate cytokine production. They all significantly improved compared traditional methods, with the 8L6 copolymer showing most pronounced effects. Notably, latter exhibited optimal profile: initial burst pro-inflammatory TNF-α, followed by a controlled release IL-6 during proliferative phase significant increase anti-inflammatory IL-10 remodeling. This balanced response suggests that film not only accelerates but also supports well-regulated process, potentially reducing fibrosis abnormal scarring, underscoring potential LPPs as dressing materials. Future will aim elucidate specific signaling pathways activated better understand its mechanism action. Overall, offer promising approach improving care could lead more effective treatments complex wounds.

Language: Английский

Citations

0