Confocal Microscopy: Revolutionizing Three-Dimensional Imaging In Biological Research. DOI Open Access
Ashish Dwivedi

African Journal of Biomedical Research, Journal Year: 2024, Volume and Issue: unknown, P. 7662 - 7671

Published: Dec. 16, 2024

Confocal microscopy is a high-resolution imaging technique that uses laser scanning to create sharp, three-dimensional images of biological samples, improving the visualization complex specimens.Confocal has wide range applications including stem cell and biofilm analysis, disease diagnosis etc. owing its ability capture detailed cellular structures, allowing for detection abnormalities in tissue morphology organization.The growing interest this technology underscores importance understanding foundations effectively address challenges further advance applications.This review explores history, principles, confocal biology, highlighting trends, challenges, future advancements technology.

Language: Английский

A Mini Review of Quantitative Optical Technologies for Imaging Cell and Tissue Metabolism DOI Creative Commons
Audrey P. Fan,

Erick Alvarado,

Anton Block

et al.

Current Opinion in Biomedical Engineering, Journal Year: 2025, Volume and Issue: unknown, P. 100581 - 100581

Published: Feb. 1, 2025

Language: Английский

Citations

0
AI-based autofocusing of red blood cells in digital in-line holographic microscopy DOI
Jihwan Kim, Sang Joon Lee

Optics and Lasers in Engineering, Journal Year: 2025, Volume and Issue: 188, P. 108892 - 108892

Published: Feb. 22, 2025

Language: Английский

Citations

0
Morphological Parameters of 29994 sperm in a fertile male population—based on Papanicolaou staining and SSA-II Plus DOI Creative Commons
Longlong Fu, Fang Zhou, Guoping Chen

et al.

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16

Published: March 7, 2025

This study aims to provide reference data for sperm morphology in a healthy, fertile male population providing foundation future studies on infertility assessment and selection assisted reproductive technologies. The included 21 healthy participants, all of whom had partners who conceived within the past 12 months. Sperm samples were collected according WHO guidelines stained using Papanicolaou method. parameters, including head length, width, area, perimeter, ellipticity, acrosome measured Suiplus SSA-II Computer-Assisted Analysis (CASA) system. Statistical comparisons made between CASA traditional manual methods. percentage with normal was 9.98%. Detailed measurements, provided as values population. system demonstrated ability reduce subjective errors showed no significant differences count motility compared provides precise that enhance diagnostics treatment, particularly technologies like ICSI.

Language: Английский

Citations

0
Confocal Laser Scanning Platform Combined With In Situ High‐Resolution Quantitative Phase Imaging DOI
Wenjing Feng, Hongfei Suo, Ying Ma

et al.

Journal of Biophotonics, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

This paper introduces an advanced dual-modality imaging platform by integrating a confocal laser scanning/imaging module with flat-fielding quantitative phase contrast microscopy (FF-QPCM). The enables phase/fluorescence of the same samples high resolution and contrast. Using this platform, we observed that fluorescence excitation causes minimal damage to cells, whereas wide-field (WFE) results in significant damage, particularly mitochondrial fragmentation notable alterations values. Additionally, situ was conducted on live COS7 cells specific region irradiated laser. demonstrated 5-min continuous irradiation affects only fluorescently labeled mitochondria, causing dysfunction throughout cell. These findings suggest FF-QPCM offers highly efficient, sensitive, information-rich tool for cell biology research, broad application potential.

Language: Английский

Citations

0
Holographic tomography of the diatom Skeletonema pseudocostatum used as a bioindicator of heavy metal-polluted waters DOI Creative Commons
Maria Antonietta Ferrara,

Elena Cavalletti,

Vittorio Bianco

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(5), P. e0322960 - e0322960

Published: May 8, 2025

Heavy metal contamination in aquatic environments poses a significant threat to microbial communities, yet the subcellular responses of phytoplankton stress remain poorly understood. In particular, effects heavy exposure on structural and physiological properties diatoms require further investigation. Here, we analyze impact cadmium (Cd) copper (Cu) structures diatom Skeletonema pseudocostatum using holographic tomography. This imaging technique enables detailed visualization quantitative analysis subcomponents, including frustules, protoplasm, vacuoles, chloroplasts, under varying concentrations. The study aims understand changes mean refractive index (RI) concentration (e.g., ratio among cell dry mass its biovolume) as indicators cellular response infer if such can be used sentinel species pollution. Findings indicate that exhibit variations RI internal density when exposed different Lower values observed at higher concentrations, considered sign due cytoplasm extrusion and/or vacuolization. results highlight potential S. bioindicator for monitoring water Moreover, show tomography useful tool non-invasive, high-resolution environmental studies.

Language: Английский

Citations

0
Real-time morphometric analysis of targeted therapy for neuroblastoma cell lines in monolayer and 3D hydrogels using digital holographic microscopy DOI Creative Commons
Sofía Granados-Aparici, Isaac Vieco-Martí, Amparo López-Carrasco

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(11), P. 111231 - 111231

Published: Oct. 23, 2024

Language: Английский

Citations

1
Phase Unwrapping in Digital Holography based on SRDU-Net DOI
Chen Yuan, Quan Wang, Guangming Zhang

et al.

Optics Communications, Journal Year: 2024, Volume and Issue: 573, P. 131055 - 131055

Published: Sept. 1, 2024

Language: Английский

Citations

0
Effect of shear rate on early Shewanella oneidensis bacterial adhesion dynamics monitored by Deep Learning DOI Creative Commons

Lucie Klopffer,

Nicolas Louvet, Simon Becker

et al.

Biofilm, Journal Year: 2024, Volume and Issue: 8, P. 100240 - 100240

Published: Nov. 16, 2024

Understanding pioneer bacterial adhesion is essential to appreciate colonization and consider appropriate control strategies. This entrapment at the wall known be controlled by many physical, chemical or biological factors, including hydrodynamic conditions. However, due nature of early adhesion, i.e. a short dynamic process with low biomass involved, such investigations are challenging. In this context, our study aimed evaluate effect shear rate on dynamics. Firstly, population scale assessing kinetics mechanisms responsible for transfer under rates using time-lapse approach. Secondly, individual scale, implementing an automated image processing method based deep learning track each bacterium wall. Bacterial experiments performed model (

Language: Английский

Citations

0
Confocal Microscopy: Revolutionizing Three-Dimensional Imaging In Biological Research. DOI Open Access
Ashish Dwivedi

African Journal of Biomedical Research, Journal Year: 2024, Volume and Issue: unknown, P. 7662 - 7671

Published: Dec. 16, 2024

Confocal microscopy is a high-resolution imaging technique that uses laser scanning to create sharp, three-dimensional images of biological samples, improving the visualization complex specimens.Confocal has wide range applications including stem cell and biofilm analysis, disease diagnosis etc. owing its ability capture detailed cellular structures, allowing for detection abnormalities in tissue morphology organization.The growing interest this technology underscores importance understanding foundations effectively address challenges further advance applications.This review explores history, principles, confocal biology, highlighting trends, challenges, future advancements technology.

Language: Английский

Citations

0