Cited by Critical perspectives on advancing antibiotic resistant gene (ARG) detection technologies in aquatic ecosystems

Advancing Pathogen Identification: The Role of Digital PCR in Enhancing Diagnostic Power in Different Settings DOI

Alessia Mirabile,

Giuseppe Sangiorgio, Paolo Giuseppe Bonacci

et al.

Diagnostics, Journal Year: 2024, Volume and Issue: 14(15), P. 1598 - 1598

Published: July 25, 2024

Digital polymerase chain reaction (dPCR) has emerged as a groundbreaking technology in molecular biology and diagnostics, offering exceptional precision sensitivity nucleic acid detection quantification. This review highlights the core principles transformative potential of dPCR, particularly infectious disease diagnostics environmental surveillance. Emphasizing its evolution from traditional PCR, dPCR provides accurate absolute quantification target acids through advanced partitioning techniques. The addresses significant impact sepsis diagnosis management, showcasing superior specificity early pathogen identification drug-resistant genes. Despite advantages, challenges such optimization experimental conditions, standardization data analysis workflows, high costs are discussed. Furthermore, we compare various commercially available platforms, detailing their features applications clinical research settings. Additionally, explores dPCR's role water microbiology, wastewater surveillance monitoring waterborne pathogens, underscoring importance public health protection. In conclusion, future prospects including methodological optimization, integration with innovative technologies, expansion into new sectors like metagenomics, explored.

Language: Английский

Citations

Highly Sensitive Detection of the Tetracycline Resistance Gene tetA in Water Supply Systems with an Autocatalytic Deoxyribonucleic Acid–Based Cascade Circuit DOI

Runsheng Wang,

Yu‐Dong Zhou,

WeiYing Li

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 138226 - 138226

Published: April 1, 2025

Language: Английский

Citations

Metagenomic airborne resistome from urban hot spots through the One Health lens DOI

Lucía Maestre‐Carballa,

Vicente Navarro‐López, Manuel Martínez‐García

et al.

Environmental Microbiology Reports, Journal Year: 2024, Volume and Issue: 16(3)

Published: June 1, 2024

Human activities are a significant contributor to the spread of antibiotic resistance genes (ARGs), which pose serious threat human health. These ARGs can be transmitted through various pathways, including air, within context One Health. This study used metagenomics monitor resistomes in urban air from two critical locations: wastewater treatment plant and hospital, both indoor outdoor. The presence cell-like structures was confirmed fluorescence microscopy. metagenomic analysis revealed wide variety high diversity antibiotic-resistant bacteria airborne particles collected. showed higher relative abundances with 32 ARG hits per Gb m

Language: Английский

Citations

A multi-modal deep language model for contaminant removal from metagenome-assembled genomes DOI

Bohao Zou,

Jingjing Wang, Yi Ding

et al.

Nature Machine Intelligence, Journal Year: 2024, Volume and Issue: 6(10), P. 1245 - 1255

Published: Oct. 7, 2024

Language: Английский

Citations

Mapping antimicrobial resistance landscape at a city scale sewage network DOI

Clara Díaz-García, Miquel Sánchez‐Osuna, Albert Serra-Compte

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 974, P. 179127 - 179127

Published: March 26, 2025

Language: Английский

Citations

Development and application of a highly sensitive quadruple droplet digital PCR method for simultaneous quantification of sulfonamide resistance genes DOI

Xirong Yin,

Jiayuan Nie,

Huifang Tian

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: May 21, 2025

Sulfonamide resistance genes (sul genes) have a high detection rate and strong transmissibility. Therefore, there is an urgent need to develop more efficient methods enhance the monitoring of sul genes. Current analytical are insufficient for simultaneous accurate quantification all sulfonamides To overcome this limitation, quadruple method was established by integrating droplet digital PCR (ddPCR) with ratio-based probe-mixing strategy, achieving sensitive sul1, sul2, sul3, sul4 in diverse matrices. Correspondingly, primers probes were meticulously designed rigorously validated, critical parameters ddPCR such as annealing temperature, concentrations systematically optimized. As results, demonstrates excellent sensitivity limits (LOD) ranging from 3.98 6.16 copies/reaction, good repeatability (coefficient variation <25%), adequately meeting requirement quantification. Furthermore, new applied across 115 samples, including human feces, animal-derived foods, sewage surface water, positive rates 100% 99.13% 93.91% 68.70% sul4, concentration non-detection 2.14 × 109 copies/g. In summary, developed has potential serve tool

Language: Английский

Citations

An antimicrobial resistance gene situationer in the backyard swine industry of a Philippine City DOI

Jerouen Paul D. Lumabao,

Maria Catherine B. Otero, Joan T. Acaso

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 31, 2024

Antimicrobial resistance (AMR) as a result of antimicrobial overuse and misuse in agriculture is growing concern, especially the predominant but poorly regulated backyard or smallholder swine farms Philippines. A city-scale surveillance genes (ARGs) was conducted through selected to obtain comprehensive understanding AMR situation sector Davao City, The ARGs encoding four classes (β-lactams: bla

Language: Английский

Citations

Critical perspectives on advancing antibiotic resistant gene (ARG) detection technologies in aquatic ecosystems DOI

Zainab N Nassereddine,

Somie Opara,

Oliver A Coutinho

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 957, P. 177775 - 177775

Published: Nov. 30, 2024

Language: Английский

Citations