Selective microbial attachment to LDPE plastic beads during passage through the wastewater network DOI Creative Commons

Constance L. Tulloch,

B. Cotterell,

Igor Pântea

et al.

Chemosphere, Journal Year: 2024, Volume and Issue: 362, P. 142618 - 142618

Published: June 14, 2024

Urban wastewater treatment plants (WWTP) represent key point-source discharges of microplastics (MP) into the environment, however, little is known about microbial carrying capacity plastics travelling through them. The purpose this study was to quantify number cells that become associated with MP at different locations within a WWTP, and assess differences in microbiome communities. We conducted field experiment incubating low density polyethylene (LDPE) beads WWTP influent effluent, as well tracking free floating during passage from large municipal hospital an urban where they were subsequently recovered. Using two cell counting methods - automated flow cytometric true absolute counts indirect quantification via protein content based on model E. coli we quantified attachment LDPE beads. ranged 350 × 103 cm−2 after incubation 990 influent. 16S rRNA gene amplicon sequencing used determine bacterial community structure plastic-associated microbiomes. Our results showed distinct communities developed following exposure each type. Influent (untreated) LDPE-associated microbiomes dominated by Bacillota whereas microbes attached effluent (tertiary treated) Pseudomonadota. In conclusion, provides clear evidence migrating sewer network rapidly accumulate unique structures varying sewage effluent. These findings demonstrate differential microbiological risk routine those released intermittent combined overflows (CSOs) storm events.

Language: Английский

High-Throughput Qpcr Profiling of Antimicrobial Resistance and Bacterial Loads in Wastewater and Receiving Environments: A Risk Assessment DOI
Reshma Silvester,

Nick Woodhall,

William Nurmi

et al.

Published: Jan. 1, 2025

Wastewater treatment plants (WWTPs) are hot spots for the acquisition and spread of antimicrobial resistance (AMR). This regional-based study quantified antibiotic genes (ARGs), mobile genetic elements (MGEs), bacteria in hospital community-derived wastewater receiving environments, using high-throughput qPCR (HT-qPCR). is first to apply Resistomap's Antibiotic Resistance Gene Index (ARGI) find overall ARG load AMR risk assessment. ARGI WWTPs ranged from 2.0 2.3, indicating higher relative levels than mean European 2.0, but lower global 2.4. The highest diversity abundance ARGs were observed untreated community wastewater. reduction total during (0.2- 2 logs) (0.3-1.5 varied spatio-temporally across WWTPs. Despite a decrease bacterial treated effluents, substantial loads still released into environments. Notably, coastal sediments comparable those wastewater, most shared between highlighting impact discharge on these ecosystems. Sewage outfall exposure increased shellfish, emphasising risks shellfish hygiene. provides evidence inform policymaking, advanced methods combined storm overflow (CSO) management mitigate release, protecting water users food chain.

Language: Английский

Citations

0
High-Throughput qPCR Profiling of Antimicrobial Resistance Genes and Bacterial Loads in Wastewater and Receiving Environments DOI Creative Commons
Reshma Silvester,

Nick Woodhall,

William Nurmi

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 126096 - 126096

Published: March 1, 2025

Wastewater treatment plants (WWTPs) are hot spots for the acquisition and spread of antimicrobial resistance (AMR). This regional-based study quantified antibiotic genes (ARGs), mobile genetic elements (MGEs), bacteria in hospital community-derived wastewater receiving environments, using high-throughput qPCR (HT-qPCR). is first to apply Resistomap's Antibiotic Resistance Gene Index (ARGI) as a standardised metric find overall AMR level across different WWTPs. ARGI WWTPs ranged from 2.0 2.3, indicating higher relative ARG levels than mean European 2.0, but lower global 2.4. The highest diversity abundance ARGs were observed untreated community wastewater. reduction total during (0.2- 2 logs) (0.3-1.5 varied spatio-temporally Despite decrease bacterial treated effluents, substantial loads still released into environments. Notably, coastal sediments comparable those wastewater, most shared between highlighting impact discharge on these ecosystems. Sewage outfall exposure increased shellfish, emphasising risks shellfish hygiene. provides evidence inform policymaking, advanced methods combined sewer overflow (CSO) management mitigate release, protecting water users food chain.

Language: Английский

Citations

0
Bacterial pathogen assemblages on microplastic biofilms in coastal waters DOI
Linus Shing Him Lo,

Richard M. Tong,

Wan Chan

et al.

Marine Pollution Bulletin, Journal Year: 2025, Volume and Issue: 216, P. 117958 - 117958

Published: April 23, 2025

Language: Английский

Citations

0
Guidelines toward ecologically-informed bioprospecting for microbial plastic degradation DOI
Feng Cai, Siqi Jiang, Paul Daly

et al.

Biotechnology Advances, Journal Year: 2025, Volume and Issue: 82, P. 108590 - 108590

Published: April 28, 2025

Language: Английский

Citations

0
Microbial Attachment to Ldpe Plastic Beads During Passage Through the Wastewater Network DOI

Constance L. Tulloch,

B. Cotterell,

Igor Pântea

et al.

Published: Jan. 1, 2024

Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI

Language: Английский

Citations

0
Selective microbial attachment to LDPE plastic beads during passage through the wastewater network DOI Creative Commons

Constance L. Tulloch,

B. Cotterell,

Igor Pântea

et al.

Chemosphere, Journal Year: 2024, Volume and Issue: 362, P. 142618 - 142618

Published: June 14, 2024

Urban wastewater treatment plants (WWTP) represent key point-source discharges of microplastics (MP) into the environment, however, little is known about microbial carrying capacity plastics travelling through them. The purpose this study was to quantify number cells that become associated with MP at different locations within a WWTP, and assess differences in microbiome communities. We conducted field experiment incubating low density polyethylene (LDPE) beads WWTP influent effluent, as well tracking free floating during passage from large municipal hospital an urban where they were subsequently recovered. Using two cell counting methods - automated flow cytometric true absolute counts indirect quantification via protein content based on model E. coli we quantified attachment LDPE beads. ranged 350 × 103 cm−2 after incubation 990 influent. 16S rRNA gene amplicon sequencing used determine bacterial community structure plastic-associated microbiomes. Our results showed distinct communities developed following exposure each type. Influent (untreated) LDPE-associated microbiomes dominated by Bacillota whereas microbes attached effluent (tertiary treated) Pseudomonadota. In conclusion, provides clear evidence migrating sewer network rapidly accumulate unique structures varying sewage effluent. These findings demonstrate differential microbiological risk routine those released intermittent combined overflows (CSOs) storm events.

Language: Английский

Citations

0