Bacillus Amyloliquefaciens IKMM and Zinc Nanoparticles as Biocontrol Candidate Induce the Systemic Resistance by Producing Antioxidants in Tomato Plants Challenged with Early Blight Pathogen

Deleted Journal, Journal Year: 2023, Volume and Issue: 76(1), P. 87 - 103

Published: Oct. 25, 2023

Early blight disease of tomato is a devastating causing severe yield losses. The chemical control this has become challenge due to emerging fungicide resistance in Alternaria solani. present study investigates the biocontrol potential Bacillus amyloliquefaciens IKMM strain and zinc nanoparticles (Zn-NPs) against A. vitro results demonstrated that B. displayed strong mycelial growth inhibition (88.76%) solani followed by control. Various concentrations (0.1 ppm, 1 ppm 10 50 100 ppm) Zn-NPs conventional mancozeb showed considerable reduction (82.98 76.55% respectively) at higher concentration (100 with EC50 (4.56; R2 0.945) for (8.8937; 0.966351). In greenhouse experiments, plants treated strain, lower severity (13.33%, 16.35% 25.58% infected (69.24%) ultimately increased biomass accumulation. flavonoid contents leaves were after 72 h as compared whereas, 48 inoculation, total phenol recorded. antioxidants enzymes viz., peroxidase (PO), phenlylalanine ammonialyase (PAL) polyphenol oxidase (PPO) significant increase inoculation induced accumulation reactive oxygen species (ROS) down-regulated defense related genes (PO, PPO PAL) activating mechanism inoculation. Under field experiment, caused both seasons under natural infection conditions. fruit was relatively than plants. herein presented indicate indigenous along could be considered putative approach combat challenging pathogen will assist develop biofungicides Future research should focused on use combination batter management strategies sustainable agricultural production system.

Language: Английский

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Prospects of Bacillus amyloliquefaciens (MZ945930) Mediated Enhancement of Capsicum annuum L. Plants Under Stress of Alternaria alternata in Terms of Physiological Traits, Thiol Content, Antioxidant Defense, and Phytohormones

Journal of Plant Growth Regulation, Journal Year: 2023, Volume and Issue: 43(9), P. 3265 - 3281

Published: Oct. 10, 2023

Abstract Plants encounter many biotic entities, such as fungi, bacteria, and nematodes, which induce stress that disrupts normal metabolism limits the growth productivity of plants. Currently, use plant growth-promoting bacterial endophytes instead synthetic fungicides is intriguingly eco-friendly. An in vitro vivo antagonistic approach using Bacillus amyloliquefaciens RaSh1 was used to mimic pathogenic effect Alternaria alternata . The results showed B. significantly inhibited fungal vitro. Further, Capsicum annuum L. (pepper plants) were grown subjected inoculation with infected A. alternata, then attributes, photosynthetic pigments, physio-biochemical parameters, level endogenous phytohormones assessed. Under pathogen attack, main responses, length, total fresh dry weights, chlorophylls, reduced, accompanied by increases H 2 O As well, infection pepper caused downregulation hormonal system decreasing gibberellins, indole-3-acetic acid, abscisic well cytokinin concentrations. Although, application, an enhancement growth, proline, thiol content, phenylalanine ammonia-lyase, peroxidase leaves appeared while content decreased. Endogenous found be upregulated -inoculated diseased current study rescued modulating antioxidant defense regulating hormones, could control environmentally friendly manner maintaining sustainable agriculture food security.

Language: Английский

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Plant Growth-Promoting Rhizobacteria Microbial Fertilizer Changes Soils’ Microbial Structure and Promotes Healthy Growth of Cigar Tobacco Plants

Agronomy, Journal Year: 2023, Volume and Issue: 13(12), P. 2895 - 2895

Published: Nov. 24, 2023

Cigar tobacco, an economically important cash crop, holds a substantial role within the fiscal framework of national economy. This however, is characterized by marked vulnerability to pathogenic bacteria, culminating in consequential financial loss throughout its cultivation phase. Plant growth-promoting rhizobacteria (PGPR), salient class advantageous bacterial flora, are recognized for their ability enhance plant growth, inhibit deleterious pathogens, and synthesize compounds that either have direct impact on morphogenesis or activate otherwise ineffectual soil components. Through these mechanisms, PGPR augments soil’s nutritional profile, making it more receptive uptake, thus stimulating vegetative growth. The Bacillus subtilis microbial fertilizer, prime exemplar PGPR, demonstrates not only pathogen-suppressive effect but also induction plant’s innate disease resistance mechanism. bolsters resilience fosters probiotic milieu soil, catalyzes formation agglomerate structures, all which contribute enhanced fertility moisture retention, increased friability, facilitation root expansion. In this study, controlled pot experiment was conducted elucidate mechanism through inter-root probiotics rehabilitate ecosystem foster crop growth cigar tobacco seedlings afflicted with black rot bacteria. Four treatments were instituted, including CK: blank control (no application); A: (Bacillus fertilizer); B: both (the fertilizer together pathogen); C: pathogen). Our empirical findings delineate presence bacteria deteriorates environment, thereby constraining transmutation nutrients subsequent assimilation plants. severely impedes development By contrast, application modified community structure, exhibiting antagonistic interaction indigenous species. Relative CK treatment, found invigorate catalytic conversion enzymes, incrementing peroxidase, acid phosphatase, urease, sucrase activity 12.98%, 19.55%, 13.57%, 17.91%, respectively. Meanwhile, observed ameliorate physicochemical attributes, enhancing available content nitrogen, phosphorus, potassium 4.52%, 6.52%, 15.14%, respectively, along augmentation organic matter 17.33%. fortification properties enrichment fertility, as result application, translated into robust 57.23% enhancement vigor 60.47% extension length seedlings. These amendments subsequently fueled uptick parameters plants, increases height, stem girth, leaf count, maximal dimensions, fresh dry weight tobacco.

Language: Английский

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Identification of the Ilex macrocarpa anthracnose pathogen and the antifungal potential of the cell-free supernatant of Bacillus velezensis against Colletotrichum fioriniae

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: June 20, 2024

Introduction Anthracnose is a significant fungal disease that affects tree growth and development, with Colletotrichum spp. exhibiting host non-specificity targeting various organs, making control challenging. Methods This study aimed to identify the pathogenic species causing anthracnose in Ilex macrocarpa Nanchong, Sichuan Province, screen effective fungicides, particularly biological ones. The pathogen was identified as fioriniae through morphological observation, pathogenicity assays, molecular methods. Three five chemical fungicides were evaluated for their effects on mycelial spore germination rate of pathogen. Results results indicated prochloraz most fungicide, while cell-free supernatant (CFS) Bacillus velezensis had inhibitory effect among fungicides. Transcriptome analysis revealed CFS B. significantly reduced expression genes associated ribosomes, genetic information processing, membrane lipid metabolism, sphingolipid biosynthesis C. . Additionally, glutathione pathway’s genes, including key such GST, GFA, Grx, TRR, POD, induced. Furthermore, 17 MFS transporters 9 ABC increased. Autophagy-related ATGs also affected by CFS. Discussion These findings suggest may inhibit interference cell energy metabolism. provide potential target insights into antifungal mechanism which inhibits

Language: Английский

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Siderophore-producing Bacillus amyloliquefaciens BM3 mitigate arsenic contamination and suppress Fusarium wilt in brinjal plants

Journal of Applied Microbiology, Journal Year: 2023, Volume and Issue: 134(10)

Published: Sept. 22, 2023

Arsenic contamination in agricultural soils poses a serious health risk for humans. Bacteria that produce siderophores, primarily iron acquisition, can be relevant combating arsenic toxicity and simultaneously act as biocontrol agents against plant diseases. We evaluated the bioremediation potential of rhizosphere isolate Bacillus amyloliquefaciens BM3 studied interaction between purified siderophore bacillibactin arsenic.BM3 showed high resistance [MIC value 475 24 mM As(V) As(III), respectively] broad spectrum in-vitro antagonism several phytopathogenic fungi. was identified by biochemical characterization 16S rRNA gene sequencing. Scanning electron microscopy (SEM) analysis revealed increased cell size when grown presence sub-lethal concentrations. Bioremediation assays 74% 88.1% reduction As(III) concentrations, respectively. Genetic determinants (arsC aoxB) antifungal traits (bacAB chiA) were detected PCR. chelating ability bacillibactin, from culture filtrate through spectroscopic data analysis, observed CAS assay fluorescence spectrometry. In-vivo application talc-based formulation brinjal seedlings significant Fusarium wilt disease.Strain B. may useful considered large field trials an alternative to chemical fungicides inhibiting soil borne pathogens.

Language: Английский

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