Targeting Yes‐Associated Protein (YAP) in Breast Cancer: In Silico Molecular Dynamics, Luminescence‐Based In Vitro, and In Vivo Validation of Rauvolfia tetraphylla‐Derived Inhibitors DOI

B. Balavaishnavi,

M. Kamaraj,

T. G. Nithya

et al.

Luminescence, Journal Year: 2024, Volume and Issue: 39(12)

Published: Dec. 1, 2024

ABSTRACT The study aims to elucidate the pharmacological mechanism of Rauvolfia tetraphylla against breast cancer through a comprehensive, multi‐faceted approach. This includes molecular docking, dynamics, and experimental validation. Initial screening via ADME analysis network pharmacology identified key compounds potential targets. Protein–protein interaction (PPI) pinpointed Yes‐associated protein‐1 (YAP) as crucial target. Molecular docking revealed that three compounds—ajmaline, reserpine, serpentine—exhibited strong binding affinities with YAP, scores −6.5 −6.7 kcal/mol. dynamics simulations were conducted assess stability these interactions further. Experimental validation showed R. inhibited cell proliferation, an IC50 348.69 μg/mL, while demonstrating cytoprotective effects on Vero cells (IC50: 1056.23 μg/mL). Migration assays indicated 88.5% reduction in migration, increased ROS levels signaled elevated stress cells. Apoptosis was confirmed by AO/EtBr staining. In vivo DMBA‐induced mouse model significant tumor growth inhibition, supported changes YAP expression histopathological analysis. These findings highlight promising therapeutic candidate cancer, offering insights into its mechanisms for future drug development clinical applications.

Language: Английский

Effect of Germination on the Physicochemical Properties, Functional Groups, Content of Bioactive Compounds, and Antioxidant Capacity of Different Varieties of Quinoa (Chenopodium quinoa Willd.) Grown in the High Andean Zone of Peru DOI Creative Commons
Betsy S. Ramos-Pacheco, David Choque-Quispe, Carlos A. Ligarda-Samanez

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(3), P. 417 - 417

Published: Jan. 27, 2024

Germination is an effective strategy to improve the nutritional and functional quality of Andean grains such as quinoa (Chenopodium Willd.); it helps reduce anti-nutritional components enhance digestibility sensory aspects germinated. This work aimed evaluate effect germination (0, 24, 48, 72 h) on physicochemical properties, content bioactive compounds, antioxidant capacity three varieties quinoa: white, red, black high from Peru. Color, composition, mineral content, phenolic flavonoids, activity were analyzed. Additionally, infrared spectra obtained elucidate structural changes during germination. The results showed color variations significant increases (p < 0.05) in proteins, fiber, minerals, after h germination, attributed activation enzymatic pathways. In contrast, a decrease intensity groups –CH–, –CH2–, C–OH, –OH, C–N. Correlation analysis that flavonoids mainly contributed (r = 0.612). represents promising alternative develop ingredients germinated flour with improved attributes.

Language: Английский

Citations

12
High-frequency ultrasound-assisted drug delivery of chia, cress, and flax conjugated hematite iron oxide nanoparticle for sono-photodynamic lung cancer treatment in vitro and in vivo DOI Creative Commons
Samir Ali Abd El-Kaream,

Doha Farhat Mohamed Zedan,

Hagar Mohamed Mohamed

et al.

Cancer Nanotechnology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 17, 2024

Abstract Background Sono-photodynamic therapy (SPDT), which combines photodynamic (PDT) and sonodynamic (SDT) therapies with sensitizers, offers new avenues for cancer treatment. Even though sensitizers SPDT have been synthesized great success, few of them are effectively used. The limited tumor-targeting specificity, inability to transport the deeply intratumorally, deteriorating tumor microenvironment limit their anti-tumor effectiveness. current study was carried out aiming at high-frequency ultrasound-assisted drug delivery chia, cress flax conjugated hematite iron oxide nanoparticles (CCF–HIONP) photothermal–photodynamic lung (LCA) treatment in vitro vivo as activated up-to-date modality. Materials methods conducted on human LCA cells (A-549) protocol application groups Swiss albino mice treated benzo[a]pyrene only were not received any inducing LCA, after induction began, daily CCF–HIONP HIFU–SPDT sensitizer or without exposure laser (IRL) ultrasound (HIFU–US) a combination and/or 3 min 2 weeks. Results Revealed that HIONP can be employed effective CCF system directly targets cells. In addition, is promising HIFU–SPS when combined very LCA–A549 (cell viability decreased dose-dependent basis, cell cycle progression G0/G1 slowed down, death induced evidenced by an increase population Pre-G cells, early late apoptosis necrosis, autophagic death) LCA-induce (decreased oxidative stress (MDA), ameliorated enzymatic non-enzymatic antioxidants (SOD, GR, GPx, GST, CAT, GSH, TAC) well renal (urea, creatinine) hepatic (ALT, AST) functions, antiproliferative genes (caspase 3,9, p53, Bax, TNFalpha), suppressed antiapoptotic antiangiogenic (Bcl2,VEGF respectively) reducing growth tumors even leading death. This process could attributed photochemical sono-chemical activation mechanism HIFU–SPDT. Conclusions results indicate has promise innovative, selective localized

Language: Английский

Citations

4
Drying Kinetics and Thermodynamic Analysis; Enhancing Quinoa (Chenopodium Quinoa Willd.) Quality Profile Via Pre-Treatments Assisted Germination and Processing DOI

Jabir Khan,

Palwasha Gul,

Qingyun Li

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Drying kinetics and thermodynamic analysis; enhancing quinoa (Chenopodium quinoa Willd.) quality profile via pre-treatments assisted germination and processing DOI Creative Commons

Jabir Khan,

Palwasha Gul,

Qingyun Li

et al.

Ultrasonics Sonochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 107337 - 107337

Published: April 1, 2025

Pre-treatments assisted germination is an efficient technique to enhance the nutritional profile of Quinoa (Chenopodium quinoa Willd.). The present study investigated impact pre-treatments nutritional, anti-nutritional, and structural properties. grains JQ-778 were subjected various including soaking, ultrasound at 28 kHz &40 (US kHz, US 40 kHz) for 30 min followed by over 96-hour 25 °C in a Biochemical-Incubator, 12/12 h dark light dried temperatures 50 °C, 60 70 combined (70 °C). Among evaluated models, page logarithmic showed best fit, presenting highest, R2 ≥ 0.9991, X2 ≤ 0.0013, RMSE 0.0022, RSS 0.0201. Moisture diffusion varied from 3.74 × 10-9 8.36 10-9, with 0.9272 0.9837, energy activation 18.25 28.41 kJ/mol 0.9533-0.9896. significantly lowered drying time without affecting germinated bioactive components or other qualitative factors. Ultrasonic pre-treatment yielded highest antioxidant potency composite index 98.78 %. contentof phytic acid tannin dropped 66.66 82.99 % 31.48 41.60 %, respectively (p < 0.05). Each treatment altered quinoa's quality attributes. Principal Component Analysis revealed significant correlations between analyses, explaining 80.37 variability. intensity functional groups decreased infrared spectra, although transmission signals was greater pretreated samples than control. Scanning electron microscopy analysis extensive fragmentation surface erosion after treatment. Our data suggests that ultrasound-treated may their value, making them suggested source high-protein grains, components, distinct

Language: Английский

Citations

0
Exploring the protective effects of ethanolic extract of Chenopodium quinoa seed (quinoa) on the reproductive alterations of BPA-exposed females in vivo: a preliminary study DOI Creative Commons
Pradip Kumar Jana,

Sananda Sil,

Angshita Ghosh

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)

Published: April 22, 2025

Language: Английский

Citations

0
Harnessing the Potential of Quinoa: Nutritional Profiling, Bioactive Components, and Implications for Health Promotion DOI Creative Commons

Xiaomin Xi,

Guanghe Fan,

Huimin Xue

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(7), P. 829 - 829

Published: July 10, 2024

Quinoa, a globally cultivated "golden grain" belonging to

Language: Английский

Citations

3
Chia Seed (Salvia hispanica) Attenuates Chemically Induced Lung Carcinomas in Rats through Suppression of Proliferation and Angiogenesis DOI Creative Commons
Naglaa A. Ali,

Ghada H. Elsayed,

Safaa H. Mohamed

et al.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(9), P. 1129 - 1129

Published: Aug. 27, 2024

In 2022, 2.5 million cases of lung cancer were diagnosed, resulting in 1.8 deaths. These statistics have motivated us to introduce a new natural product which is feasible therapies. This comprehensive study was performed the effects chia seed extracts (70% ethanol and petroleum ether) on vitro vivo models. The invitro cytotoxicity activity studied cell lines (A549 cells). After 48 h, alcohol ether showed more inhibitory influence (IC50, 16.08, 14.8 µg/mL, respectively) A549 cells compared Dox 13.6 µg/mL). vivo, administration (500 mg/kg/day, orally for 20 weeks) recovered 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced cancer, as significant reduction biomarkers, including relative weight (20.0 13.33%), ICAM(31.73 15.66%), c-MYC (80 96%) MMP9(60 69%) expression genes, improvement these changes observed by histopathological examinations tissues control. Chia seeds fought via suppression proliferation, angiogenesis, inflammation, activation apoptosis. activities may be attributed chemical composition chia, identified LC-Mass, such caffeic acid, vanillic kaempferol-3-O-glucuronide, taxifolin. Finally, we can conclude that an anti-lung effect with good safety margin.

Language: Английский

Citations

1
Potential therapeutic use of millet with broad spectrum anticancer properties: current trends and prospects DOI Creative Commons
Arun Kumar Giri,

Samima Sultana,

Ananya Roy

et al.

Discover Food, Journal Year: 2024, Volume and Issue: 4(1)

Published: Dec. 26, 2024

Language: Английский

Citations

0
Exploring the Health Benefits of Quinoa ( Chenopodium Quinoa Willd.): A Review of the Recent Literature DOI
Stergios Melios, Miriam Clegg, Simona Grasso

et al.

Food Reviews International, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 25

Published: Nov. 11, 2024

Quinoa is a particularly nutrient-rich grain crop, which abundant in micronutrients, fibre, and essential amino acids, making it an excellent staple food source. The current review provides outline of the antioxidant anti-inflammatory properties quinoa critical evaluation health benefits following its consumption focusing on diabetes risk factors, non-alcoholic fatty liver disease, hypertension, cancer risk, body weight regulation obesity, cardiovascular disease factors. has been demonstrated to have as hypoglycaemic effect inducing favourable changes cholesterol, HDL LDL levels, inflammatory markers well humans.

Language: Английский

Citations

0
Targeting Yes‐Associated Protein (YAP) in Breast Cancer: In Silico Molecular Dynamics, Luminescence‐Based In Vitro, and In Vivo Validation of Rauvolfia tetraphylla‐Derived Inhibitors DOI

B. Balavaishnavi,

M. Kamaraj,

T. G. Nithya

et al.

Luminescence, Journal Year: 2024, Volume and Issue: 39(12)

Published: Dec. 1, 2024

ABSTRACT The study aims to elucidate the pharmacological mechanism of Rauvolfia tetraphylla against breast cancer through a comprehensive, multi‐faceted approach. This includes molecular docking, dynamics, and experimental validation. Initial screening via ADME analysis network pharmacology identified key compounds potential targets. Protein–protein interaction (PPI) pinpointed Yes‐associated protein‐1 (YAP) as crucial target. Molecular docking revealed that three compounds—ajmaline, reserpine, serpentine—exhibited strong binding affinities with YAP, scores −6.5 −6.7 kcal/mol. dynamics simulations were conducted assess stability these interactions further. Experimental validation showed R. inhibited cell proliferation, an IC50 348.69 μg/mL, while demonstrating cytoprotective effects on Vero cells (IC50: 1056.23 μg/mL). Migration assays indicated 88.5% reduction in migration, increased ROS levels signaled elevated stress cells. Apoptosis was confirmed by AO/EtBr staining. In vivo DMBA‐induced mouse model significant tumor growth inhibition, supported changes YAP expression histopathological analysis. These findings highlight promising therapeutic candidate cancer, offering insights into its mechanisms for future drug development clinical applications.

Language: Английский

Citations

0