Infectious potential and circulation of SARS-CoV-2 in wild rats
PLoS ONE,
Journal Year:
2025,
Volume and Issue:
20(5), P. e0316882 - e0316882
Published: May 12, 2025
Since
the
beginning
of
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
pandemic,
a
wide
range
animal
species
(pets,
mink…)
have
been
naturally
infected
with
this
betacoronavirus.
The
emergence
new
variants
has
increased
ability
SARS-CoV-2
to
infect
that
were
not
susceptible
“original”
SARS-CoV-2,
such
as
mice
and
rats.
This
work
attempted
evaluate
role
urban
rats
in
transmission
by
combining
surveillance
studies
rat
populations
environments,
vivo
experimental
inoculation
comparative
viral-receptor
interaction
silico
analyses.
We
studied
circulation
wild
Rattus
norvegicus
(n
=
401)
captured
areas
sewage
systems
several
French
cities.
Except
for
3
inconclusive
samples
(2/75
from
Bordeaux
1/261
Lyon)
none
353
sera
tested
showed
anti-SARS-CoV-2
antibodies
microsphere
immunoassay.
However,
negative
virus
neutralisation
assay.
No
viral
RNA
was
detected
all
lungs
collected
401
brown
In
complement,
four
groups
(two
wild-type
colonies,
rattus
,
two
laboratory
strains,
Sprague-Dawley
Wistar)
inoculated
Omicron
BA.5.
At
4
days
post-inoculation,
no
infectious
particles
upper
tract
(URT)
while
at
low
level
only
URT
groups.
addition,
seroconversion
observed
14
after
By
molecular
modelling,
BA.5
receptor
binding
domain
(RBD)
had
lower
affinities
ACE2
than
Homo
sapiens
ACE2.
Based
on
these
results
unable
type
study
different
France
SARS-CoV-2.
Language: Английский
Comparison of Three Commercial ELISA Kits for Detection of Antibodies Against SARS-CoV-2 in Serum Samples from Different Animal Species
Viruses,
Journal Year:
2025,
Volume and Issue:
17(5), P. 716 - 716
Published: May 16, 2025
The
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
caused
the
disease
19
(COVID-19)
pandemic,
significantly
impacting
global
health,
economies,
and
social
stability.
In
February
2020,
first
cases
of
SARS-CoV-2
infections
in
animals
were
documented,
highlighting
potential
risks
posed
by
regular
human-animal
interactions
facilitating
viral
transmission.
consequence,
it
is
essential
to
validate
surveillance
methods
for
animals.
present
study,
101
sera
from
different
animal
species
(36
cats,
41
dogs,
4
ferrets,
10
wild
boar,
6
domestic
goats,
lions)
tested
using
three
ELISA
kits
evaluate
humoral
responses
against
SARS-CoV-2.
results
compared
correlated
with
a
pseudovirus
neutralization
test
(pVNT),
considered
as
reference
assay.
ELISA-1,
targeting
receptor
binding
domain
(RBD)
neutralizing
antibodies
(nAbs)
SARS-CoV-2,
exhibited
highest
diagnostic
performance,
proved
be
reliable
tool
initial
screenings
high-throughput
studies.
contrast,
ELISA-2
(also
RBD
nAbs)
ELISA-3
(targeting
nucleoprotein
antibodies)
demonstrated
lower
sensitivity
detecting
seropositive
Language: Английский