Applied Microbiology,
Journal Year:
2024,
Volume and Issue:
4(4), P. 1534 - 1548
Published: Nov. 27, 2024
The
gut
microbiota
plays
an
essential
role
in
its
host’s
nutrition,
development
and
behavior.
Although
crickets
are
becoming
major
ecosystemic
model
systems
have
important
societal
applications,
such
as
alternative
animal
proteins
or
biocatalysts,
little
is
known
about
their
microbiome
acquisition
how
environmental
factors
shape
this
community.
Therefore,
study,
we
exposed
sand
field
to
soils
with
different
characteristics
microbial
communities
test
the
influence
of
these
on
community
composition.
We
used
16S/18S
rRNA
gene
Illumina
sequencing
analyze
soil
communities,
targeting
three
domains
life,
Archaea,
Bacteria,
Eukaryotes.
Our
results
showed
a
dominance
Mucoromycota
fungi
Bacteroidota
microbiota.
were
unable
retrieve
sufficient
read
numbers
for
Archaea.
Most
taxa
that
identified
can
degrade
soil-derived
complex
organic
matter,
likely
helping
host
digest
food.
had
significant
impact
structure,
supporting
our
assumption
environment
acquisition.
Host
sex
also
community,
possibly
because
female
guts
bigger
mass,
leading
differences
oxygen
concentrations.
In
recent
years,
the
potential
of
insects
has
been
explored
as
a
sustainable
protein
alternative
to
sustain
growing
world
population.
Differences
in
way
are
processed
can
affect
their
proximate
composition
and
digestibility.
This
work
studies
effects
combination
different
types
slaughter
methods
drying
temperatures
on
composition,
organic
matter
digestibility
(OMd),
hydrolysis
degree
(DH/NH2
DH/100
g
DM),
total
(TH)
hygienic
sanitary
study
BSFL
(black
soldier
fly
larvae).
Four
were
used:
frozen
(F),
blanched
+
(B),
Melacide®
(M)
liquid
nitrogen
(N),
each
with
three
(50,
70
90
°C).
A
negative
correlation
between
acid
detergent
fibre
(ADF)
parameters
was
obtained.
°C
most
suitable
temperature,
it
reported
higher
values
(DH,
TH),
resulting
conditions
for
food
use.
Slaughtering
blanching
more
convenient
than
traditional
freezing
or
use
achieve
results.
Animals,
Journal Year:
2024,
Volume and Issue:
14(11), P. 1709 - 1709
Published: June 6, 2024
In
recent
years,
the
potential
of
insects
as
a
sustainable
protein
alternative
to
feed
growing
world
population
has
been
explored.
Differences
in
ways
are
processed
can
affect
their
proximate
composition
and
digestibility.
This
work
studied
effects
combination
different
types
slaughter
methods
drying
temperatures
on
composition,
organic
matter
digestibility
(OMd),
hydrolysis
degree
(DH/NH2
DH/100
g
DM),
total
(TH),
hygienic
sanitary
characteristics
BSFL
(black
soldier
fly
larvae)
meal.
Four
were
used
including
freezing
(F),
blanching
+
(B),
Melacide®
(M),
liquid
nitrogen
(N).
Each
these
was
with
three
(50,
70,
90
°C).
A
negative
correlation
between
acid
detergent
fiber
(ADF)
parameters
obtained.
The
most
suitable
temperature
70
°C,
it
produced
higher
values
(DH
TH),
resulting
conditions
for
food
use.
Slaughtering
more
conducive
achieving
high
results
than
traditional
or
use
Melacide®.
Insects,
Journal Year:
2024,
Volume and Issue:
15(10), P. 734 - 734
Published: Sept. 24, 2024
Corn
straw
is
considered
a
renewable
biomass
energy
source,
and
its
unreasonable
disposal
leads
to
resource
waste
environmental
pollution.
Black
soldier
fly
(Hermetia
illucens
L.)
larvae
(BSFL)
facilitate
the
bioconversion
of
various
types
organic
wastes.
In
this
study,
we
found
that
88%
BSFL
survived,
37.4%
corn
was
digested
after
14
days
feeding
with
straw.
Contrary
expectations,
pretreatment
alkaline
hydrogen
peroxide
did
not
promote
digestion
but
rather
reduced
growth
survival
rates
BSFL.
Acinetobacter,
Dysgonomonas,
unclassified
Enterobacteriaceae
were
abundant
genera
in
gut
fed
Compared
standard
diet,
relative
abundances
carbohydrate
metabolism
genes,
such
as
gene
β-glucosidase
α-glucosidase,
higher
substrate.
These
results
suggested
microbial
community
could
regulate
suitable
functional
microorganisms
response
substrates.
Furthermore,
four
cellulase-producing
strains,
namely
Klebsiella
pneumoniae,
Proteus
mirabilis,
oxytoca,
Providencia
rettgeri,
isolated
from
guts
strains
helped
increase
conversion
straw,
weights
BSFL,
rates.
summary,
reared
discovered
functions
adapting
We
also
declared
benefits
digesting
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 23, 2024
Abstract
Developing
low-cost
media
is
one
of
the
major
challenges
in
cellular
agriculture
domain.
Thus,
this
study
aimed
to
develop
for
cell-cultivated
seafood
using
gut-microbial
community-assisted
fermentation.
Black
soldier
fly
larvae
(
Hermetia
illucens
)
were
used
as
substrate
and
exposed
gut
microbial
communities
isolated
from
Blue
catfish
Ictalurus
furcatus
).
In
first
step,
BSFL
slurry
was
subjected
enzymatic
digestion,
pepsin
trypsin
mimic
animal
digestive
processes.
The
results
showed
a
2.8%
degree
hydrolysis
after
digestion
with
an
additional
5.9%
trypsin.
second
two
fermentation
approaches
tested,
including
direct
addition
homogenate
hydrolysates
(fermentation
A)
establishment
cultures
before
B).
Both
fermentations
resulted
similar
protein
content
hydrolysis.
Fermentation
led
decrease
species
richness,
loss
important
chitinase
protease-producing
genera
such
Pseudomonas
Clostridiaceae.
However,
there
increase
Paraclostridium
members
Enterobacteriaceae.
addition,
effect
fermented
on
proliferation
zebrafish
embryo
fibroblasts
tested
comparison
fetal
bovine
serum
(FBS)
vitro
cell
cultivation.
Lower
concentrations
FBS
decreased
density
altered
morphology.
supplementation
hydrolysate
B
at
high
peptide
had
cytotoxic
effects
cells,
while
lower
concentrations,
it
improved
only
2.5%
FBS.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 24, 2024
Abstract
Background
The
gut
microbiota
of
black
soldier
fly
larvae
(BSFL,
Hermetia
illucens)
play
a
crucial
role
in
recycling
various
organic
waste
streams.
This
capability
is
linked
to
the
presence
potential
common
core
BSFL.
However,
subjective
thresholds
for
defining
taxa
and
difficulty
separating
genetic
environmental
influences
have
prevented
clear
consensus
literature.
We
analysed
bacterial
communities
two
genetically
distinct
BSF
lines
(wild
type
(WT)
lab-adapted
line
(LD))
raised
on
ten
different
diets
based
agricultural
by-products
food
Southeast
Asia.
Results
High-throughput
16S
rRNA
gene
sequencing
revealed
that
were
significantly
influenced
by
genetics
(p
=
0.001),
diet
(plant/meat-based;
p
larval
age
their
interactions
0.002).
led
us
investigate
both
lineage-specific
taxa.
At
strict
>
97%
prevalence
threshold,
four
identified:
Providencia_A_732258,
an
unclassified
genus
within
family
Enterococcaceae,
Morganella,
Enterococcus_H_360604.
A
relaxed
threshold
(>
80%
prevalence)
extended
include
other
such
as
Klebsiella,
Proteus,
Scrofimicrobium.
Our
data
suggest
Scrofimicrobium,
Corynebacterium,
Vagococcus_B,
Lysinibacillus_304693
(all
LD),
Paenibacillus_J_366884
are
members
rather
than
90%
either
LD
or
WT,
with
between
≤
0.05)).
Positive
correlations
observed
several
genera
performance
LD,
typical
highly
optimized
line.
Interestingly,
only
Providencia
appeared
most
aspects
lineages.
Conclusion
study
demonstrates
factors,
composition,
age,
interactions.
identified
microbiota,
emphasizing
background's
role.
Future
studies
should
apply
standardized
high
at
least
unless
there
valid
reason
relaxation
sample
exclusion.
consistent
association
spp.
across
highlights
BSFL
ecosystem.
Applied Microbiology,
Journal Year:
2024,
Volume and Issue:
4(4), P. 1534 - 1548
Published: Nov. 27, 2024
The
gut
microbiota
plays
an
essential
role
in
its
host’s
nutrition,
development
and
behavior.
Although
crickets
are
becoming
major
ecosystemic
model
systems
have
important
societal
applications,
such
as
alternative
animal
proteins
or
biocatalysts,
little
is
known
about
their
microbiome
acquisition
how
environmental
factors
shape
this
community.
Therefore,
study,
we
exposed
sand
field
to
soils
with
different
characteristics
microbial
communities
test
the
influence
of
these
on
community
composition.
We
used
16S/18S
rRNA
gene
Illumina
sequencing
analyze
soil
communities,
targeting
three
domains
life,
Archaea,
Bacteria,
Eukaryotes.
Our
results
showed
a
dominance
Mucoromycota
fungi
Bacteroidota
microbiota.
were
unable
retrieve
sufficient
read
numbers
for
Archaea.
Most
taxa
that
identified
can
degrade
soil-derived
complex
organic
matter,
likely
helping
host
digest
food.
had
significant
impact
structure,
supporting
our
assumption
environment
acquisition.
Host
sex
also
community,
possibly
because
female
guts
bigger
mass,
leading
differences
oxygen
concentrations.
