Some miRNA signatures associated with the use of Etomidate and Propofol and their implications for cardiac surgery

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Background A growing body of evidence indicates that the anesthetics Propofol and Etomidate are associated with neurotoxic effects considerable cognitive impairment. The varying impacts these anesthetic agents on miRNA expression have been validated. Nevertheless, substances cardiovascular system their potential side in humans remain inadequately understood. This study aims to examine impact profiles patients who undergoing surgery. Materials Methods research examined levels miR-21, miR-146, miR-155 blood cells prior following administration Propofol. Furthermore, relationship between specific microRNAs various clinicopathological characteristics. Blood samples were obtained from 60 for each agent at three time points: before surgery, immediately after 24 hours postoperatively. Mononuclear separated utilizing Ficoll reagent, RNA was extracted using Qiazol Real-Time quantitative PCR employed assess levels. primary outcomes measured mortality rates ICU hospital, while secondary encompassed number ICU- hospital-free days observed up seven Results results indicate an increase a decrease miR-21 expression, no change expression. It is worth noting differences statistically significant, variations minor. Within post-surgery, returned baseline level when propofol administered procedure. Additionally, it important mention miR-146 decreased surgery anesthesia, although this not significant. significant moderate inverse correlation discovered expressions miR-146. clinical pathological issues observed, there association differentially expressed genes factors. Conclusion highlights inflammation, heart failure, neurotoxicity, dysfunction, learning difficulties. Based features, discernible preference either or anesthesia. With achieved showing downregulation upregulation miR-146a, interesting monitor over time. underscores importance monitoring function, capabilities

Language: Английский

The circulatory levels and bone expression of MIR21, 34a, 155 and their target genes in a section of Egyptian Population

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 13, 2024

Abstract Bone tissue is constantly regenerated and repaired through a finely balanced process known as bone remodeling. Many miRNAs act regulators of the signaling pathways involved in metabolic processes to maintain homeostasis. This study aimed assess circulating levels MIR21, MIR34a, MIR155 human serum their expression, expression turnover-related genes which can reflect quality. prospective was conducted on 60 patients (30 males 30 females) indicated for surgical interventions neural decompression +/- fixation. Relative quantification miR34a, related assayed using PCR. The osteocalcin Serum Alkaline Phosphatase (sBAP) were ELISA kit. main finding present work strong positive association between only miR21 population under with markers target Also, found both MIR21 age sBAP. These results suggest that these microRNAs early predication

Language: Английский

MicroRNA Frontiers: Illuminating Early Detection Paths in Multiple Sclerosis

Multiple Sclerosis and Related Disorders, Journal Year: 2024, Volume and Issue: 95, P. 106237 - 106237

Published: Dec. 16, 2024

Language: Английский