Aggregation‐Assisted Three‐Photon Fluorescence Resonance Energy Transfer Boosts Phosphorescence for Deep‐Tissue Time‐Resolved Intravital Brain Imaging DOI

Bingshun Wu,

Yifei Li,

Lan Liu

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Abstract Three‐photon phosphorescence microscopic bioimaging holds promise for deep‐tissue time‐resolved brain imaging with high spatial resolution and contrast. However, developing probes bright strong second near‐infrared (NIR‐II) three‐photon absorption suitable biological applications remains a formidable challenge. Herein, kind of fluorescence resonance energy transfer (FRET)‐based nanoparticles (NPFA‐PorPt NPs) is proposed by co‐encapsulation absorbing aggregation‐induced emission luminogen (NPFA), phosphorescent platinum octaethylporphyrin (PorPt) using 1,2‐distearoyl‐sn‐glycero‐3‐phosphoethanolamine‐N‐[methoxy(polyethylene glycol)‐2000] as the encapsulation matrix. NPFA well designed to display superior properties in NIR‐II region its overlaps PorPt, allowing efficient PorPt nanoparticles. The optimal NPFA‐2%PorPt NPs boosted 350‐fold compared that pure aggregates upon same excitation. excited enables be successfully applied vivo muscle vascular deep penetration, resolution, contrast, even small capillaries tissue can recognized. This study paves way development highly multiphoton‐absorbing biomedical applications.

Language: Английский

Strongly absorbing molecules make tissue transparent: a new insight for understanding tissue optical clearing DOI Creative Commons
Tingting Yu, Dan Zhu

Light Science & Applications, Journal Year: 2025, Volume and Issue: 14(1)

Published: Jan. 1, 2025

Abstract Optical imaging plays a central role in the field of biomedicine, but it suffers from light scattering tissues. The research group Stanford University has reported counterintuitive observation that strongly absorbing molecules could achieve optical transparency live animals, providing new insight for understanding tissue clearing. It empowers scientists to leverage techniques vivo wide range deep-seated structures and activities.

Language: Английский

Citations

0
A large field of view 2- and 3-photon microscope DOI Creative Commons
Jack Waters

Light Science & Applications, Journal Year: 2025, Volume and Issue: 14(1)

Published: Feb. 27, 2025

Abstract A new multiphoton fluorescence microscope has been developed, offering cellular resolution across a large field of view deep within biological tissues. This opens possibilities range sciences, particularly neuroscience where optical approaches can reveal signaling in real time throughout an extended network cells distributed through the brain awake, behaving mouse.

Language: Английский

Citations

0
Sidelobe-free deterministic 3D nanoscopy with λ/33 axial resolution DOI Creative Commons

Binxiong Pan,

Baoju Wang,

Yue Ni

et al.

Light Science & Applications, Journal Year: 2025, Volume and Issue: 14(1)

Published: April 21, 2025

Abstract Deterministic three-dimensional (3D) super-resolution microscopy can achieve light-matter interaction in a small volume, but usually with the axial extension distinctly more elongated than lateral one. The isoSTED method combining two opposing objectives and multiple laser beams offer high at λ/12 level, cost of optical system complexity inherent sidelobes. high-order nonlinear effect by multiphoton excitation would benefit to sub-diffraction resolution as well suppress Herein, an easy-to-use, sidelobe-free deterministic 3D nanoscopy resolution, we developed purely physical strategy (UNEx-4Pi) fusion ultrahighly (UNEx) photon avalanching nanoparticles mirror-based bifocal vector field modulation (4Pi). theoretical studies UNEx-4Pi concept showed that main peak fluorescence spot became sharper its large sidelobe height was suppressed increasing nonlinearity. In addition, simplicity robustness were demonstrated utilizing mirror-assisted single-objective self-interference strategy. Experimentally, realized extremely constringent focal without sidelobes observed, achieving up λ/33 (26 nm) using one low-power CW beam. We also ability scheme bioimaging nuclear envelope BSC-1 cells stained imaged 32 nm. proposed will pave way for highly confined space, thereby advancing cutting-edge technologies like sensing, imaging, lithography, data storage.

Language: Английский

Citations

0
Aggregation‐Assisted Three‐Photon Fluorescence Resonance Energy Transfer Boosts Phosphorescence for Deep‐Tissue Time‐Resolved Intravital Brain Imaging DOI

Bingshun Wu,

Yifei Li,

Lan Liu

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Abstract Three‐photon phosphorescence microscopic bioimaging holds promise for deep‐tissue time‐resolved brain imaging with high spatial resolution and contrast. However, developing probes bright strong second near‐infrared (NIR‐II) three‐photon absorption suitable biological applications remains a formidable challenge. Herein, kind of fluorescence resonance energy transfer (FRET)‐based nanoparticles (NPFA‐PorPt NPs) is proposed by co‐encapsulation absorbing aggregation‐induced emission luminogen (NPFA), phosphorescent platinum octaethylporphyrin (PorPt) using 1,2‐distearoyl‐sn‐glycero‐3‐phosphoethanolamine‐N‐[methoxy(polyethylene glycol)‐2000] as the encapsulation matrix. NPFA well designed to display superior properties in NIR‐II region its overlaps PorPt, allowing efficient PorPt nanoparticles. The optimal NPFA‐2%PorPt NPs boosted 350‐fold compared that pure aggregates upon same excitation. excited enables be successfully applied vivo muscle vascular deep penetration, resolution, contrast, even small capillaries tissue can recognized. This study paves way development highly multiphoton‐absorbing biomedical applications.

Language: Английский

Citations

0