Embryo–epithelium interactions during implantation at a glance

Journal of Cell Science, Journal Year: 2017, Volume and Issue: 130(1), P. 15 - 22

Published: Jan. 1, 2017

ABSTRACT At implantation, with the acquisition of a receptive phenotype in uterine epithelium, an initial tenuous attachment embryonic trophectoderm initiates reorganisation epithelial polarity to enable stable embryo and differentiation invasive trophoblasts. In this Cell Science at Glance article, we describe cellular molecular events during phase implantation rodent, drawing on morphological studies both vivo vitro, genetic models. Evidence is emerging for repertoire transcription factors downstream master steroidal regulators estrogen progesterone that coordinate alterations polarity, delivery signals stroma cell death or displacement. We discuss what known interactions occur before considering specific adhesion molecules. compare rodent data our much more limited knowledge human system, where direct mechanistic evidence hard obtain. accompanying poster, represent embryo–epithelium humans laboratory rodents, highlighting similarities differences, as well depict some key biological interstitial occur.

Language: Английский

---

Recurrent pregnancy loss is associated with a pro-senescent decidual response during the peri-implantation window

Communications Biology, Journal Year: 2020, Volume and Issue: 3(1)

Published: Jan. 21, 2020

During the implantation window, endometrium becomes poised to transition a pregnant state, process driven by differentiation of stromal cells into decidual (DC). Perturbations in this process, termed decidualization, leads breakdown feto-maternal interface and miscarriage, but underlying mechanisms are poorly understood. Here, we reconstructed pathway at single-cell level vitro demonstrate that first mount an acute stress response before emerging as DC or senescent (snDC). In absence immune cell-mediated clearance snDC, secondary senescence transforms progesterone-resistant abundantly express extracellular matrix remodelling factors. Additional analysis midluteal identified DIO2 SCARA5 marker genes diverging vivo. Finally, report conspicuous link between pro-senescent peri-implantation recurrent pregnancy loss, suggesting pre-pregnancy screening intervention may reduce burden miscarriage.

Language: Английский

---

Loss of Endometrial Plasticity in Recurrent Pregnancy Loss

Stem Cells, Journal Year: 2015, Volume and Issue: 34(2), P. 346 - 356

Published: Sept. 29, 2015

Menstruation drives cyclic activation of endometrial progenitor cells, tissue regeneration, and maturation stromal which differentiate into specialized decidual cells prior to during pregnancy. Aberrant responsiveness human (HESCs) deciduogenic cues is strongly associated with recurrent pregnancy loss (RPL), suggesting a defect in cellular maturation. MeDIP-seq analysis HESCs did not reveal gross perturbations CpG methylation RPL cultures, although quantitative differences were observed or near genes that are frequently deregulated vivo. However, was marked reduction defined CA-rich motifs located throughout the genome but enriched telomeres. Non-CpG hallmark multipotency. Congruently, we demonstrate deficiency clonogenic cell populations. Loss epigenetic stemness features also correlated intragenic hypomethylation reduced expression HMGB2, coding high mobility group protein 2. We show knockdown this sequence-independent chromatin promotes senescence impairs decidualization, exemplified by blunted time-dependent secretome changes. Our findings indicate stem accelerated limit differentiation capacity endometrium predispose for failure.

Language: Английский

---

Physiologic Events of Embryo Implantation and Decidualization in Human and Non-Human Primates

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(6), P. 1973 - 1973

Published: March 13, 2020

Reproduction is a fundamental process for the preservation of human species. This requires sequence orchestrated events that are necessary successful pregnancy. Two most critical steps in establishment pregnancy implantation and decidualization, which required maternal interactions with developing embryo. review primarily highlights physiological aspects these two adverse outcomes from defective decidualization. The focus this to provide general concept mechanisms involved during window implantation, description components possible pathologies could disrupt embryo decidualization specifically as it applies women non-human primates.

Language: Английский

---

Uterine polyps, adenomyosis, leiomyomas, and endometrial receptivity

Fertility and Sterility, Journal Year: 2019, Volume and Issue: 111(4), P. 629 - 640

Published: March 29, 2019

Language: Английский

---