Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb

eLife, Journal Year: 2018, Volume and Issue: 7

Published: March 29, 2018

During development, coordinated cell behaviors orchestrate tissue and organ morphogenesis. Detailed descriptions of lineages provide a powerful framework to elucidate the mechanisms To study cellular basis limb we imaged transgenic fluorescently-labeled embryos from crustacean Parhyale hawaiensis with multi-view light-sheet microscopy at high spatiotemporal resolution over several days embryogenesis. The lineage outgrowing thoracic limbs was reconstructed single-cell new software called Massive Multi-view Tracker (MaMuT). In silico clonal analyses suggested that early primordium becomes subdivided into anterior-posterior dorsal-ventral compartments whose boundaries intersect distal tip growing limb. Limb-bud formation is associated spatial modulation proliferation, while elongation also driven by preferential orientation divisions along proximal-distal growth axis. Cellular reconstructions were predictive expression patterns development genes including BMP morphogen Decapentaplegic.

Language: Английский

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The wing imaginal disc

Genetics, Journal Year: 2022, Volume and Issue: 220(4)

Published: March 4, 2022

Abstract The Drosophila wing imaginal disc is a tissue of undifferentiated cells that are precursors the and most notum adult fly. first forms during embryogenesis from cluster ∼30 located in second thoracic segment, which invaginate to form sac-like structure. They undergo extensive proliferation larval stages mature ∼35,000 cells. During this time, distinct cell fates assigned different regions, develops complex morphology. Finally, pupal undergoes morphogenetic processes then differentiates notum. While bulk comprises epithelial cells, it also includes neurons glia, associated with tracheal muscle precursor relative simplicity accessibility disc, combined wealth genetic tools available Drosophila, have make premier system for identifying genes deciphering systems play crucial roles animal development. Studies discs made key contributions many areas biology, including patterning, signal transduction, growth control, regeneration, planar polarity, morphogenesis, mechanics.

Language: Английский

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Growth anisotropy of the extracellular matrix shapes a developing organ

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: March 3, 2023

Abstract Final organ size and shape result from volume expansion by growth changes contractility. Complex morphologies can also arise differences in rate between tissues. We address here how differential guides the morphogenesis of growing Drosophila wing imaginal disc. report that 3D morphology results elastic deformation due to anisotropy epithelial cell layer its enveloping extracellular matrix (ECM). While tissue grows plane, bottom ECM occurs is reduced magnitude, thereby causing geometric frustration bending. The elasticity, are fully captured a mechanical bilayer model. Moreover, expression Matrix metalloproteinase MMP2 controls envelope. This study shows controllable constraint whose intrinsic directs developing organ.

Language: Английский

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Regulation of Body Size and Growth Control

Genetics, Journal Year: 2020, Volume and Issue: 216(2), P. 269 - 313

Published: Oct. 1, 2020

Abstract The control of body and organ growth is essential for the development adults with proper size proportions, which important survival reproduction. In animals, adult determined by rate duration juvenile growth, are influenced environment. nutrient-scarce environments in more time needed period can be extended delaying maturation, whereas rapidly completed nutrient-rich conditions. This flexibility requires integration environmental cues developmental signals that govern internal checkpoints to ensure maturation does not begin until sufficient tissue has occurred reach a size. Target Rapamycin (TOR) pathway primary cell-autonomous nutrient sensor, while circulating hormones such as steroids insulin-like factors main systemic regulators animals. We discuss recent findings Drosophila melanogaster showing environment growth-sensing mechanisms, involving TOR other growth-regulatory pathways, converge on insulin steroid relay centers responsible adjusting development, response external addition this, also monitored coordinated whole-body timing through modulation signaling. coordination involves interorgan communication mediated peptide 8 status. Together, these multiple nutritional feed into neuroendocrine hubs controlling signaling, serving at progression toward delayed. review focuses mechanisms conditions modulate size, highlights conserved architecture this system, made prime model understanding

Language: Английский

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Differential lateral and basal tension drive folding of Drosophila wing discs through two distinct mechanisms

Nature Communications, Journal Year: 2018, Volume and Issue: 9(1)

Published: Oct. 30, 2018

Abstract Epithelial folding transforms simple sheets of cells into complex three-dimensional tissues and organs during animal development. has mainly been attributed to mechanical forces generated by an apically localized actomyosin network, however, contributions at basal lateral cell surfaces remain largely unknown. Here we show that a local decrease tension increased tension, but not apical constriction, drive the formation two neighboring folds in developing Drosophila wing imaginal discs. Spatially defined reduction extracellular matrix density results first fold; fluctuations F-actin lead second fold. Simulations using 3D vertex model distinct mechanisms can epithelial folding. Our combination measurements with tissue reveals how modulations surface edge morphological changes.

Language: Английский

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Dynamic changes in epithelial cell packing during tissue morphogenesis

Current Biology, Journal Year: 2021, Volume and Issue: 31(18), P. R1098 - R1110

Published: Sept. 1, 2021

Language: Английский

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Formation of polarized contractile interfaces by self-organized Toll-8/Cirl GPCR asymmetry

Developmental Cell, Journal Year: 2021, Volume and Issue: 56(11), P. 1574 - 1588.e7

Published: April 30, 2021

Interfaces between cells with distinct genetic identities elicit signals to organize local cell behaviors driving tissue morphogenesis. The Drosophila embryonic axis extension requires planar polarized enrichment of myosin-II powering oriented intercalations. Myosin-II levels are quantitatively controlled by GPCR signaling, whereas polarity patterned expression several Toll receptors. How receptors polarize and how this involves GPCRs remain unknown. Here, we report that differential a single receptor, Toll-8, polarizes through binding the adhesion Cirl/latrophilin. Asymmetric Cirl is sufficient enrich myosin-II, localization asymmetric at Toll-8 boundaries. Exploring process dynamically, reveal exhibit mutually dependent in response quantitative differences neighboring cells. Collectively, propose surface protein complex Toll-8/Cirl self-organizes generate interfaces essential for polarization contractility.

Language: Английский

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Regulation of ecdysone production in Drosophila by neuropeptides and peptide hormones

Open Biology, Journal Year: 2021, Volume and Issue: 11(2)

Published: Feb. 1, 2021

In both mammals and insects, steroid hormones play a major role in directing the animal's progression through developmental stages. To maximize fitness outcomes, hormone production is regulated by environmental conditions experienced animal. ecdysone mediates transitions between stages response to factors such as nutrition. These signals are communicated ecdysone-producing gland via action of neuropeptide peptide signalling pathways. While some these pathways have been well characterized, there evidence suggest more than has previously thought function control production, potentially greater range conditions. Here, we review known regulate model genetic insect Drosophila melanogaster , what regarding that trigger Areas for future research highlighted can further contribute our overall understanding complex orchestration environmental, physiological cues together produce functioning adult organism.

Language: Английский

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Apico-basal cell compression regulates Lamin A/C levels in epithelial tissues

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: March 25, 2021

Abstract The levels of nuclear protein Lamin A/C are crucial for mechanotransduction. known to scale with tissue stiffness and extracellular matrix in mesenchymal tissues. But epithelial tissues, where cells lack a strong interaction the matrix, it is unclear how regulated. Here, we show tissues that apico-basal cell compression, independent stiffness. Using genetic perturbations Drosophila compression regulates by deforming nucleus. Further, mammalian cells, deformation modulating phosphorylation at Serine 22, target degradation. Taken together, our results reveal mechanism regulation which could provide key insights understanding mechanotransduction

Language: Английский

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Upregulation of E93 Gene Expression Acts as the Trigger for Metamorphosis Independently of the Threshold Size in the Beetle Tribolium castaneum

Cell Reports, Journal Year: 2019, Volume and Issue: 27(4), P. 1039 - 1049.e2

Published: April 1, 2019

Body size in holometabolous insects is determined by the at which juvenile larva undergoes metamorphosis to pupal stage. To undergo larva-pupa transition, must reach a critical developmental checkpoint, threshold (TS); however, molecular mechanisms through TS cues this transition remain be fully characterized. Here, we use flour beetle Tribolium castaneum characterize underlying entry into metamorphosis. We found that T. reaches beginning of last larval instar, associated with downregulation TcKr-h1 and upregulation TcE93 TcBr-C. Unexpectedly, while there an association between upregulation, it latter constitutes trigger for initiation. In light our results, evaluate interactions control suggest alternative models.

Language: Английский

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