GAF is essential for zygotic genome activation and chromatin accessibility in the early Drosophila embryo

eLife, Journal Year: 2021, Volume and Issue: 10

Published: March 15, 2021

Following fertilization, the genomes of germ cells are reprogrammed to form totipotent embryo. Pioneer transcription factors essential for remodeling chromatin and driving initial wave zygotic gene expression. In Drosophila melanogaster , pioneer factor Zelda is development through this dramatic period reprogramming, known as maternal-to-zygotic transition (MZT). However, it was unknown whether additional were required transition. We identified an maternally encoded MZT, GAGA Factor (GAF). GAF necessary activate widespread remodel accessibility landscape. demonstrated that preferentially controls expression earliest transcribed genes, while genes expressed during activation predominantly dependent on GAF. Thus, progression MZT requires coordination multiple pioneer-like factors, we propose proceeds control gradually transferred from

Language: Английский

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Profiling and functional characterization of maternal mRNA translation during mouse maternal-to-zygotic transition

Science Advances, Journal Year: 2022, Volume and Issue: 8(5)

Published: Feb. 2, 2022

Translational regulation plays an important role in gene expression and function. Although the transcriptional dynamics of mouse preimplantation embryos have been well characterized, global mRNA translation landscape master regulators zygotic genome activation (ZGA) remain unknown. Here, by developing applying a low-input ribosome profiling (LiRibo-seq) technique, we profiled revealed translational during development. We identified marked transition from MII oocytes to zygotes demonstrated that active maternal mRNAs is essential for maternal-to-zygotic (MZT). further showed two factors, Smarcd2 Cyclin T2, whose activated zygotes, are required chromatin reprogramming ZGA, respectively. Our study thus not only filled knowledge gap on mammalian development but also insights into critical function MZT.

Language: Английский

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The landscape of pioneer factor activity reveals the mechanisms of chromatin reprogramming and genome activation

Molecular Cell, Journal Year: 2022, Volume and Issue: 82(5), P. 986 - 1002.e9

Published: Feb. 18, 2022

Language: Английский

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Chromatin expansion microscopy reveals nanoscale organization of transcription and chromatin

Science, Journal Year: 2023, Volume and Issue: 381(6653), P. 92 - 100

Published: July 6, 2023

Nanoscale chromatin organization regulates gene expression. Although is notably reprogrammed during zygotic genome activation (ZGA), the of regulatory factors this universal process remains unclear. In work, we developed expansion microscopy (ChromExM) to visualize chromatin, transcription, and transcription in vivo. ChromExM embryos ZGA revealed how pioneer factor Nanog interacts with nucleosomes RNA polymerase II (Pol II), providing direct visualization transcriptional elongation as string-like nanostructures. Blocking led more Pol particles clustered around Nanog, stalled at promoters Nanog-bound enhancers. This a new model termed "kiss kick", which enhancer-promoter contacts are transient released by elongation. Our results demonstrate that broadly applicable study nanoscale nuclear organization.

Language: Английский

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Single-cell quantification of ribosome occupancy in early mouse development

Nature, Journal Year: 2023, Volume and Issue: 618(7967), P. 1057 - 1064

Published: June 21, 2023

Abstract Translation regulation is critical for early mammalian embryonic development 1 . However, previous studies had been restricted to bulk measurements 2 , precluding precise determination of translation including allele-specific analyses. Here, address this challenge, we developed a novel microfluidic isotachophoresis (ITP) approach, named RIBOsome profiling via ITP (Ribo-ITP), and characterized in single oocytes embryos during mouse development. We identified differential efficiency as key mechanism regulating genes involved centrosome organization N 6 -methyladenosine modification RNAs. Our high-coverage enabled, our knowledge, the first analysis ribosome engagement These led discovery stage-specific zygotic RNAs with ribosomes reduced transcripts exhibiting allele-biased expression. By integrating proteomics data, discovered that occupancy germinal vesicle-stage predominant determinant protein abundance zygote. The Ribo-ITP approach will enable numerous applications by providing high-resolution from ultra-low input samples cells.

Language: Английский

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Comparative proteomic landscapes elucidate human preimplantation development and failure

Cell, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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ZAR1 and ZAR2 are required for oocyte meiotic maturation by regulating the maternal transcriptome and mRNA translational activation

Nucleic Acids Research, Journal Year: 2019, Volume and Issue: 47(21), P. 11387 - 11402

Published: Oct. 8, 2019

Abstract Zar1 was one of the earliest mammalian maternal-effect genes to be identified. Embryos derived from Zar1-null female mice are blocked before zygotic genome activation; however, underlying mechanism remains unclear. By knocking out and its homolog Zar2 in mice, we revealed a novel function these oocyte meiotic maturation. Zar1/2-deleted oocytes displayed delayed resumption polar body-1 emission higher incidence abnormal spindle formation chromosome aneuploidy. The grown Zar1/2-null contained decreased levels many maternal mRNAs reduced level protein synthesis. Key maturation-associated changes failed occur oocytes, including translational activation encoding cell-cycle proteins cyclin B1 WEE2, as well maternal-to-zygotic transition (MZT) licensing factor BTG4. Consequently, mRNA decay impaired MZT abolished. ZAR1/2 bound regulate activity their 3′-UTRs interacted with other proteins, mRNA-stabilizing MSY2 cytoplasmic lattice components. These results countered traditional view that ZAR1 only functions after fertilization highlight previously unrecognized role regulating transcriptome maturing oocytes.

Language: Английский

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Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis

Development, Journal Year: 2019, Volume and Issue: 146(19)

Published: Sept. 30, 2019

ABSTRACT Histone post-translational modifications are key gene expression regulators, but their rapid dynamics during development remain difficult to capture. We applied a Fab-based live endogenous modification labeling technique monitor the changes in histone levels zygotic genome activation (ZGA) living zebrafish embryos. Among various modifications, H3 Lys27 acetylation (H3K27ac) exhibited most drastic changes, accumulating two nuclear foci 64- 1k-cell-stage The elongating form of RNA polymerase II, which is phosphorylated at Ser2 heptad repeats within C-terminal domain (RNAP2 Ser2ph), and miR-430 transcripts were also concentrated closely associated with H3K27ac. When treated α-amanitin inhibit transcription or JQ-1 binding acetyl-reader proteins, H3K27ac still appeared RNAP2 Ser2ph morpholino not foci, suggesting that precedes active ZGA. anticipate method presented here could be variety developmental processes any model non-model organisms.

Language: Английский

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A satellite repeat-derived piRNA controls embryonic development of Aedes

Nature, Journal Year: 2020, Volume and Issue: 580(7802), P. 274 - 277

Published: April 1, 2020

Language: Английский

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Ythdf m6A Readers Function Redundantly during Zebrafish Development

Cell Reports, Journal Year: 2020, Volume and Issue: 33(13), P. 108598 - 108598

Published: Dec. 1, 2020

During the maternal-to-zygotic transition (MZT), multiple mechanisms precisely control massive decay of maternal mRNAs. N

Language: Английский

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