Neuropeptide Bursicon Influences Reproductive Physiology in Tribolium Castaneum DOI Creative Commons
Emmy Li,

Zidan Zhu,

Jingxiu Bi

et al.

Frontiers in Physiology, Journal Year: 2021, Volume and Issue: 12

Published: Oct. 21, 2021

Bursicon is a neuropeptide belonging to the cystine knot family and composed of burs partner (pburs) subunits. It can form heterodimers or homodimers execute different biological functions. regulate cuticle sclerotization wing maturation, whereas bursicon mediate innate immunity midgut stem cell proliferation. A recent study has shown that potentially induces expression vitellogenin (Vg) in black tiger shrimp Penaeus monodon; however, underlying mechanism remains unknown. In this study, we investigated role reproductive physiology red flour beetle, Tribolium castaneum. The knockdown burs, pburs, its receptor T. castaneum rickets (Tcrk) 2-day pupae significantly downregulated levels Vg1, Vg2, Vg (VgR) genes females 3- 5-day post-adult emergence, leading abnormal oocytes with limited content. silencing repressed number eggs laid completely inhibited egg hatch, pburs dramatically decreased laid, hatch rate, offspring larval size, RNA interference (RNAi) effects persisted next generation. Furthermore, insulin/insulin-like signaling/target rapamycin (TOR) signaling encoding insulin (InR), protein kinase B (Akt), TOR, ribosomal S6 (S6K). Most importantly, injection recombinant (r-pburs) was able upregulate Vg, VgR, InR, Akt, S6K, JH synthesis (JHAMT), Methoprene-tolerant (Met), Taiman (Tai) normal rescue VgR RNAi but failed Tcrk females. We infer influence via Tcrk, possibly by mediating juvenile hormone (JH) IIS/TOR pathway genes, thereby regulating reproduction

Language: Английский

Krüppel homolog 1 mediates juvenile hormone action to suppress photoperiodic reproductive diapause-related phenotypes in the female Chrysoperla nipponensis (Neuroptera: Chrysopidae) DOI Creative Commons

Haiyi Huang,

Dandan Li, Minghui Xu

et al.

Journal of Insect Science, Journal Year: 2025, Volume and Issue: 25(2)

Published: March 1, 2025

Abstract Juvenile hormone (JH) has been revealed to be a critical factor in regulating photoperiod reproductive diapause various insect species, however, little information is known about the detailed mechanisms. In this study, we investigated roles of JH signaling green lacewing, Chrysoperla nipponensis (Okamoto), which potentially important biological control predator. Our results showed that short-day condition induces state including synthesis suppression, ovarian development arrest, and triglyceride accumulation. The interference response genes, Krüppel homolog 1 (Kr-h1), females exhibited diapause-related phenotype such as arrest larger storage. Exogenous III suppresses promote inhibit synthesis. However, exogenous fails rescue Kr-h1-silenced phenotype. Accordingly, our demonstrate role Kr-h1 reproduction.

Language: Английский

Citations

0
The role of insulin receptor InR in photoperiod-regulated reproductive diapause of Chrysoperla nipponensis DOI
Xiao Wang, Minghui Xu,

Xue Kong

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 104305 - 104305

Published: March 1, 2025

Language: Английский

Citations

0
Nutritional and hormonal regulation of mitochondrial biogenesis drives fat body remodeling for reproductive competence DOI Creative Commons
Yiying Li,

Mengyao Lang,

Qiongjie He

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Crosstalk between Nutrition, Insulin, Juvenile Hormone, and Ecdysteroid Signaling in the Classical Insect Model, Rhodnius prolixus DOI Open Access
Jimena Leyria, Samiha A. M. Benrabaa, Marcela Nouzová

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 24(1), P. 7 - 7

Published: Dec. 20, 2022

The rigorous balance of endocrine signals that control insect reproductive physiology is crucial for the success egg production. Rhodnius prolixus, a blood-feeding and main vector Chagas disease, has been used over last century as model to unravel aspects metabolism physiology. Our recent work shown nutrition, insulin signaling, two types lipophilic hormones, juvenile hormone (JH) ecdysteroids, are essential successful reproduction in R. prolixus; however, interplay behind these not established. We combination treatments, gene expression analyses, measurements, ex vivo experiments using corpus allatum or ovary, investigate how interaction might define environment results show after blood meal, circulating JH levels increase, process mainly driven through allatoregulatory neuropeptides. In turn, feeds back provide some its own biosynthesis by regulating critical biosynthetic enzymes allatum. Interestingly, also stimulates synthesis release ecdysteroids from ovary. This study highlights complex network that, together, coordinate cycle.

Language: Английский

Citations

17
Role of nuclear protein Akirin in the modulation of female reproduction in Nilaparvata lugens (Hemiptera: Delphacidae) DOI Creative Commons

Feiyan Gou,

Daowei Zhang,

Siqi Chen

et al.

Frontiers in Physiology, Journal Year: 2024, Volume and Issue: 15

Published: July 9, 2024

Introduction: Akirin as a highly conserved transcription factor, exerts profound influence on the growth, development, immune response, and reproductive processes in animals. The brown planthopper (BPH), Nilaparvata lugens , major pest rice production Asia, possesses high capacity, critical factor contributing to reduced yields. aims of this study were demonstrate regulatory role reproduction BPH. Methods: In study, quantitative PCR (qPCR) was used detect mRNA expression genes. RNA interference (RNAi) downregulate gene, sequencing (RNA-seq) screen for differentially expressed genes caused by downregulation. Hormone contents measured with enzyme linked immunosorbent assay (ELISA), protein content evaluated bicinchoninic acid (BCA) method. Results: Using BPH genome data, we screened an gene ( NlAkirin ). An analysis tissue-specific expressions showed that all tissues tested female BPH, but its level highest ovary. After inhibiting females, number eggs laid, hatching rate, ovarioles decreased. Transcriptome performed, following double-stranded treatment. Compared control, which injected GFP RNA, there 438 upregulated 1012 downregulated genes; vitellogenin Vg ) receptor VgR well related target rapamycin (TOR), juvenile hormone (JH), insulin pathways involved synthesis significantly downregulated. As result knockdown, titers JH III Ecdysone (Ecd) unmated females returned normal levels mated females. ovarian both Discussion conclusion: Our results suggest affects regulating TOR, JH, signaling pathways, addition Ecd. findings research provide novel insights into insect capacity.

Language: Английский

Citations

3
Sex-biased juvenile hormone and gene expression underlie sex difference of stress resistance in the American cockroach DOI

Fangfang Liu,

S. Zhang,

Ping Chen

et al.

Journal of Pest Science, Journal Year: 2024, Volume and Issue: unknown

Published: July 17, 2024

Language: Английский

Citations

3
The Molecular Substrates of Insect Eusociality DOI
Sarah D. Kocher, Callum J. Kingwell

Annual Review of Genetics, Journal Year: 2024, Volume and Issue: 58(1), P. 273 - 295

Published: Aug. 15, 2024

The evolution of eusociality in Hymenoptera—encompassing bees, ants, and wasps—is characterized by multiple gains losses social living, making this group a prime model to understand the mechanisms that underlie behavior complexity. Our review synthesizes insights into evolutionary history molecular basis eusociality. We examine new evidence for key hypotheses pathways regulate behaviors, highlighting convergent on shared toolkit includes insulin/insulin-like growth factor signaling (IIS) target rapamycin (TOR) pathways, juvenile hormone ecdysteroid signaling, epigenetic regulation. emphasize how crosstalk among these nutrient-sensing endocrine enables insects integrate external environmental stimuli, including cues, with internal physiology behavior. argue examining as an integrated regulatory circuit exploring architecture evolves alongside can open door understanding origin complex life histories behaviors group.

Language: Английский

Citations

3
Egfr signaling promotes juvenile hormone biosynthesis in the German cockroach DOI Creative Commons
Laurent Li,

Caisheng Zhou,

Yumei Chen

et al.

BMC Biology, Journal Year: 2022, Volume and Issue: 20(1)

Published: Dec. 13, 2022

Abstract Background In insects, an interplay between the activities of distinct hormones, such as juvenile hormone (JH) and 20-hydroxyecdysone (20E), regulates progression through numerous life history hallmarks. As a crucial endocrine factor, JH is mainly synthesized in corpora allata (CA) to regulate multiple physiological developmental processes, including molting, metamorphosis, reproduction. During last century, significant progress has been achieved elucidating signal transduction pathway, while less made dissecting regulatory mechanism biosynthesis. Previous work shown that receptor tyrosine kinase (RTK) signaling biosynthesis both insects mammals. Here, we performed systematic RNA interference (RNAi) screening identify RTKs involved regulating CA adult Blattella germanica females. Results We found epidermal growth factor (Egfr) required for promoting The Egf ligands Vein Spitz activate Egfr, followed by Ras/Raf/ERK signaling, finally activation downstream transcription Pointed (Pnt). Importantly, Pnt induces transcriptional expression two key enzyme-encoding genes pathway: acid methyltransferase (JHAMT) methyl farnesoate epoxidase (CYP15A1). Dual-luciferase reporter assay shows able promoter region Jhamt . addition, electrophoretic mobility shift confirms directly binds − 941~ 886 nt promoter. Conclusions This study reveals detailed molecular Egfr German cockroach, shedding light on intricate regulation during insect development.

Language: Английский

Citations

15
The microRNA pathway core genes are indispensable for development and reproduction in the brown planthopper, Nilaparvata lugens DOI
Ni Wang, Min Chen, Ying Zhou

et al.

Insect Molecular Biology, Journal Year: 2023, Volume and Issue: 32(5), P. 528 - 543

Published: May 10, 2023

MicroRNAs (miRNAs) are small single-stranded non-coding RNAs involved in a variety of cellular events by regulating gene expression at the post-transcriptional level. Several core genes miRNA biogenesis have been reported to participate wide range physiological events, some insect species. However, functional significance pathway Nilaparvata lugens remains unknown. In present study, we conducted systematic characterisation five biogenesis. We first performed spatiotemporal analysis and found that exhibited similar patterns, with high levels eggs relatively transcriptional ovaries fat bodies females. RNA interference experiments showed injecting third-instar nymphs dsRNAs targeting genes, NlAgo1, NlDicer1, NlDrosha resulted mortality rates various degrees body melanism, moulting defects, wing deformities. Further investigations revealed suppression severely impaired ovarian development oocyte maturation, resulting significantly reduced fecundity disruption intercellular spaces between follicle cells. Moreover, profiles miR-34-5p, miR-275-3p, miR-317-3p, miR-14, Let-7-1, miR-2a-3p were altered response knockdown mixture, suggesting they play essential roles miRNA-mediated expression. Therefore, our results provide solid theoretical basis for N. suggest NlDrosha-dependent reproduction this agricultural pest.

Language: Английский

Citations

9
Regulation of forkhead box O transcription factor by insulin signaling pathway controls the reproductive diapause of the lady beetle, Coccinella septempunctata DOI
Junjie Chen, Xiaoxiao Liu,

Penghui Guo

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 258, P. 128104 - 128104

Published: Nov. 17, 2023

Language: Английский

Citations

8