Role of pioneer neurons and neuroblast behaviors on otic ganglion assembly

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 1, 2023

Abstract Cranial ganglia are aggregates of sensory neurons that mediate distinct types sensation. It is little understood how individual coalesce, distribute and shape the ganglion. The statoacoustic ganglion (SAG) displays several lobes spatially arranged to properly connect with hair cells inner ear. To investigate cellular behaviors involved in 3D organization SAG, we use high resolution confocal imaging single cell labeled zebrafish neuroblasts (NB), photoconversion, photoablation genetic perturbations. We find otic NB delaminate out epithelium an EMT-like manner, rearranging apical polarity primary cilia proteins. also show that, once delaminated, migrate directionally actively, requiring RhoGTPases. Interestingly, tracking delaminated reveals coalesce around a small population pioneer SAG neurons. These not from placode origin populate coalescence region before neurogenesis begins. Upon ablation these cells, migratory pathways disrupted and, consequently, affected. Altogether, this work shows for first time role orchestrating development. Summary Statement Little known cranial organize 3D. unveil repertoire behaviours underlying morphogenesis its dependence on relevant

Language: Английский

Pioneer statoacoustic neurons guide neuroblast behaviour during otic ganglion assembly

Development, Journal Year: 2023, Volume and Issue: 150(21)

Published: Nov. 1, 2023

ABSTRACT Cranial ganglia are aggregates of sensory neurons that mediate distinct types sensation. The statoacoustic ganglion (SAG) develops into several lobes spatially arranged to connect appropriately with hair cells the inner ear. To investigate cellular behaviours involved in 3D organization SAG, we use high-resolution confocal imaging single-cell, labelled zebrafish neuroblasts (NBs), photoconversion, photoablation, and genetic perturbations. We show otic NBs delaminate out epithelium an epithelial-mesenchymal transition-like manner, rearranging apical polarity primary cilia proteins. also that, once delaminated, require RhoGTPases order perform active migration. Furthermore, tracking recently delaminated revealed their directed migration coalescence around a small population pioneer SAG neurons. These neurons, not from placode origin, populate region before neurogenesis begins ablation disrupts NB migratory pathways, consequentially affecting shape. Altogether, this work shows for first time role orchestrating development.

Language: Английский