Redox Biology, Journal Year: 2025, Volume and Issue: unknown, P. 103615 - 103615
Published: March 1, 2025
Language: Английский
Development, Journal Year: 2023, Volume and Issue: 150(10)
Published: May 15, 2023
Embryo implantation in humans is interstitial, meaning the entire conceptus embeds endometrium before placental trophoblast invades beyond uterine mucosa into underlying inner myometrium. Once implanted, embryo survival pivots on transformation of an anti-inflammatory bed, termed decidua, under homeostatic control natural killer cells. Here, we examine evolutionary context and elaborate remodelling after conception humans. We also discuss interactions between decidualising that regulate interstitial determine fitness. Together, this Review highlights precarious but adaptable nature process.
Language: Английский
Citations
52
Nature Genetics, Journal Year: 2024, Volume and Issue: 56(9), P. 1925 - 1937
Published: Aug. 28, 2024
The complex and dynamic cellular composition of the human endometrium remains poorly understood. Previous endometrial single-cell atlases profiled few donors lacked consensus in defining cell types. We introduce Human Endometrial Cell Atlas (HECA), a high-resolution reference atlas (313,527 cells) combining published new transcriptomics datasets 63 women with without endometriosis. HECA assigns identifies previously unreported types, mapped situ using spatial validated independent single-nuclei dataset (312,246 nuclei, donors). In functionalis, we identify intricate stromal-epithelial coordination via transforming growth factor beta (TGFβ) signaling. basalis, define signaling between fibroblasts an epithelial population expressing progenitor markers. Integration large-scale endometriosis genome-wide association study data pinpoints decidualized stromal cells macrophages as most likely dysregulated is valuable resource for studying physiology disorders, guiding microphysiological vitro systems development.
Language: Английский
Citations
30
Frontiers in Medicine, Journal Year: 2022, Volume and Issue: 9
Published: April 28, 2022
Endometriosis is a gynecological disease characterized by the growth of endometrial glands and stroma outside uterine cavity. The incidence very high, there are currently no reliable early diagnostic tests, therapies only symptomatic and, consequently, social impact endometriosis important, also considering related fertility problems. Despite this, pathogenesis still not fully defined. Retrograde menstruation coelomic metaplasia most recognized pathogenetic hypotheses. Recent experimental evidences generated our research group others have indicated an alteration fine-tuning female genital system developmental program during critical window time in fetal life as event prompting to development later life. Goal this article present revision recent literature about different mechanisms proposed for with particular emphasis on embryologic theory. possible clinical pathological implications these findings will be discussed.
Language: Английский
Citations
54
Frontiers in Reproductive Health, Journal Year: 2022, Volume and Issue: 3
Published: Jan. 20, 2022
The human endometrium is a remarkable tissue, undergoing ~450 cycles of proliferation, differentiation, shedding (menstruation), repair, and regeneration over woman's reproductive lifespan. Post-menstrual repair an extremely rapid scar-free process, with re-epithelialization the luminal epithelium completed within 48 h initiation shedding. Following menstruation, functionalis grows from residual basalis layer during proliferative phase under influence rising circulating estrogen levels. regenerative capacity attributed to stem/progenitor cells which reside in both epithelial stromal cell compartments layer. Finding definitive marker for endometrial progenitors (eEPCs) has proven difficult. A number different markers have been suggested as putative progenitor including, N-cadherin, SSEA-1, AXIN2, SOX-9 ALDH1A1, some show functional stem activity vitro assays. Each unique location(s) glandular epithelium, led suggestion that differentiation hierarchy exists, base glands lining functionalis, where express combinations they differentiate move up gland into away niche. Perivascular mesenchymal (eMSCs) can be identified by co-expression PDGFRβ CD146 or single marker, SUSD2. This review will detail known markers; their identity, location interactions across menstrual cycle, particular post-menstrual estrogen-driven regeneration, well possible contributions menstruation-related disorders such endometriosis regeneration-related disorder Asherman's syndrome. We also highlight new techniques allow greater understanding cells' role including 3D organoids, slice cultures gene sequencing at level. Since mouse models are commonly used study we investigated vivo.
Language: Английский
Citations
47
Nature Reviews Endocrinology, Journal Year: 2022, Volume and Issue: 18(12), P. 727 - 743
Published: Sept. 1, 2022
Language: Английский
Citations
45
Journal of Obstetrics and Gynaecology Research, Journal Year: 2023, Volume and Issue: 49(4), P. 1090 - 1105
Published: Feb. 6, 2023
Abstract Endometriosis is a serious, chronic disorder where endometrial tissue grows outside the uterus, causing severe pelvic pain and infertility. It affects 11% of women. multifactorial unclear etiology, although retrograde menstruation plays major role. has genetic component with over 40 risk factors mapped, their mechanism action still emerging. New evidence suggests role for stem/progenitor cells, now that identifying markers these cells are available. Recent lineage tracing clearing microscopy 3D reconstruction provided new understanding glandular structure, particularly horizontal orientation interconnection basalis glands. sequencing technologies, whole genome DNA revealing somatic mutations, including in cancer driver genes, normal eutopic endometrium patients endometriosis, as well ectopic endometriotic lesions. Methylome offering insight into regulation genes environmental factors. Single cell RNA reveals transcriptome individual shedding light on diversity range cellular subpopulations types present epithelial organoid cultures replicating epithelium providing tractable models studying endometriosis. Organoids derived from menstrual fluid offer non‐invasive source avenue testing drugs developing personalized medicine treating These approaches rapidly advancing our endometriosis etiology.
Language: Английский
Citations
33
Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 184, P. 144 - 155
Published: July 2, 2024
Language: Английский
Citations
11
Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 14
Published: Jan. 25, 2024
The endometrium is a resilient and highly dynamic tissue, undergoing cyclic renewal in preparation for embryo implantation. Cyclic endometrial regeneration depends on the intact function of several cell types, including parenchymal, endothelial, immune cells, as well adult stem cells that can arise from or extrauterine sources. ability to undergo rapid, repeated without scarring unique this tissue. However, if tissue process disrupted dysfunctional, women may present clinically with infertility due persistent atrophic/thin endometrium. Such disorders are rate-limiting treatment female success vitro fertilization because dearth options specifically targeting A growing number studies have explored potential mesenchymal (MSCs), treat regeneration. MSCs multipotent capacity differentiate into such adipocytes, chondrocytes, osteoblasts. In addition their differentiation capacity, migrate toward injured sites where they secrete bioactive factors (e.g. cytokines, chemokines, growth factors, proteins extracellular vesicles) aid repair. These modulate biological processes critical regeneration, angiogenesis, migration immunomodulation. MSC secretome has therefore attracted significant attention its therapeutic potential. uterus, utilizing rodent models limited human trials shown benefit infertility. This review will explore impaired receptivity thin scarring. We provide context supporting leveraging purpose by mechanisms which promotes repair nonreproductive tissues.
Language: Английский
Citations
10
Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)
Published: Jan. 2, 2025
Language: Английский
Citations
1
F&S Reviews, Journal Year: 2024, Volume and Issue: 5(3), P. 100075 - 100075
Published: May 24, 2024
Research into menstrual fluid (MF), also referred to as blood or effluent in the literature, and its potential a diagnostic tool has been largely overlooked. The current understanding of endometrium principally derived from invasive techniques, such endometrial biopsies, yet tissue is cyclically shed MF. Menstrual composition that distinctly reflects environment provides an easily accessible source constituents can be collected relatively noninvasively using cup. offers renewable inexpensive option replace more uncomfortable methods collecting samples. health provide invaluable insight development disorders. recently utilized research pathogenesis endometriosis, chronic endometritis, unexplained infertility; however, some logistical challenges recruitment participants, collection, processing MF samples require optimization. This review detailed what we currently know about recent advances research, well future directions. unique window endometrium, further could help uncover disorders have exciting prospects tool.
Language: Английский
Citations
6