Atrial Appendage-Derived Cardiac Micrografts: An Emerging Cellular Therapy for Heart Failure DOI
Esko Kankuri,

Pasi Karjalainen,

Antti Vento

et al.

Published: Jan. 1, 2023

Cells capable of cardiomyocyte-like differentiation have been in the limelight for several years. Such cells investigated extensively their potential to regenerate injured heart when transplanted. The heart's capacity repair is limited, although some degree cardiomyocyte regeneration has shown occur throughout adulthood. However, elusive nature adult cardiac stem led inconclusive evidence presence. Direct replacement and delivery electrically active, contractile are presented with further challenges related electrical coupling arrhythmias. Furthermore, cell types or progenitor characteristics require extensive manipulation, enrichment, expansion before use as therapy. As tissue a finely orchestrated interplay types, it not surprising that combining endothelial mesenchymal promise over therapy using single type. An alternative mechanism direct cardiomyocytes lost to, example ischemic damage, stimulation myocardium's inherent protective capacities pathways functional repair. This achievable by means, including drugs, biomaterials, released soluble factors. In these approaches, lie targeted long-term efficacy therapies. A recent advance utilization an individual's autologous, processed micrografts derived from atrial appendages. appendages pouch-shaped annexes atria. For example, left appendage can contribute formation blood clots, especially during fibrillation, which rationale its clinical closure even excision cases. Hence, attractive strategy obtain safely directly patient. Micrograft transplants produced comprehensively harbor most myocardial intricately meshed supporting extracellular matrix. microtissue produce paracrine factors, such natriuretic peptides, which, therapeutic viewpoint, elucidated convey both antifibrotic cardioprotective effects on myocardium. Their upon open surgery epicardial transplantation, they apt targets gene applications. chapter provides insight into current state autologous micrograft therapy, proposed mechanisms action, means processing transplantation surgery.

Language: Английский

Harnessing developmental dynamics of spinal cord extracellular matrix improves regenerative potential of spinal cord organoids DOI
Zheng Sun,

Zhenni Chen,

Man Yin

et al.

Cell stem cell, Journal Year: 2024, Volume and Issue: 31(5), P. 772 - 787.e11

Published: April 1, 2024

Language: Английский

Citations

22
The PD-1–PD-L1 pathway maintains an immunosuppressive environment essential for neonatal heart regeneration DOI
Stephanie Vargas Aguilar, Miao Cui, Wei Tan

et al.

Nature Cardiovascular Research, Journal Year: 2024, Volume and Issue: 3(3), P. 389 - 402

Published: March 1, 2024

Language: Английский

Citations

4
Blueprints for healing: central nervous system regeneration in zebrafish and neonatal mice DOI Creative Commons
Brianna R. Cellini,

Sreeparvathy Vayankara Edachola,

Timothy D. Faw

et al.

BMC Biology, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 30, 2025

In adult mammals, including humans, neurons, and axons in the brain spinal cord are inherently incapable of regenerating after injury. Studies animals with innate capacity for regeneration providing valuable insights into mechanisms driving tissue healing. The aim this review is to summarize recent data on zebrafish neonatal mice. We infer that elucidating these understanding how why they lost mammals will contribute development strategies promote central nervous system regeneration.

Language: Английский

Citations

0
PI3K-Akt signalling regulates Scx-lineage tenocytes and Tppp3-lineage paratenon sheath cells in neonatal tendon regeneration DOI Creative Commons
Atsushi Goto, Shingo Komura, Koki Kato

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 20, 2025

Tendon injuries are frequently occurring disorders; it is clinically important to enhance tendon regeneration and prevent functional impairment post-injury. While in children heal quickly with minimal scarring, those adults slowly accompanied by fibrotic scarring. Therefore, investigating the healing mechanisms after injury, identifying factors that regulate inherent regenerative capacity of tendons promising approaches promoting regeneration. Here, we identify PI3K-Akt signalling pathway preferentially upregulated injured neonatal murine Achilles tendons. Inhibition a rupture model decreases cell proliferation migration both Scx-lineage intrinsic tenocytes Tppp3-lineage extrinsic paratenon sheath cells. Moreover, inhibition stemness promotes mature tenogenic differentiation Scx- Collectively, these results suggest plays pivotal role

Language: Английский

Citations

0
Enduring questions in regenerative biology and the search for answers DOI Creative Commons
Ashley W. Seifert, Elizabeth M. Duncan, Ricardo M. Zayas

et al.

Communications Biology, Journal Year: 2023, Volume and Issue: 6(1)

Published: Nov. 9, 2023

Abstract The potential for basic research to uncover the inner workings of regenerative processes and produce meaningful medical therapies has inspired scientists, clinicians, patients hundreds years. Decades studies using a handful highly model organisms have significantly advanced our knowledge key cell types molecular pathways involved in regeneration. However, many questions remain about how unfold regeneration-competent species, they are curtailed non-regenerative organisms, might be induced (or restored) humans. Recent technological advances genomics, biology, computer science, bioengineering, stem hold promise collectively provide new experimental evidence different accomplish process In theory, this should inform design clinical approaches medicine. A deeper understanding tissues organs regenerate will also undoubtedly impact adjacent scientific fields. To best apply adapt these technologies ways that break long-standing barriers answer critical regeneration, we must combine deep developmental evolutionary biologists with hard-earned expertise scientists mechanistic technical end, perspective is based on conversations from workshop organized at Banbury Center, during which diverse cross-section regeneration community experts various discussed enduring biology. Here, share group identified as significant unanswered, i.e., known unknowns. We describe obstacles limiting progress answering expanding number diversity used essential deepening capacity. Finally, propose investigating problems collaboratively across network researchers advance field unexpected insights into important related areas biology

Language: Английский

Citations

8
The characteristics of proliferative cardiomyocytes in mammals DOI
Xinyue Yang, Liangpeng Li, Chunyu Zeng

et al.

Journal of Molecular and Cellular Cardiology, Journal Year: 2023, Volume and Issue: 185, P. 50 - 64

Published: Nov. 1, 2023

Language: Английский

Citations

4
Long-term Mouse Spinal Cord Organotypic Slice Culture as a Platform for Validating Cell Transplantation in Spinal Cord Injury DOI Open Access
Francesca Merighi, Sara De Vincentiis, Marco Onorati

et al.

Journal of Visualized Experiments, Journal Year: 2024, Volume and Issue: 206

Published: April 12, 2024

Resolutive cures for spinal cord injuries (SCIs) are still lacking, due to the complex pathophysiology. One of most promising regenerative approaches is based on stem cell transplantation replace lost tissue and promote functional recovery. This approach should be further explored better in vitro ex vivo safety efficacy before proceeding with more expensive time-consuming animal testing. In this work, we show establishment a long-term platform mouse (SC) organotypic slices transplanted human neural cells test cellular replacement therapies SCIs. Standard SC cultures maintained around 2 or 3 weeks vitro. Here, describe an optimized protocol maintenance (≥30 days) up 90 days. The medium used culturing was also transplanting into model. Human SC-derived neuroepithelial (h-SC-NES) carrying green fluorescent protein (GFP) reporter were slices. Thirty days after transplant, GFP expression low apoptotic rate, suggesting that environment sustained their survival integration inside tissue. represents robust reference efficiently testing will allow researchers perform pre-screening different therapies, helping them choose appropriate strategy experiments.

Language: Английский

Citations

1
JoVE Video Dataset DOI Open Access
Francesca Merighi, Sara De Vincentiis, Marco Onorati

et al.

Published: April 13, 2024

Resolutive cures for spinal cord injuries (SCIs) are still lacking, due to the complex pathophysiology. One of most promising regenerative approaches is based on stem cell transplantation replace lost tissue and promote functional recovery. This approach should be further explored better in vitro ex vivo safety efficacy before proceeding with more expensive time-consuming animal testing. In this work, we show establishment a long-term platform mouse (SC) organotypic slices transplanted human neural cells test cellular replacement therapies SCIs. Standard SC cultures maintained around 2 or 3 weeks vitro. Here, describe an optimized protocol maintenance (≥30 days) up 90 days. The medium used culturing was also transplanting into model. Human SC-derived neuroepithelial (h-SC-NES) carrying green fluorescent protein (GFP) reporter were slices. Thirty days after transplant, GFP expression low apoptotic rate, suggesting that environment sustained their survival integration inside tissue. represents robust reference efficiently testing will allow researchers perform pre-screening different therapies, helping them choose appropriate strategy experiments.

Language: Английский

Citations

0
Suppressing DBNDD2 promotes neuron growth and axon regeneration in adult mammals DOI Creative Commons
Lan Zhang, Wei Dai,

Yucong Wu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 23, 2024

Abstract Effective axon regeneration is essential for the successful recovery of nerve functions in patients with injury-associated neurological diseases. Certain self-regeneration occurs injured peripheral axonal branches dorsal root ganglion (DRG) neurons but does not occur their central branches. By performing rat sciatic or axotomy, we determined expression dysbindin domain containing 2 (DBNDD2) DRGs after regenerative injury non-regenerative injury, respectively, and found that DBNDD2 down-regulated up-regulated injury. Moreover, differs neonatal adult gradually increased during development. knockdown promotes outgrowth neurites both DRG stimulates robust rats crush Bioinformatic analysis data showed transcription factor estrogen receptor 1 (ESR1) interacts DBNDD2, exhibits a similar trend as may targets DBDNN2. These studies indicate reduced level low abundance neonates contribute to thus suggest manipulation promising therapeutic approach improving damage.

Language: Английский

Citations

0
High-Resolution Spatiotemporal Transcriptomic Atlas Reveals Ly6a+ Peritenon Stem Cell Population and FGF7 Signaling are Critical for Tendon Formation DOI

Tingyun Lei,

Ruifu Lin,

Jie Han

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0