Rho/Rok-dependent regulation of actomyosin contractility at tricellular junctions restricts epithelial permeability in Drosophila
Current Biology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 1, 2025
Language: Английский
The dynamics of tubulogenesis in development and disease
Development,
Journal Year:
2025,
Volume and Issue:
152(3)
Published: Feb. 1, 2025
ABSTRACT
Tubes
are
crucial
for
the
function
of
many
organs
in
animals
given
their
fundamental
roles
transporting
and
exchanging
substances
to
maintain
homeostasis
within
an
organism.
Therefore,
development
maintenance
these
tube-like
structures
is
a
vital
process.
can
form
diverse
ways,
advances
our
understanding
molecular
cellular
mechanisms
underpinning
different
modes
tubulogenesis
have
significant
impacts
biological
contexts,
including
disease.
This
Review
discusses
recent
progress
developmental
underlying
tube
formation.
Language: Английский
Neighbor cells restrain furrowing during Xenopus epithelial cytokinesis
Developmental Cell,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 1, 2025
Language: Английский
Vertex remodeling during epithelial morphogenesis
Current Opinion in Cell Biology,
Journal Year:
2024,
Volume and Issue:
91, P. 102427 - 102427
Published: Sept. 26, 2024
Epithelial
cells
adhere
to
each
other
via
intercellular
junctions
that
can
be
classified
into
bicellular
and
tricellular
contacts
(vertices).
morphogenesis
involves
cell
rearrangement
requires
remodeling
of
vertices.
Although
our
understanding
how
junction
mechanics
drive
epithelial
has
advanced,
the
mechanisms
underlying
vertex
during
this
process
have
only
received
attention
recently.
In
review,
we
outline
recent
progress
in
reorganize
adhesion
cytoskeleton
trigger
displacement
resolution
We
will
also
discuss
achieve
optimal
balance
between
structural
flexibility
stability
their
Finally,
introduce
new
modeling
frameworks
designed
analyze
at
Integration
live
imaging
techniques
is
providing
insights
active
roles
vertices
morphogenesis.
Language: Английский
Rho/Rok-dependent regulation of actomyosin contractility at tricellular junctions controls epithelial permeability in Drosophila
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 5, 2024
Summary
Cell
contacts
in
epithelia
are
remodeled
to
regulate
paracellular
permeability
and
control
passage
of
migrating
cells,
but
how
barrier
function
is
modulated
while
preserving
epithelial
integrity
not
clear.
In
the
follicular
epithelium
Drosophila
ovaries,
tricellular
junctions
(TCJs)
open
transiently
a
process
termed
patency
allow
externally
produced
yolk
proteins
for
uptake
by
oocyte.
Here
we
show
that
modulation
actomyosin
contractility
at
cell
vertices
controls
TCJ
permeability.
Before
patency,
circumferential
bundles
anchored
apical
follicle
vertices,
where
tension-sensing
junctional
proteins,
Rho-associated
kinase
(Rok),
active
Myosin
II
accumulate
maintain
closed.
opening
initiated
redistribution
from
medial
pool,
accompanied
decreasing
tension
on
vertices.
This
transition
requires
activation
Cofilin-dependent
F-actin
disassembly
phosphatase
Slingshot
inactivation
light
chain
phosphatase,
counteracted
Rok.
Accordingly,
constitutive
or
Rho
signaling
prevent
vertex
opening,
whereas
reduced
Rok
activity
cause
excessive
premature
opening.
Thus,
intercellular
gaps
does
require
actomyosin-based
forces,
relies
reduction
contractility.
Conversely,
assembly
required
closing
after
patency.
Our
findings
consistent
with
force
transduction
model
which
maintained
vertex-anchored
contractile
actomyosin.
We
propose
cell-type-specific
organization
determines
mode
contractility-dependent
regulation
Language: Английский