Morphogens in the evolution of size, shape and patterning DOI Creative Commons
Lewis Scott Mosby,

Amy E. Bowen,

Zena Hadjivasiliou

et al.

Development, Journal Year: 2024, Volume and Issue: 151(18)

Published: Sept. 15, 2024

Much of the striking diversity life on Earth has arisen from variations in way that same molecules and networks operate during development to shape pattern tissues organs into different morphologies. However, we still understand very little about potential for diversification exhibited by different, highly conserved mechanisms evolution, or, conversely, constraints they place evolution. With aim steering field new directions, focus morphogen-mediated patterning growth as a case study demonstrate how developmental can adapt evolution drive morphological optimise functionality, illustrate algorithms computational tools be used alongside experiments provide insights these evolve. We first introduce key properties morphogen-driven mechanisms, before summarising comparative studies exemplify changes spatiotemporal expression signalling levels morphogens impact organ size, nature. Finally, detail theoretical frameworks conjunction with probe role conclude is an excellent model system offers generally applicable framework investigate mechanisms.

Language: Английский

Butterfly pupal wing tissue with an eyespot organizer DOI Creative Commons
Joji M. Otaki, Atsuko Tanaka,

Euichi Hirose

et al.

Cells and Development, Journal Year: 2025, Volume and Issue: unknown, P. 203992 - 203992

Published: Jan. 1, 2025

Language: Английский

Citations

2
A long noncoding RNA at the cortex locus controls adaptive coloration in butterflies DOI Creative Commons
Luca Livraghi, Joseph J. Hanly,

Elizabeth Evans

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(36)

Published: Aug. 30, 2024

Evolutionary variation in the wing pigmentation of butterflies and moths offers striking examples adaptation by crypsis mimicry. The

Language: Английский

Citations

7
Lepidopteran scale cells derive from sensory organ precursors through a canonical lineage DOI Creative Commons
Ling S. Loh, Kyle A. DeMarr, Christa Heryanto

et al.

Development, Journal Year: 2025, Volume and Issue: 152(5)

Published: March 1, 2025

The success of butterflies and moths is tightly linked to the origin scales within group. A long-standing hypothesis postulates that are homologous well-described mechanosensory bristles found in fruit fly Drosophila melanogaster, as both derive from an epithelial precursor. Previous histological candidate gene approaches identified parallels genes involved scale bristle development. Here, we provide developmental transcriptomic evidence differentiation lepidopteran derives sensory organ precursor (SOP). Live imaging pupae shows SOP cells undergo two asymmetric divisions first abrogate neurogenic lineage, then lead a differentiated its associated socket cell. Single-nucleus RNA sequencing using early pupal wings revealed differential expression patterns mirror development, suggesting shared program. Additionally, recovered newly gene, transcription factor pdm3, proper butterfly wing scales. Altogether, these data open up avenues for understanding type specification illustrate how single-cell transcriptomics powerful platform evolution cell types.

Language: Английский

Citations

1
Spatial and temporal regulation of Wnt signaling pathway members in the development of butterfly wing patterns DOI Creative Commons
Tirtha Das Banerjee, Suriya Narayanan Murugesan, Heidi Connahs

et al.

Science Advances, Journal Year: 2023, Volume and Issue: 9(30)

Published: July 26, 2023

Wnt signaling members are involved in the differentiation of cells associated with eyespot and band color patterns on wings butterflies, but identity spatio-temporal regulation specific pathway remains unclear. Here, we explore localization function Armadillo/β-catenin dependent (canonical) independent (noncanonical) development

Language: Английский

Citations

17
A long non-coding RNA at thecortexlocus controls adaptive colouration in butterflies DOI Creative Commons
Luca Livraghi, Joseph J. Hanly,

Elizabeth Evans

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 12, 2024

Abstract Evolutionary variation in the wing pigmentation of butterflies and moths offers striking examples adaptation by crypsis mimicry. The cortex locus has been independently mapped as controlling colour polymorphisms 14 lepidopteran species, suggesting it acts a genomic hotspot for diversification patterns, but functional validation through protein-coding knockouts proven difficult to obtain. Our study unveils role novel long non-coding RNA (lncRNA) which we name ivory , transcribed from locus, modulating patterning butterflies. Strikingly, expression prefigures most melanic patterns during pupal development, an early developmental specifying scale identity. To test this, generated CRISPR mosaic knock-outs five nymphalid butterfly species show that mutagenesis yields transformations dark pigmented scales into white or light-coloured scales. Genotyping Vanessa cardui germline mutants associates these phenotypes small on-target deletions at conserved first exon . In contrast, mutant with confirmed null alleles lack any phenotype, exclude this adjacent gene. Overall, results lncRNA master switch pattern specification, played key roles adaptive Significance statement Deciphering genetic underpinnings is fundamental comprehensive understanding evolutionary processes. Long RNAs (lncRNAs) represent emerging category modulators within genome, yet they have overlooked source phenotypic diversity. study, unveil pivotal orchestrating transitions between light development. Remarkably, gene nested region known control multiple cases moths, including iconic natural selection. These findings highlight significant influence lncRNAs regulation, also underscore their potential players process itself.

Language: Английский

Citations

6
Socket Array Irregularities and Wing Membrane Distortions at the Eyespot Foci of Butterfly Wings Suggest Mechanical Signals for Color Pattern Determination DOI Creative Commons

Yugo Nakazato,

Joji M. Otaki

Insects, Journal Year: 2024, Volume and Issue: 15(7), P. 535 - 535

Published: July 16, 2024

Eyespot foci on butterfly wings function as organizers of eyespot color patterns during development. Despite their importance, focal structures have not been examined in detail. Here, we microscopically scales, sockets, and the wing membrane both expanded unexpanded using Blue Pansy

Language: Английский

Citations

3
Antibody-Mediated Protein Knockdown Reveals Distal-less Functions for Eyespots and Parafocal Elements in Butterfly Wing Color Pattern Development DOI Creative Commons

Yugo Nakazato,

Joji M. Otaki

Cells, Journal Year: 2024, Volume and Issue: 13(17), P. 1476 - 1476

Published: Sept. 2, 2024

One of the important genes for eyespot development in butterfly wings is Distal-less. Its function has been evaluated via several methods, including CRISPR/Cas9 genome editing. However, functional inhibition may be performed at right time place using a different method. Here, we used novel protein delivery method pupal wing tissues vivo to inactivate target protein, Distal-less, with polyclonal anti-Distal-less antibody blue pansy Junonia orithya. We first demonstrated that various antibodies were delivered epithelial cells this species. Treatment reduced size, confirming positive role Distal-less development. The treatment eliminated or deformed parafocal element, suggesting element. This result also suggested integrity an and its corresponding element as border symmetry system. Taken together, these findings demonstrate antibody-mediated knockdown useful tool assays proteins, such expressed tissues, functions eyespots elements color pattern

Language: Английский

Citations

3
Wound Healing in Butterfly Pupal Wing Tissues: Real-Time In Vivo Imaging of Long-Range Cell Migration, Cluster Formation, and Calcium Oscillations DOI Creative Commons

Susumu Nagai,

Joji M. Otaki

Insects, Journal Year: 2025, Volume and Issue: 16(2), P. 124 - 124

Published: Jan. 27, 2025

Insects can repair wounds and regenerate body parts in response to physical damage. Wound healing butterfly pupal wing tissues is developmentally interesting because ectopic color patterns develop during healing, suggesting that normal damage-induced may use similar mechanisms. Here we physiologically investigated wound pattern formation using the blue pansy Junonia orithya. In puncture damage, various are formed around damage site. After wounding operation, observed hemocytes migrating over long distances along veins (lacunae) toward site, where epidermal cells cellular clusters. Calcium oscillations were at near transiently affected by ruthenium red, an inhibitor of calcium transporters channels, red caused abnormalities scales adult wings. These results suggest cell migration, cluster formation, play important roles scale development Ectopic local as a consequence evolutionary co-option process for development.

Language: Английский

Citations

0
Spatial mRNA Profiling Using Rapid Amplified Multiplexed-FISH (RAM-FISH) DOI
Tirtha Das Banerjee, J. Dale Raine, Ajay S. Mathuru

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Lepidopteran scale cells derive from sensory organ precursors through a canonical lineage DOI Creative Commons
Ling S. Loh, Kyle A. DeMarr,

Martina Tsimba

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 1, 2024

Abstract The success of butterflies and moths is tightly linked to the origin scales within group. A long-standing hypothesis postulates that are homologous well-described mechanosensory bristles found in fruit fly Drosophila melanogaster , where both derive from an epithelial precursor specified by lateral inhibition then undergoes multiple rounds division. Previous histological examination candidate gene approaches identified parallels genes involved scale bristle development. Here, we provide definitive developmental transcriptomic evidence differentiation lepidopteran derives canonical cell lineage, known as Sensory Organ Precursor (SOP). Live imaging moth butterfly pupae shows SOP cells undergo two asymmetric divisions first abrogate neurogenic lead a differentiated its associated socket cell. Single-nucleus RNA sequencing across time-series early pupal development revealed differential expression patterns mirror lineage development, including Notch-Delta signalling components, adhesion molecules, cycling factors, terminal markers, suggesting shared program. Additionally, recovered novel gene, POU-domain transcription factor pdm3 proper wing scales. Altogether, these data open up avenues for understanding type specification illustrate how single-cell transcriptomics powerful platform evolution types.

Language: Английский

Citations

2