Bulk-level maps of pioneer factor binding dynamics during Drosophila maternal-to-zygotic transition DOI

Sadia Siddika Dima,

Gregory T. Reeves

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Summary Gene regulation by transcription factors (TFs) binding cognate sequences is of paramount importance in development and homeostasis 1–3 . However, quantitative dose/response relationships between bulk TF concentration the DNA binding, an event tied to transcriptional activity, remain elusive. Here, we map these during a crucial step metazoan development: activation zygotic genome. In Drosophila , genome (ZGA) begins with handful genes minor wave ZGA, followed major when thousands are transcribed 4–7 The Zelda (Zld) has ability bind nucleosomal 8,9 subsequently facilitate other TFs 10–16 : two defining features special class known as pioneer 17–19 maternally encoded GAGA factor (GAF) also possesses pioneer-like properties 13,14,20–23 To relationship nuclear performed raster image correlation spectroscopy, method that can measure fluorescent molecules 24–29 We found that, although Zld increases over time, its transcriptionally active regions decreases, consistent function activator for early genes. contrast, GAF nearly linear concentration, which sharply wave, implicating it wave. This study provides key insights into puts forward approach be used regulation.

Language: Английский

Manipulating cell fate through reprogramming: approaches and applications DOI

Masaki Yagi,

Joy E. Horng, Konrad Hochedlinger

et al.

Development, Journal Year: 2024, Volume and Issue: 151(19)

Published: Sept. 30, 2024

ABSTRACT Cellular plasticity progressively declines with development and differentiation, yet these processes can be experimentally reversed by reprogramming somatic cells to induced pluripotent stem (iPSCs) using defined transcription factors. Advances in technology over the past 15 years have enabled researchers study diseases patient-specific iPSCs, gain fundamental insights into how cell identity is maintained, recapitulate early stages of embryogenesis various embryo models, reverse aspects aging cultured animals. Here, we review compare currently available approaches, including factor-based methods small molecule-based derive characteristic embryos. Additionally, discuss our current understanding mechanisms that resist their role maintenance. Finally, recent efforts rejuvenate tissues factors, as well application iPSCs deriving novel models pre-implantation development.

Language: Английский

Citations

2
Bulk-level maps of pioneer factor binding dynamics during Drosophila maternal-to-zygotic transition DOI

Sadia Siddika Dima,

Gregory T. Reeves

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Summary Gene regulation by transcription factors (TFs) binding cognate sequences is of paramount importance in development and homeostasis 1–3 . However, quantitative dose/response relationships between bulk TF concentration the DNA binding, an event tied to transcriptional activity, remain elusive. Here, we map these during a crucial step metazoan development: activation zygotic genome. In Drosophila , genome (ZGA) begins with handful genes minor wave ZGA, followed major when thousands are transcribed 4–7 The Zelda (Zld) has ability bind nucleosomal 8,9 subsequently facilitate other TFs 10–16 : two defining features special class known as pioneer 17–19 maternally encoded GAGA factor (GAF) also possesses pioneer-like properties 13,14,20–23 To relationship nuclear performed raster image correlation spectroscopy, method that can measure fluorescent molecules 24–29 We found that, although Zld increases over time, its transcriptionally active regions decreases, consistent function activator for early genes. contrast, GAF nearly linear concentration, which sharply wave, implicating it wave. This study provides key insights into puts forward approach be used regulation.

Language: Английский

Citations

0