Experimental Cell Research, Journal Year: 2024, Volume and Issue: 444(2), P. 114397 - 114397
Published: Dec. 26, 2024
Language: Английский
The FASEB Journal, Journal Year: 2025, Volume and Issue: 39(4)
Published: Feb. 21, 2025
Abstract Mutations in the septin (SEPT) family lead to male infertility. Septin 14 (SEPT14) is abundantly expressed testis, and its expression significantly reduced individuals with teratozoospermia, suggesting that SEPT14 may play a role spermatogenesis. Here, we demonstrated Sept14 mainly at acroplaxome, manchette, neck, annulus during spermiogenesis. To study of sperm morphogenesis function, knockout ( −/− ) mice were generated. The subfertile presented phenotypes such as irregular acrosomes, DNA damage, disorganized mitochondria, displaced annuli. These abnormalities contributed motility impaired capacitation. Mechanistically, head, interacted colocalized microtubules actin manchette formation metamorphosis phase. In annulus, SEPT9, SEPT7, SEPT2 form filaments maintain localization annulus. GTP‐binding domain (GBD) GBD SEPT2, whereas C‐terminus SEPT7. Thus, our reveals mediating morphogenesis.
Language: Английский
Citations
1
Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 12
Published: Jan. 7, 2025
Infertility affects around 8%-12% of reproductive-aged couples and is a major health concern. Both genetic environmental factors influence male infertility. SEPTIN12 crucial testis-specific gene essential for the final differentiation germ cells strongly linked to infertility due numerous detected mutations. The present study identified novel T96I mutation that causes Immunofluorescence staining transmission electron microscopy (TEM) analysis sperm revealed co-localization SEPT12 SEPT7 in obliquely positioned annulus. In addition, carrying demonstrated large nuclear vacuoles, irregular swelling, decondensation acrosomal cap. overexpression NT2/D1 impaired formation filaments, emphasizing significance filaments morphology function. Our results demonstrate importance offer valuable insights future detection infertile men.
Language: Английский
Citations
0
Reproductive Biology and Endocrinology, Journal Year: 2025, Volume and Issue: 23(1)
Published: Jan. 8, 2025
Heterogeneous nuclear ribonucleoprotein M (HnRNPM) is a key splicing factor involved in various biological processes, including the epithelial‒mesenchymal transition and cancer development. Alternative widely process of spermatogenesis. However, function hnRNPM as during spermatogenesis remains unknown. The expression germ cells at different stages was detected by polymerase chain reaction, western blotting, single-cell database, chromosome spreading assays. Conditional knockout mice were generated to observe development testes male mice. Histological staining, immunofluorescence staining transmission electron microscopy used abnormal sperm from conditional hnRNPM-deficient Coimmunoprecipitation mass spectrometry analyses revealed proteins that interact with hnRNPM. RNA sequencing performed analyse alternative events control In this study, we highly expressed spermatocytes round spermatids, exception XY bodies metaphase. Therefore, cell-specific mouse model investigate role A lack led infertility under natural conditions. Male presented lower numbers sperm, motility, significantly more malformed even tailless sperm. Moreover, found interacted PTBP1 collectively regulate addition, deficiency caused 1617 events, exon skipping Cep152, Cyld, Inpp4b Cd59b. Together, our results suggest regulates mRNAs recruiting required for fertility.
Language: Английский
Citations
0
Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(10)
Published: March 3, 2025
Sperm morphogenesis is a tightly regulated differentiation process, disruption of which leads to sperm malfunction and male infertility. Here, we show that Tex38 knockout (KO) mice are infertile. KO spermatids exhibit excess retention residual cytoplasm around the head, resulting in abnormal morphology with backward head bending. TEX38 interacts colocalizes ZDHHC19, testis-enriched acyltransferase catalyzing protein S-palmitoylation, at plasma membrane spermatids. ZDHHC19 each downregulated mouse testes lacking other protein. stabilizes localizes cultured cells vice versa, consolidating their interdependence. Mice deficient or harboring C142S mutation disables palmitoyltransferase activity display phenotypes resembling those mice. Strikingly, palmitoylates ARRDC5, an arrestin family regulating differentiation. Overall, our findings indicate forms stable complex spermatids, governs downstream S-palmitoylation proteins essential for morphological transformation
Language: Английский
Citations
0
Published: March 3, 2025
Abstract In most animals, spermatids that are produced must further differentiate into fertilization-competent spermatozoa after completing meiosis. Caenorhabditis elegans , this process is known as spermiogenesis or spermatid activation and it results in the transformation of round, non-motile amoeboid, motile spermatozoa. Spermatid C. also associated with fusion Golgi-derived vesicles called m embranous o rganelles (MOs) plasma membrane. This required for producing a surface on sperm by placing MO-carried, resident proteins onto spermatozoon We have identified, cloned, characterized role spe-21 designated dhhc-5, during both hermaphrodite male activation. null mutant worms severely sub-fertile at all studied growth temperatures. fail to activate either vivo vitro treatment chemical activators. found necessary MO pseudopod formation spermatids. The gene encodes predicted four pass transmembrane protein conserved Asp-His-His-Cys (DHHC) tetrapeptide zinc finger motif embedded cysteine rich region (DHHC-CRD type motif). Generally, DHHC-CRD motifs catalyze post-translational addition palmitate their substrates palmitoyltransferases p almitoyl cyltransferases (PATs). mNeonGreen-tagged SPE-21 localizes MOs Together, our findings show an localized palmitoyltransferase proper creation
Language: Английский
Citations
0
Andrology, Journal Year: 2025, Volume and Issue: unknown
Published: April 9, 2025
Abstract Spermatogenesis is a complex differentiation process that facilitated by series of cellular and molecular events. High‐throughput genomics approaches, such as single‐cell RNA sequencing, have begun to enable the systematic characterization these However, loss tissue context because disassociations in isolation protocols limits our ability understand regulation spermatogenesis how defects lead infertility. The recent advancement spatial transcriptomics technologies enables studying signatures various cell types their interactions native context. In this review, we discuss has been leveraged identify spatially variable genes, characterize neighborhood, delineate cell‒cell communications, detect changes under pathological conditions mammalian testis. We believe transcriptomics, along with other emerging resolved omics assays, can be utilized further understanding underlying causes male infertility, facilitate development new treatment approaches.
Language: Английский
Citations
0
Andrology, Journal Year: 2025, Volume and Issue: unknown
Published: April 23, 2025
Abstract Background RIBC1 (RIB43A domain with coiled‐coils 1) and RIBC2 2) are homolog proteins of RIB43a which is localized to microtubules in the cilia flagella unicellular organisms. Cryo‐electron microscopy artificial intelligence studies showed that microtubule inner (MIPs) lumen doublet (DMTs) mouse sperm flagella. However, function mammalian reproduction still unknown. Objective To clarify spermatozoa. Materials methods We generated Ribc1 knockout (KO), Ribc2 KO, double‐knockout ( Ribc1/2 DKO) mice using CRISPR/Cas9 system analyzed their phenotypes. Results revealed loss either or alone did not affect male fertility, but absence both caused a decrease pup numbers. Sperm motility analysis KO spermatozoa had reduced velocity, velocities were comparable WT mice. DKO significantly lower than those from No structural abnormalities axonemal structure at transmission electron microscope (TEM) level no flagellar waveform pattern observed Discussion conclusion Both significant for maintaining spermatozoa, cooperatively motility. This result may indicate minor changes due RIBC protein detected TEM level, depends on think litter size by two proteins.
Language: Английский
Citations
0
Journal of Assisted Reproduction and Genetics, Journal Year: 2025, Volume and Issue: unknown
Published: April 24, 2025
Language: Английский
Citations
0
Cellular and Molecular Life Sciences, Journal Year: 2025, Volume and Issue: 82(1)
Published: May 2, 2025
Spermatogenesis is the basis of sexual reproduction and essential for propagation insect populations. Understanding process spermatogenesis identifying key genes involved in sperm function can aid developing pest genetic control methods. The testis-specific gene β2-tubulin (B2t) crucial insects possessing monomorphic spermatids. However, role B2t lepidopteran dimorphic remains unclear. In this study, we explored effect development eupyrene apyrene Spodoptera frugiperda, a major global pest. Knockout resulted male sterility. mutations lead to abnormal inability transfer from testis double ejaculatory ducts. disruption did not affect migration. Interestingly, found that first mating with B2t-null males inhibited fertilization second wild-type male. Cage studies mathematical modeling analyses suggested releasing excessive suppressed female fertility. Our study provides insights into pests an efficient molecular target techniques.
Language: Английский
Citations
0
Neuropharmacology, Journal Year: 2024, Volume and Issue: 265, P. 110271 - 110271
Published: Dec. 16, 2024
Language: Английский
Citations
0