Evaluation of carp sperm respiration: fluorometry with optochemical oxygen sensor versus polarography DOI Creative Commons
И. Б. Мусатова, Borys Dzyuba, Sergii Boryshpolets

et al.

Fish Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 51(1), P. 1 - 14

Published: Dec. 4, 2024

Abstract The primary function of spermatozoa is to fertilize the oocyte, which depends on their motility and directly associated with metabolic state. oxygen consumption rate (OCR) reflects respiratory capacity sperm mitochondria under various physiological conditions an essential marker quality. We determined OCR common carp ( Cyprinus carpio ) using two respirometry methods: conventionally used polarographic method a Clark-type electrode fluorometric assay Oxo Dish optochemical sensor. latter was for first time evaluate in states (under different treatments) at dilution rates. These methods were compared Bland–Altman analysis, applicability sensor evaluating discussed. Sperm progressive velocity parameters also assessed effect respiration rates preincubation period status spermatozoa. comparison these clearly shows that while allows immediate measurement levels after adding sample, has priority amount data obtained due simultaneous measurements several samples (e.g., males, fish species, repetitions same sample or experimental conditions), even later measuring chamber. However, are complementary, proposed methodology can be applied other species.

Language: Английский

Per capita sperm metabolism is density dependent DOI Creative Commons
Ashley E. Potter, Craig R. White, Dustin J. Marshall

et al.

Journal of Experimental Biology, Journal Year: 2024, Volume and Issue: 227(6)

Published: Feb. 21, 2024

ABSTRACT From bacteria to metazoans, higher density populations have lower per capita metabolic rates than populations. The negative covariance between population and rate is thought represent a form of adaptive plasticity. A relationship metabolism was actually first noted 100 years ago, focused on spermatozoa; even then, it postulated that plasticity drove this pattern. Since contemporary studies sperm specifically assume concentration has no effect show We did systematic review estimate the aerobic concentration, for 198 estimates spanning 49 species, from protostomes humans 88 studies. found strong evidence are dependent: both within among metabolisms in dense ejaculates, but increase their when diluted. On average, 10-fold decrease increased by 35%. Metabolic appears be an response, whereby maximize chances encountering eggs.

Language: Английский

Citations

2
Evaluation of carp sperm respiration: fluorometry with optochemical oxygen sensor versus polarography DOI Creative Commons
И. Б. Мусатова, Borys Dzyuba, Sergii Boryshpolets

et al.

Fish Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 51(1), P. 1 - 14

Published: Dec. 4, 2024

Abstract The primary function of spermatozoa is to fertilize the oocyte, which depends on their motility and directly associated with metabolic state. oxygen consumption rate (OCR) reflects respiratory capacity sperm mitochondria under various physiological conditions an essential marker quality. We determined OCR common carp ( Cyprinus carpio ) using two respirometry methods: conventionally used polarographic method a Clark-type electrode fluorometric assay Oxo Dish optochemical sensor. latter was for first time evaluate in states (under different treatments) at dilution rates. These methods were compared Bland–Altman analysis, applicability sensor evaluating discussed. Sperm progressive velocity parameters also assessed effect respiration rates preincubation period status spermatozoa. comparison these clearly shows that while allows immediate measurement levels after adding sample, has priority amount data obtained due simultaneous measurements several samples (e.g., males, fish species, repetitions same sample or experimental conditions), even later measuring chamber. However, are complementary, proposed methodology can be applied other species.

Language: Английский

Citations

0