Determination of Stable Reference Genes for Gene Expression Analysis in Black Rockfish (Sebastes schlegeli) Under Hypoxia Stress DOI Open Access

Xiatian Chen,

Yujie Yu, Tao Gao

et al.

Genes, Journal Year: 2024, Volume and Issue: 16(1), P. 9 - 9

Published: Dec. 25, 2024

Hypoxia triggers stress, leading to significant alterations in gene expression patterns, which turn affect fish's growth and development. Real-time quantitative PCR (RT-qPCR) is a pivotal technique for assessing changes expression. However, its accuracy highly contingent upon the stable of reference genes. Ribosomal RNA (18s), β-actin (actb), elongation factor 1-α (ef1a), α tubulin (tuba), ribosomal protein L17 (rpl17) are widely used genes, but their stability tissues black rockfish under hypoxic conditions remains unclear. The genes was detected by RT-qPCR assessed Delta Ct, geNorm, NormFinder, BestKeeper algorithms. Results showed that tuba exhibited liver, heart, gill normoxic conditions, liver head kidney conditions. Ef1a identified as most stably expressed tissue hypoxia. For heart studies, rpl17 were recommended 18s high spleen Actb tissue-specific stability, it necessary select appropriate based on specific type studies These findings help enhancing analysis mechanism hypoxia rockfish.

Language: Английский

Contrasting thermal and hypoxic responses of species from blackwater and whitewater rivers DOI
Susana Braz‐Mota, Rafael Mendonça Duarte, Adalberto Luís Val

et al.

Journal of Fish Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Abstract In the Amazon basin, climate change is increasing water temperatures and CO₂ concentrations, which intensify environmental stressors for fish by natural thermal regimes, reducing dissolved oxygen pH levels. Extreme droughts, such as those recorded in 2023 new record set 2024, have exacerbated these conditions led to widespread mortality. Amazonian rivers, are characterized contrasting whitewater blackwater systems, provide unique habitats with distinct pHs that shape biodiversity species adaptations. With regional projected increase 2.2–7.0°C end of century, understanding expected vulnerability has become crucial. This study aims investigate influence type (blackwater vs. whitewater) on both hypoxia tolerances responses different following extreme drought analyse individual taxonomic The results revealed significant variability among species, displaying a lower safety margin blackwaters. observed between fishes highlight complexity their adaptive strategies response fundamental predicting future environment under current scenario.

Language: Английский

Citations

1
Worsening Drought and the Bleak Future for Biodiversity and Ecosystem Services in the Amazon Basin DOI Open Access
Janeide Padilha,

Cláudio E. Azevedo-Silva,

Cláudio Ernesto Taveira Parente

et al.

Aquatic Conservation Marine and Freshwater Ecosystems, Journal Year: 2025, Volume and Issue: 35(3)

Published: March 1, 2025

Language: Английский

Citations

0
How do extreme fluctuations in water level affect fish condition in Amazonian Floodplain Lakes? DOI Open Access
Priit Zingel, Arvo Tuvikene, Tiina Zingel

et al.

Journal of Fish Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Abstract The annual flood pulse is a defining feature of Amazonian floodplain lakes, creating highly variable environment that influences resource availability, such as food and habitat. These cyclical changes necessitate high degree adaptability among fish species, many which have evolved specialized strategies to cope with the fluctuating conditions. In 2023, Amazon basin experienced record‐breaking drought event, leading mass mortality other wildlife. This study examines effect this extreme event on condition in white‐water (Rio Solimões basin) black‐water Negro lakes. contrasting environments provide unique opportunity how different water qualities water‐level fluctuations impact condition. Research was conducted during normal low‐water period November 2019 drastically decreased levels 2023. main objective understand affect health nutritional status. A total 585 fishes were analyzed, 294 from 291 black‐water, representing feeding types comprehensive picture Water‐level had statistically significant both areas. Comparing levels, consistently higher period. linear mixed‐effects model revealed intensity season length‐adjusted mass, suggesting decrease level associated an overall mass. When comparing mean (Glass's Δ ) between we found bigger system than system. difference may be attributed lower nutrient content humic acids refractory dissolved organic matter can further limit primary productivity availability for fishes.

Language: Английский

Citations

0
Artisanal Fishing in the Lower Amazon Under Scrutiny: an Analysis Through the Climate Lens DOI
Raoni Fernandes Azerêdo, Cláudio Fabian Szlafsztein

Revista de Gestão Social e Ambiental, Journal Year: 2024, Volume and Issue: 18(11), P. e09996 - e09996

Published: Nov. 25, 2024

Objective: The objective of this study is to analyze the impacts extreme hydrological drought 2023 on artisanal fishing in Lower Amazon Integration Region and its threats development Bioeconomy Plan for state Pará, understand how production chain vulnerable risks arising from climate variability change. Theoretical Framework: In order vulnerability fisheries change, as well propose adaptive strategies, Climate Lens tool was applied (OECD, 2009; Carvalho et al., 2021). Method: methodology adopted included gathering data information reports non-governmental agencies technical opinions municipal civil defense departments. Semi-structured interviews were also conducted with directors fishermen's colonies officials Results Discussion: results obtained revealed that, municipalities, without access income, drinking water, food facing social isolation, has become impractical due low water levels rivers lakes, resulting an incalculable fish die-off. [synthesize main research]. discussion section, these are contextualized light theoretical framework, highlighting implications relationships identified. Possible discrepancies limitations considered section. Research Implications: Based results, measures recommended that can be implemented response existing and/or potential impacts. Originality/Value: This contributes literature by solutions scenario experienced implementation Pará.

Language: Английский

Citations

0
Preface: Secrets of shallow lakes—insights from research DOI Creative Commons
Priit Zingel,

María Boveri,

Helen Agasild

et al.

Hydrobiologia, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 22, 2024

A sad example is the Amazon in Brazilas these lines are written, region experiencing unprecedented droughts for second consecutive year, resulting many floodplain lakes drying up or disappearing (Braz-Mota & Val, 2024).It cannot be ruled out that reaching a predicted tipping point, where forest degradation attains point of no return, and area degrades into grassy savanna.This plausible hypothesis, other biomes also threatened by equivalent dangerous thresholds.And all them, before catastrophe this nature hits, aquatic ecosystems ones ring alarm.Therefore, it crucial to monitor health water bodies-nature will always manage, but humans who need environment around us remain healthy.We must not forget protecting about ourselves.

Language: Английский

Citations

0
Determination of Stable Reference Genes for Gene Expression Analysis in Black Rockfish (Sebastes schlegeli) Under Hypoxia Stress DOI Open Access

Xiatian Chen,

Yujie Yu, Tao Gao

et al.

Genes, Journal Year: 2024, Volume and Issue: 16(1), P. 9 - 9

Published: Dec. 25, 2024

Hypoxia triggers stress, leading to significant alterations in gene expression patterns, which turn affect fish's growth and development. Real-time quantitative PCR (RT-qPCR) is a pivotal technique for assessing changes expression. However, its accuracy highly contingent upon the stable of reference genes. Ribosomal RNA (18s), β-actin (actb), elongation factor 1-α (ef1a), α tubulin (tuba), ribosomal protein L17 (rpl17) are widely used genes, but their stability tissues black rockfish under hypoxic conditions remains unclear. The genes was detected by RT-qPCR assessed Delta Ct, geNorm, NormFinder, BestKeeper algorithms. Results showed that tuba exhibited liver, heart, gill normoxic conditions, liver head kidney conditions. Ef1a identified as most stably expressed tissue hypoxia. For heart studies, rpl17 were recommended 18s high spleen Actb tissue-specific stability, it necessary select appropriate based on specific type studies These findings help enhancing analysis mechanism hypoxia rockfish.

Language: Английский

Citations

0