Nature Communications,
Journal Year:
2018,
Volume and Issue:
9(1)
Published: Oct. 8, 2018
The
turnover
of
brain
proteins
is
critical
for
organism
survival,
and
its
perturbations
are
linked
to
pathology.
Nevertheless,
protein
lifetimes
have
been
difficult
obtain
in
vivo.
They
readily
measured
vitro
by
feeding
cells
with
isotopically
labeled
amino
acids,
followed
mass
spectrometry
analyses.
In
vivo
generated
from
at
least
two
sources:
acids
the
diet,
non-labeled
degradation
pre-existing
proteins.
This
renders
measurements
difficult.
Here
we
solved
this
problem
rigorously
a
workflow
that
combines
mouse
isotopic
labeling,
spectrometry,
mathematical
modeling.
We
also
established
several
independent
approaches
test
validate
results.
enabled
us
measure
accurate
~3500
high
precision
our
data
provided
large
set
biologically
significant
observations,
including
pathway-,
organelle-,
organ-,
or
cell-specific
effects,
along
comprehensive
catalog
extremely
long-lived
(ELLPs).
Nano Letters,
Journal Year:
2010,
Volume and Issue:
10(4), P. 1297 - 1301
Published: March 1, 2010
A
memristor
is
a
two-terminal
electronic
device
whose
conductance
can
be
precisely
modulated
by
charge
or
flux
through
it.
Here
we
experimentally
demonstrate
nanoscale
silicon-based
and
show
that
hybrid
system
composed
of
complementary
metal−oxide
semiconductor
neurons
synapses
support
important
synaptic
functions
such
as
spike
timing
dependent
plasticity.
Using
memristors
in
neuromorphic
circuits
potentially
offer
both
high
connectivity
density
required
for
efficient
computing.
Science,
Journal Year:
2017,
Volume and Issue:
357(6357)
Published: Aug. 11, 2017
Hundreds
of
circular
RNAs
(circRNAs)
are
highly
abundant
in
the
mammalian
brain,
often
with
conserved
expression.
Here
we
show
that
circRNA
Cdr1as
is
massively
bound
by
microRNAs
(miRNAs)
miR-7
and
miR-671
human
mouse
brains.
When
locus
was
removed
from
genome,
knockout
animals
displayed
impaired
sensorimotor
gating-a
deficit
ability
to
filter
out
unnecessary
information-which
associated
neuropsychiatric
disorders.
Electrophysiological
recordings
revealed
dysfunctional
synaptic
transmission.
Expression
specifically
posttranscriptionally
misregulated
all
brain
regions
analyzed.
immediate
early
genes
such
as
Fos,
a
direct
target,
enhanced
Cdr1as-deficient
brains,
providing
possible
molecular
link
behavioral
phenotype.
Our
data
indicate
an
vivo
loss-of-function
phenotype
suggest
interactions
between
miRNAs
important
for
normal
function.
Advances In Physics,
Journal Year:
2011,
Volume and Issue:
60(2), P. 145 - 227
Published: Feb. 10, 2011
Memory
effects
are
ubiquitous
in
nature
and
particularly
relevant
at
the
nanoscale
where
dynamical
properties
of
electrons
ions
strongly
depend
on
history
system,
least
within
certain
time
scales.
We
review
here
memory
various
materials
systems
which
appear
most
strikingly
their
non-trivial
time-dependent
resistive,
capacitative
inductive
characteristics.
describe
these
characteristics
framework
memristors,
memcapacitors
meminductors,
namely
circuit
elements
whose
state
system.
examine
basic
issues
related
to
such
critically
report
both
theoretical
experimental
progress
understanding
functionalities.
also
discuss
possible
applications
areas
science
technology
ranging
from
digital
analog
electronics,
biologically-inspired
circuits,
learning.
finally
future
research
opportunities
field.
Journal of Neuroscience,
Journal Year:
2013,
Volume and Issue:
33(32), P. 13204 - 13224
Published: Aug. 7, 2013
The
spatiotemporal
organization
of
neurotransmitter
receptors
in
postsynaptic
membranes
is
a
fundamental
determinant
synaptic
transmission
and
information
processing
by
the
brain.
Using
four
independent
super-resolution
light
imaging
methods
EM
genetically
tagged
endogenous
receptors,
we
show
that,
rat
hippocampal
neurons,
AMPARs
are
often
highly
concentrated
inside
synapses
into
few
clusters
∼70
nm
that
contain
∼20
receptors.
stabilized
reversibly
these
nanodomains
diffuse
freely
outside
them.
Nanodomains
dynamic
their
shape
position
within
can
form
or
disappear
minutes,
although
they
mostly
stable
for
up
to
1
h.
AMPAR
often,
but
not
systematically,
colocalized
with
scaffold
protein
PSD95,
which
generally
larger
size
than
nanoclusters.
PSD95
expression
level
regulates
nanodomain
compactness
parallel
miniature
EPSC
amplitude.
Monte
Carlo
simulations
further
indicate
impact
concentration
on
efficacy
transmission.
observation
nanodomains,
instead
diffusively
distributed
PSD
as
thought,
has
important
consequences
our
understanding
excitatory
neurotransmission.
Furthermore,
results
glutamatergic
controlled
nanometer-scale
regulation
nanodomains.
Proceedings of the National Academy of Sciences,
Journal Year:
2010,
Volume and Issue:
107(5), P. 1870 - 1875
Published: Jan. 11, 2010
A
generalized
platform
for
introducing
a
diverse
range
of
biomolecules
into
living
cells
in
high-throughput
could
transform
how
complex
cellular
processes
are
probed
and
analyzed.
Here,
we
demonstrate
spatially
localized,
efficient,
universal
delivery
immortalized
primary
mammalian
using
surface-modified
vertical
silicon
nanowires.
The
method
relies
on
the
ability
nanowires
to
penetrate
cell’s
membrane
subsequently
release
surface-bound
molecules
directly
cytosol,
thus
allowing
highly
efficient
without
chemical
modification
or
viral
packaging.
This
modality
enables
one
assess
phenotypic
consequences
broad
biological
effectors
(DNAs,
RNAs,
peptides,
proteins,
small
molecules)
almost
any
cell
type.
We
show
that
this
can
be
used
guide
neuronal
progenitor
growth
with
molecules,
knock
down
transcript
levels
by
delivering
siRNAs,
inhibit
apoptosis
introduce
targeted
proteins
specific
organelles.
further
codelivery
siRNAs
single
substrate
microarray
format,
highlighting
technology’s
potential
as
robust,
monolithic
high-throughput,
miniaturized
bioassays.
