The m6A reader ECT8 is an abiotic stress sensor that accelerates mRNA decay in Arabidopsis

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(8), P. 2908 - 2926

Published: June 5, 2024

Abstract N 6-methyladenosine (m6A) is the most abundant mRNA modification and plays diverse roles in eukaryotes, including plants. It regulates various processes, plant growth, development, responses to external or internal stress responses. However, mechanisms underlying how m6A related environmental stresses both mammals plants remain elusive. Here, we identified EVOLUTIONARILY CONSERVED C-TERMINAL REGION 8 (ECT8) as an reader protein showed that its m6A-binding capability required for salt Arabidopsis (Arabidopsis thaliana). ECT8 accelerates degradation of target transcripts through direct interaction with decapping DECAPPING 5 within processing bodies. We observed a significant increase expression level under stresses. Using representative stressor, found transcript levels rise response stress. The increased abundance results enhanced binding m6A-modified mRNAs, thereby accelerating their degradation, especially those negative regulators Our demonstrated acts abiotic sensor, facilitating decay, which vital maintaining transcriptome homeostasis enhancing tolerance findings not only advance understanding epitranscriptomic gene regulation but also offer potential applications breeding more resilient crops face rapidly changing conditions.

Language: Английский

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Crosstalk between RNA m6A modification and epigenetic factors in plant gene regulation

Plant Communications, Journal Year: 2024, Volume and Issue: 5(10), P. 101037 - 101037

Published: July 6, 2024

N6-methyladenosine (m6A) is the most abundant modification observed in eukaryotic mRNAs. Advances transcriptome-wide m6A mapping and sequencing technologies have enabled identification of several conserved motifs plants, including RRACH (R = A/G H A/C/U) UGUAW (W U or A) motifs. However, mechanisms underlying deposition marks at specific positions individual transcripts remain to be clarified. Evidence from plant animal studies suggests that writer eraser components are recruited genomic loci through interactions with particular transcription factors, 5-methylcytosine DNA methylation marks, histone marks. In addition, recent cells shown microRNAs play a role depositing sites base-pairing mechanism. also affects biogenesis function chromatin-associated regulatory RNAs long noncoding RNAs. Although we less an understanding link between epigenetic factors plants than animals, progress identifying proteins interact has provided insights into crosstalk which plays crucial transcript-specific regulation plants.

Language: Английский

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Epigenetics in the modern era of crop improvements

Science China Life Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Abstract Epigenetic mechanisms are integral to plant growth, development, and adaptation environmental stimuli. Over the past two decades, our comprehension of these complex regulatory processes has expanded remarkably, producing a substantial body knowledge on both locus-specific genome-wide patterns. Studies initially grounded in model Arabidopsis have been broadened encompass diverse array crop species, revealing multifaceted roles epigenetics physiological agronomic traits. With recent technological advancements, epigenetic regulations at single-cell level large-scale population emerging as new focuses. This review offers an in-depth synthesis regulations, detailing catalytic machinery functions. It delves into intricate interplay among various elements their collective influence modulation Furthermore, it examines breakthroughs technologies for modifications integration strategies improvement. The underscores transformative potential bolstering performance, advocating development efficient tools fully exploit agricultural benefits insights.

Language: Английский

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Tomato (Solanum lycopersicum L.) YTH Domain-Containing RNA-Binding Protein (YTP) Family Members Participate in Low-Temperature Treatment and Waterlogging Stress Responses

Horticulturae, Journal Year: 2024, Volume and Issue: 10(5), P. 522 - 522

Published: May 17, 2024

YT521-B homology (YTH) domain-containing RNA-binding proteins (YTPs) are important N6-methyladenosine (m6A) readers that have crucial roles in determining the destiny of m6A-modified RNAs, which most widespread RNA modifications eukaryotes. Tomatoes (Solanum lycopersicum L.) hold significant importance both dietary consumption patterns and scientific inquiries. While YTP gene family has been characterized tomatoes, their specific reactions to low temperature waterlogging stresses remain be elucidated. In our study, nine tomato SlYTPs could divided into five subclasses, YTHDFa-c YTHDCa-b. After cloning measuring expression levels under stress conditions, it was revealed SlYTP8 exhibited increased sensitivity low-temperature treatment, while SlYTP9 were notably upregulated leaf tissues subjected conditions. As members YTHDFc subfamily, localized cytoplasm. Nevertheless, overexpression (OE) plants manifested by a higher accumulation malondialdehyde (MDA) hydrogen peroxide (H2O2) weaker reactive oxygen species scavenging ability compared wild-type (WT) tomatoes. However, comparison WT plants, leaves OE tomatoes showed chlorophyll content stronger after 3 days thereby increasing resistance stress. Moreover, order investigate possible molecular mechanisms underlying responses stresses, transcription factors interacting protein networks associated with SlYTP8/9 promoters also predicted, respectively. These results fill gap understanding YTPs response providing theoretical experimental basis for subsequent studies on mechanisms.

Language: Английский

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Dynamic Changes in Tomato (Solanum lycopersicum) RNA m6A Modification During Seed Germination and Under Microgravity Conditions

Horticulturae, Journal Year: 2025, Volume and Issue: 11(3), P. 282 - 282

Published: March 5, 2025

Research exploring involvement of RNA N6-methyladenosine (m6A) in tomato (Solanum lycopersicum) seed germination remains limited. There is also a lack direct evidence supporting the interaction among germination, microgravity, and m6A modification. In this study, Micro-Tom tomatoes are used as experimental material to conduct genetic transformation, assay, modification levels identification experiments. During processes, level significantly increases under mutual influence various methyltransferase subunits multiple eraser proteins. As reader gene, SlYTP9 expression affects seeds, with promotion inhibition OE (overexpression) RNAi (RNA interference) transgenic plants, respectively. Microgravity promotes early period (0–3 days past germination; 0–3 DPG), but promoting effect gradually disappears seedling grows (8–15 DPG). Further exploration revealed that correlated modification, manifested enhanced most writer genes; increased overall altered trends some genes, particularly SlALKBH2; levels. The results obtained study can provide theoretical basis support for elucidating role well interactions between

Language: Английский

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A comprehensive review of m6A modification in plant development and potential quality improvement

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142597 - 142597

Published: March 1, 2025

Language: Английский

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Functions of Tomato (Solanum lycopersicum L.) Signal Transducer and Activator of Transcription (STAT) in Seed Germination and Low-Temperature Stress Response

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3338 - 3338

Published: April 3, 2025

Tomato (Solanum lycopersicum L.) is one of the major vegetable crops worldwide. Research on Janus kinase-signal transducer and activator transcription (JAK-STAT) signaling pathway in tomatoes other plant systems extremely limited. In this study, roles STAT, a crucial element JAK-STAT tomato seed germination low-temperature stress responses are examined, employing gene family analysis genetic transformation. The results indicate that S. genome contains only member STAT family, SlSTAT. Subcellular localization experiments reveal SlSTAT found both cytoplasm nucleus, suggesting its potential involvement biological functions within these cellular compartments. Among 26 different tissue/organs tested, exhibited higher expression levels hypocotyl (8 days past germination; 8 DPG), low significantly reduced rate impacted biomass at DPG. addition, was downregulated during treatment. Compared with wild-type (WT) tomatoes, SlSTAT-overexpressing plants showed more resistance to conditions, whereas downexpressing increased sensitivity. expressions marker genes (SlCBF1-3) N6-methyladenosine (m6A)-modification-related (m6A writer, reader, eraser genes) were detected explore possible molecular mechanisms by which causes changes resistance. SlCBF1-3 transgenic do not merely follow straightforward linear relationship expression, complex mechanism non-direct interaction between promoters SlCBFs. On hand, also RNA m6A-modification-related genes, especially SlFIP37 (writer gene), SlYTP8/9 (reader genes), SlALKBH8 (eraser ultimately leading m6A modification. These research findings lay groundwork for exploring development responses, expanding scope studies systems.

Language: Английский

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